Bone Presentation Final5 5 2008

CONTROL OF IN VITRO TISSUE-ENGINEERED BONE-LIKE STRUCTURES USING HUMAN MESENCHYMAL STEM CELLS AND POROUS SILK SCAFFOLDS Biomaterials 28 (2007) 1152 – 1162 S. Hoffmann, H. Hagenmuller, A. M. Koch, R. Muller, G. Vunjak-Novakovic, D. L. Kaplan, H. P. Merkle, L. Meinel Christian Britt Amy Chaibi Polliana Macedo Niyati Patel Joseph Saltzbart

Presentation Topics/ Agenda: ,[object Object],[object Object],[object Object],[object Object],[object Object]

INTRODUCTION ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bone Structure ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Mechanical Properties 2 – 12 .05 - .5 30 - 90 Trabecular 50 - 150 100 – 230 7 – 30 5 - 30 Cortical Bending Compressive Strength (MPa) Young’s Modulus (GPa) Porosity (%) Bone

Bone Function ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bone Cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Musculoskeletal Injury & Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Contribution of the Experimental Design to Solving of the Problem ,[object Object],[object Object],[object Object],[object Object]

Tissue Engineering Strategies ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

OVERALL GOAL OF EXPERIMENTAL DESIGN ,[object Object],[object Object],[object Object]

EXPERIMENTAL DESIGN ,[object Object],[object Object]

Findings Overview ,[object Object],[object Object]

Tissue-engineered bone on mixed pore scaffolds

Biochemical parameters of osteogenic differentiation of hMSC cultured on SF scaffolds

Cell proliferation and activity

hMSC on scaffolds with different pore sizes as visualized by CLSM (A–F) and histology

hMSC grown on SF scaffolds with mixed pores

Did the authors succeed? ,[object Object],[object Object],[object Object]

Competing Studies- Comparisons ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Competing Studies- Contrasts ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Medical & Clinical Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Design of a New Strategy ,[object Object],[object Object],[object Object]

Cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Scaffold ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Processing ,[object Object],[object Object],[object Object],[object Object]

Tests to Evaluate Characteristics and Efficacy ,[object Object],[object Object],Xenogeneic Cells + Animal Collagen Flow Perfusion Bioreactor Spinner Flask hMSC + SF Flow Perfusion Bioreactor Spinner Flask

Improvements Upon Current Strategies ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

References (cont’d) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

References (cont’d).. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bone Presentation Final5 5 2008

Recommandé

Recommandé

Contenu connexe

Tendances

Tendances (20)

Similaire à Bone Presentation Final5 5 2008

Similaire à Bone Presentation Final5 5 2008 (20)

Bone Presentation Final5 5 2008