Forensics

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An STR genotype is the allele, in the case of a homozygote, or alleles, in the case of a heterozygote, present in a sample for a particular locus and is normally reported as the number of repeats present in the allele. A full sample genotype or STR profi le is produced by the combination of all of the locus genotypes into a single series of numbers. This profi le is what is entered into a case report or a DNA database for comparison purposes to other samples.

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Sequencing DNA means determining the order of the four chemical building blocks - called "bases" - that make up the DNA molecule. The sequence tells scientists the kind of genetic information that is carried in a particular DNA segment. For example, scientists can use sequence information to determine which stretches of DNA contain genes and which stretches carry regulatory instructions, turning genes on or off. In addition, and importantly, sequence data can highlight changes in a gene that may cause disease.

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Forensic science is defined as the application of scientific knowledge and experimentation to legal contentions, be they civil or criminal matters. DNA profiling (also called DNA typing or DNA fingerprinting) is a forensic techniques used to identify individuals by characteristics of their DNA in crime cases. DNA profiling can be use to resolve paternal and ancestral issues. This process was built mainly on the knowledge of two scientific breakthroughs. First is the Polymerase Chain Reaction (PCR) which was conceived by Kary Mullis in 1983 at Cetus Corporation, USA. Second is the Restriction Fragment Length Polymorphism (RFLP) analysis of repeated DNA sequences which was discovered by Professor Sir Alec Jeffreys in 1985 at the University of Leicester, UK. The strengths and limitations of the current and emerging forensic DNA profiling are the focus of this seminar. It is my expectation that the newly proposed synthetic human genome project will aid the strength of this process in the future.

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The so-called “next-generation” sequencing (NGS) technologies allows us, in a short time and in parallel, to sequence massive amounts of DNA, overcoming the limitations of the original Sanger sequencing methods used to sequence the first human genome. NGS technologies have had an enormous impact on biomedical research within a short time frame. This talk will give an overview of these applications with specific examples from Mendelian genomics and cancer research. #h2ony

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Instructions W4 Nightingale Case A & B – 35 points - Individual Assignment As indicated in the syllabus, it is important to demonstrate knowledge of MS Project. Week 4 includes using the software and interpreting the results as follows: 1. Read the Nightingale Project - LG textbook pg 333-335 2. Review MS Project Video Tutorials (Lessons/Course Materials/Support Videos) and complete the Case for both Part A and Case Part B. 3. Submit two separate MS Project .mpp files (one for part A and one for part B). Remember to submit the appropriate “view” reflecting all applicable columns and content information. 4. Submit MS Word file to specifically answer all questions for both parts (part A questions 1-3 & part B questions 1-4). 5. Ensure you document the version of MS Project you are using in the submission comments field. Hints: You should read ALL instructions in the case and case technical details before you start the Project file. You may want to set up the Project file ex: start date, holidays, work days, etc. before entering in any tasks. Ensure the project name is on the first line of the Project file and all other tasks as detailed in the case are indented just once. The predecessor numbers for all subtasks will then be one higher than in the text as the first line (main task) is now the Project name. The lag mentioned in the case A section is plus lag. analyze certain bodily substances and compare them widi a sample from a suspect. Forensic science consultant Richard Saferstein tells us that portions of the DNA structure are as unique to each individual as fingerprints. He writes that inside each of the 60 trillion cells in the human body are strands of genetic material called chromosomes. Arranged along the chromosomes, like beads on a thread, are nearly 100,000 genes. Genes are the fundamental unit of heredity. They instruct the body cells to make proteins drat determine everydiing from hair color to susceptibility to diseases. Each gene is actually composed of DNA specifically designed to carry out a single body function. Scientists have determined that DNA is die substance by which genetic instructions are passed from one generation to the next. (Saferstein 353-394) DNA profiling has helped investigators solve crimes and ensure that diose guilty of crimes are convicted in court. Profiling is the examination of DNA samples from a body substance or fluid to determine whether they came from a particular subject. For example, semen on a rape victim's clothing can be positively or negatively compared with a suspect's semen. police laboratories. Smaller departments may contract with large county crime labs or state police crime labs. Some departments use die services of the FBI lab. (Durose 1) Private (nongovernment) labs are taking on greater importance in the U.S. legal system. Their analyses are increasingly being introduced into criminal and civil trials, often not only as evidence but also to contradict evidence presented by .

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What unique properties of the genome do forensic scientists use to generate a DNA footprint and this footprint enough to identify a specific (one) suspect? Solution only one tenth of a single percent of DNA differs from one person to another, forensic scientist use these variable regions to generate a DNA profile of an individual using samples from blood , hair ,etc. scientist find markers in a DNA sample by designing a small pieces of DNA(probes) that will bind to a complementary DNA sequence in the sample.A series of probes bound to a DNA sample creates a distinctive pattern for individual.scientist compare these DNA profile to determine whether the suspect\'s sample matches the evidence sample.if they don\'t match the person don\'t contribute to the crime scene.If it match it means the suspect may have contributes to the evidence sample. There are variable number of tendom repeats which are used to identify the suspects, which vary in each person. RFLP(restriction fragment length polymorphism ) is a technique used in this concern as it analyze variable length of DNA fragments that results from digesting a DNA sample with a special kind of enzyme. various other techniques are also there as- PCR,STAR analysis.

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