Factors to Consider When Choosing Accounts Payable Services Providers.pptx
Blackmore, Murray
1. HIGH CONTENT SCREENING
IDENTIFIES NOVEL REGULATORS
OF CNS AXON REGENERATION
Murray Blackmore
The Miami Project to Cure Paralysis
University of Miami
6. Why can’t axons in the CNS regenerate?
Inadequate Cell Body
Response
1. Neutralize inhibitory molecules
2. Block inhibitory signaling pathways
3. Tissue / cell transplants
≠
robust regeneration
7. Many types of neurons regenerate
Zebrafish retina
PNS
Immature CNS
8. Many types of neurons regenerate
Zebrafish retina
?
PNS
Immature CNS
9. In vivo, rodent corticospinal tract (CST) neurons lose
regenerative capacity between postnatal day 3 and 16
10. In vivo, rodent corticospinal tract (CST) neurons lose
regenerative capacity between postnatal day 3 and 16
Research strategy
1. Comparative gene profiling
2. Functional screening in culture
3. Functional gene testing in vivo
11. What CST genes change in expression between P3
and P16?
Change in CST gene expression?
>1000 genes change 3x or more
12. Candidate genes are available in large libraries
Change in CST gene expression?
>1000 genes change 3x or more
Open Biosystems Library
6,200 human genes
9,800 mouse genes
About 160 96-well plates
19. Testing gene function in vivo
Gene delivery to Dorsal hemisection
CST cell bodies
1. Knock down growth suppressive genes
2. Over-express growth promoting genes
3. Overexpress/knock down in combination
29. Acknowledgements
Vance Lemmon Jeff Goldberg
Blackmore lab Darcie Moore
John Bixby
Zimei Wang Jessica Lerch-Haner
Yania Martinez Dario Motti
Andrea Johnstone Christopher Shields
Orisley Franch Tatiana Slepak Ping Zhang
Mathew Saccino Yan Shi
University of Miami
Viral Vector Core
National Institutes of Health (NINDS, NICHD) Anthony Oliva
The Buoniconti Fund
Craig Neilsen Foundation Pingping Jia
Ralph Wilson Foundation
Wallace Coulter Foundation
Walter G. Ross Foundation
DOD