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Trials and Tribulations of a First Year iGEM Team
- 1. Trials and Tribulations of a First Year iGEM Team Morgan Taschuk Newcastle University
- 4. Potholes
- 5. The Jamboree
- 6. Happy Ending
- 7. Questions
- 8. The Team Advisors Instructors Students
- 10. And me
- 11. Wanted to shoot big!
- 12. Circle of (Synthetic) Life Feedback Implementation Computational Modelling Bioinformatics Tools Sequence Synthesize Clone Analyze
- 14. Not “sexy”
- 21. Need to discriminate between fingerprints
- 23. Need to discriminate between fingerprints
- 24. Limited outputs
- 26. Where do we start??
- 28. Wet lab booked from 4 August
- 30. Designed a BioBrick by hand
- 32. Bad timing May Jun Jul Aug Sept Oct Nov Apr
- 33. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team
- 34. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team
- 35. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team
- 36. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team Tasks
- 37. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team Tasks
- 38. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team Tasks
- 39. Bad timing May Jun Jul Aug Sept Oct Nov Apr Team Tasks
- 41. Number of remaining students: 1
- 43. T-Shirts
- 44. Presentation
- 45. DNA submission
- 46. Only just about everything we're judged on!
- 48. Ripped several theses apart
- 50. T-Shirts David Appleyard and iGEM
- 52. Not all parts need to have DNA, or even work!
- 55. Practiced with the first version on 4 November
- 57. Seven hour layover in Amsterdam
- 58. Revised the talk (again)
- 59. Stata Center
- 61. Lots going on David Appleyard and iGEM
- 62. People Watching
- 63. David Appleyard and iGEM
- 66. ?
- 73. Poster Session David Appleyard and iGEM Don’t let this happen to you!
- 74. Afters David Appleyard and iGEM
- 76. Caltech
- 77. Slovenia
- 78. Freiburg
- 79. Harvard
- 80. UC Berkeley
- 81. Awards David Appleyard and iGEM
- 82. iGEM from Overhead David Appleyard and iGEM Us!
- 83. Melee onstage David Appleyard and iGEM
- 84. Melee David Appleyard and iGEM
- 85. Awards
- 87. Communication!
- 88. Have a contingency plan
- 89. You have less time than you think you have
- 92. Bio brick away!
- 93. Thank you and good luck!
Notes de l'éditeur
- First year iGEM – wanted to shoot big! Wanted something awesome, on
- Methicillin-resistant Staphylococcus aureus MRSA difficult to distinguish from other S. aureus Mostly they just grow up the culture and see if they die in response to methicillin and other antibiotics Not sexy, as one of our advisors called it! It's not big and awesome enough for iGEM. Can be accomplished using traditional biology No computational aspect. Most of instructors were from computing science, with interest in synthetic biology. Therefore, wanted to incorporate in silico with biology
- Quorum sensing Fingerprints: unique to species or even the strain Cell-to-cell communication, determine friend or foe Multiple detection Pull quorum self-sensing systems out of multiple bacteria and place it into a single B. subtilis genome Fingerprints can be very similar. Related strains may release the same signal peptides, but at different relative levels Limited outputs Practical consideration: only 3-4 fluorescent proteins can be used with any chance of detecting by eye
- Quorum sensing Fingerprints: unique to species or even the strain Cell-to-cell communication, determine friend or foe Multiple detection Pull quorum self-sensing systems out of multiple bacteria and place it into a single B. subtilis genome Fingerprints can be very similar. Related strains may release the same signal peptides, but at different relative levels Limited outputs Practical consideration: only 3-4 fluorescent proteins can be used with any chance of detecting by eye
- Quorum sensing Fingerprints: unique to species or even the strain Cell-to-cell communication, determine friend or foe Multiple detection Pull quorum self-sensing systems out of multiple bacteria and place it into a single B. subtilis genome Fingerprints can be very similar. Related strains may release the same signal peptides, but at different relative levels Limited outputs Practical consideration: only 3-4 fluorescent proteins can be used with any chance of detecting by eye
- Quorum sensing Fingerprints: unique to species or even the strain Cell-to-cell communication, determine friend or foe Multiple detection Pull quorum self-sensing systems out of multiple bacteria and place it into a single B. subtilis genome Fingerprints can be very similar. Related strains may release the same signal peptides, but at different relative levels Limited outputs Practical consideration: only 3-4 fluorescent proteins can be used with any chance of detecting by eye
- Quorum sensing Fingerprints: unique to species or even the strain Cell-to-cell communication, determine friend or foe Multiple detection Pull quorum self-sensing systems out of multiple bacteria and place it into a single B. subtilis genome Fingerprints can be very similar. Related strains may release the same signal peptides, but at different relative levels Limited outputs Practical consideration: only 3-4 fluorescent proteins can be used with any chance of detecting by eye
- Learned: Be very clear about commitments Communication! Have a contigency plan
- No computational tools were ready: Masters projects ran until beginning of August. Wet lab booked at the beginning of August. While this timetable initially seemed reasonable, there is an additional step between BioBrick design and testing it in the bacteria: DNA production. Since we were using B. subtilis, and our brick wasn't in the repository, opted to have it synthesized Designed a Brick by hand
- Mostly sandwiches, crisps, fruit Bagels for breakfast – I thought it was great, but some people were less pleased
- Although we considered the lack of real questions at the end a vicopry, we suspected it had to do with two things: 1) Our project was mostly a software project, but we tried hard to make it fit into another track 2) We didn't make any grandiose claims about curing cancer in order to enhance our presentation – we just told it like it was.