extraction of polyphenol from pomegranate

2. Extraction Of Polyphenol
From Pomegranate

3. Introduction
Polyphenols:
Phenolic compounds are natural antioxidants present in plants
which are important to human health.
Benefits of Polyphenols:
 Prevent Cardiovascular Disease
 Reduce Inflammation
 Lower The Incidence of Cancers
 Diabetes Mellitus
 Reduce Rates of Mutagenesis
 Reduce Obesity
 Reduce Hypertension

4. MATERIALS AND METHODS
 Pomegranate was collected and
grinded.
Grinded powder was packed in air
tight polythene.
It was stored in refrigerator for
further analysis.
Extraction Of Polyphenol From
Pomegranate

5. Sample Preparation
 pomegranate peel powder was taken in conical flask
 sample to solvent ratio was 1:10.
 ethanol was used as a solvent.
 Conical flask was placed in TECATOR Shaking Water Bath for maceration.
 After maceration it was filtered through whatman filter paper no. 41.
 Filtrate was taken at BUCHI ROTAVAPOR to evaporate the solvent.
 Then it was taken in dry Amber glass bottle and stored for determination
of Total Polyphenols.

6. The proximate analysis of pomegranate peels powder results were shown in below the table. The
moisture (%) content was 04+ 0.22, the ash content (%) was 05 +0.14 fat (%) content was 9.4 ±
0.1, similarly the pH, was found 3.75 ± 0.2, . Crude fiber was calculated (21 ± 0.6).
S# Proximate analysis Results
1. Moisture 04 ± 0.22(%)
2. Ash 05 ± 0.14(%)
3. Fat 9.4 ± 0.1(%)
4. pH 3.75 ± 0.2
5. Crude Fiber 21 ± 0.6(%)
6. Protein 8.719± 0.10(%)
7. Nitrogen 1.395± 0.30(%)
RESULTS AND DISCUSSION

7. Total polyphenols Content of Pomegranate Peels
In replication I we obtain 54 and in rep II and III we have gotten 56, 57 respectively mean of
these three rep calculated was 55.67 with standard deviation of + 2.12
Sample Total polyphenol Content(mg/g gallic acid equivalent)
Rep I 54
Rep II 56
Rep III 57
Mean 55.67+ 2.12

8. Total polyphenol in pomegranate

9. Instruments Used for Extraction Phenolic
Compound
Rotavapor

10. Instruments Used for Extraction Phenolic
Compound
Glassware

11. Instruments Used for Extraction Phenolic
Compound
Weighing Balance:

12. Instruments Used for Extraction Phenolic
Compound
Water Shaking Bath

13. Instruments Used for Extraction Phenolic
Compound
Hot air Oven

14. UV- VIS Spectroscopy
To measure the amount of Polyphenol compounds in the sample.

15. Muffle Furnace
Muffle furnace is used for ashing of product or sample.