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Irradiation Preservation of Foods 
By: Nooshin Noshirvani 
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the level of food loss is high (more than 40% for 
fruits & vegetables and higher for fish & meat) 
According to the United Nations, more than 30 
percent of the mortality rate world-wide is caused 
by alimentary diseases 
Some agricultural products are important 
commodities in international trade. (infestation of 
several species of insects and mites) 
The presence of parasites, some microorganisms, 
yeast and moulds are also the source of problems, 
(toxin formation)
According to Statistic Canada, 
• the number of food-borne illnesses is estimated to be more than: 
630 000 cases/year for Salmonella, 
100 000 cases/year for Staphylococcus aureus, 
19 000 cases/year for Shigella, 
16 000 cases/year for Campylobacter jejuni 
13 000 cases/year for E. coli O157: H7. 
2800 cases/year for Listeria monocytogenes, 
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Canada USA
Newest Method: 
Irradiation 
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Oldest 
Methods 
Drying 
Fermenting 
Salting 
Smoking 
Newer 
Methods 
freezing 
Canning 
Refrigeration 
Preservatives 
Pesticides 
applied to fresh, frozen or 
cooked products. 
physical safe 
environmentally 
clean 
efficient technology
• physical treatment that consists of exposing foods either prepackaged 
or in bulk to the direct action of electronic, electromagnetic rays 
• When made to bombard against materials, they can knock off an 
electron from an atom or molecule causing ionization. 
• For this reason, these are often called ionizing irradiation. 
• The X- and gamma-rays are very short wavelength radiations that 
have very high associated energy levels. 
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• Gamma Rays 
 come from the spontaneous disintegration of radionuclides. 
 cobalt-60 (1.17 and 1.33 MeV) : produced from cobalt-59 
 caesium- 137 (0.662 MeV) : a spent fuel from nuclear reactors 
 Nuclear Waste 
 Good penetration 
• Electron Beams 
Stream of high-energy electrons propelled from an electron gun (maximum 
energy 10 MeV). 
Similar to Beta Particles 
No Waste, In-line equipment 
• X-rays 
▫ beam of accelerated electrons is directed at a thin plate of gold (or other 
metal), producing a stream of X-rays exiting from the other side (5 Mev) 
▫ No Waste, In-line equipment, Good Penetration 
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Gamma Rays 
• Cobalt-60 the choice for gamma radiation source 
• produced by neutron bombardment in a nuclear reactor of the metal cobalt- 
59, then doubly encapsulated in stainless steel pencils to prevent any leakage 
during its use in an irradiator. 
• Cobalt-60 has a half-life of 5.3 years, 
• highly penetrating and can be used to treat full boxes of fresh or frozen food. 
• over 80% of the cobalt-60 available in the world market is produced in 
Canada. 
• Other producers are the Russian, Republic of China, India and South Africa. 
• Cesium 137 is the only other gamma-emitting radionuclide suitable for 
industrial processing of materials. 
• It can be obtained by reprocessing spent, or used, nuclear fuel elements and 
has a half-life of 30 years. 
• There is no supply of commercial quantities of cesium-137. 
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Electron Beams 
• Since the associated energy levels of these rays are too low to be 
practical value in preservation, they need to be accelerated (in 
cyclotrons, linear accelerators etc.) to make them acquire the 
required energy. 
• Since electrons cannot penetrate very far into food, compared with 
gamma radiation or X-rays, they can be used only for treatment of 
thin packages of food and free flowing or falling grains. 
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• chemical events as a 
result of energy 
deposition on target 
molecule 
Direct 
• radicals formed from 
indirect the radiolysis of water 
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• The international unit of measurement is the Gray (Gy). 
• One Gray represents one joule of energy absorbed per kilogram of 
irradiated product. One Gy is equivalent to 100 rad (radiation 
absorbed dose) 
• The desired dose is achieved by the time of exposure and by the 
location of the product relative to the source. 
• depend upon the mass, bulk density and thickness of the food 
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irradiation pasteurization
• The maximum dose of 10 kGy recommended by the Codex 
General Standard for Irradiated Foods is equivalent to the heat 
energy required to increase the temperature of water by 2.4ºC. 
• Irradiation is often referred to as a ‘’cold pasteurization’’ process 
as it can accomplish the same objective as thermal pasteurization 
of liquid foods, 
• For example milk, without any substantial increase in product 
temperature. 
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• 1895 W. K. Von Roentgen discovers X-rays. 
• 1896 H. Becquerel discovers radioactivity. 
• 1896 F. Minsch suggests using ionizing radiation to kill 
microorganisms in food. 
• 1903 M. Curie described 3 different types of radiation – alpha, beta and 
gamma. 
• 1904 S. C. Prescott publishes effects of ionizing radiation on bacteria. 
• 1905 U.S. and British patents are issued for the proposed use of killing 
bacteria in food with ionizing radiation. 
• 1921 B. Schwartz, a researcher at USDA, publishes studies about the 
lethal effect of X-rays on Trichinella spiralis in pork. 
• 1950s conduct research on food irradiation. 
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• 1943 Preservation of ground beef by exposure to X-rays 
demonstrated to be feasible. 
• 1950 U.S. Atomic Energy Commission begins program using 
radioisotopes for food preservation. 
• 1953 U.S. Army begins food irradiation program. 
• 1958 U.S. Federal Food, Drug and Cosmetic Act is amended, legally 
defining ionizing radiation as a food additive rather than a process. 
• USSR approves irradiation for potatoes and grain. 
• 1960 Canada approves irradiation for potatoes. 
• 1963 FDA approves irradiation for insect disinfestation of wheat and 
wheat powder. 
• 1964 FDA approves irradiation to inhibit sprouting in potatoes. 
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• 1965 FDA approves irradiation to extend the shelf life of potatoes. 
• 1968 FDA and USDA rescind approval for irradiation of bacon granted in 
1963. 
• 1976 Joint FAO/IAEA/WHO Expert Committee on the Wholesomeness 
and Safety of Food Irradiation approves several irradiated foods and 
recommends that food irradiation be classified as a physical process. 
• 1980 Joint FAO/IAEA/WHO Expert Committee concludes that any food 
irradiated up to a maximum overall average dose of 10kGy presents no 
toxicological hazard and requires no further testing. 
• 1983 FDA and Canada approve irradiation for insect disinfestation in 
spices and dry vegetable seasoning (38 commodities).
• 1985 FDA approves irradiation to control Trichinella spiralis in 
pork and to disinfest dry enzyme preparations. 
• 1986 FDA approves irradiation to delay ripening (maturation) of 
some fruits and vegetables, and to decontaminate dry or 
dehydrated enzyme preparations. 
• 1990 FDA approves irradiation to control pathogens such as 
Salmonella in fresh and frozen poultry. 
• 1997(FDA) and 1999 (USDA) Approval of irradiation to control 
pathogens in fresh and frozen red meats (beef, lamb and pork). 
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• Wheat flour – control of mold 
• White potatoes – inhibit sprouting 
• Pork – kill Trichinia parasites 
• Fruit and Vegetables – insect control; increase shelf life 
• Herbs and Spices - sterilization 
• Poultry – bacterial pathogen reduction 
• Meat – bacterial pathogen reduction 
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• Irradiation is a “cold” process, and therefore… 
▫ Little if any change in physical appearance 
 No textural or color changes as with traditional heat 
preservation 
• Possible chemical changes 
▫ Off-flavors 
▫ Tissue softening 
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Disinfestation 
Shelf Life Extension 
Decontamination 
Product Quality Improvement 
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• Commercial processing of irradiated potatoes has been carried out in 
Japan since 1973. 
• important postharvest treatments 
• A low dose of 0.15–0.50 kGy can damage insects at various stages of 
development that might be present 
• Irradiation can damage insect’s sexual viability or its capability of 
becoming an adult 
• Radiation disinfestation can facilitate trade in fresh fruits, such as 
citrus, mangoes, and papayas which often harbour insect pests of 
quarantine importance (0.2-0.7 KGy) 
• a combination treatment of low doses of gamma irradiation (0.35 kGy). 
and heat would be advantageous to cause complete killing of insects in 
dates 
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methyl bromide & 
phosphine 
irradiation 
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• A very low radiation dose of 0.15 kGy or less (0.02–0.15), inhibits 
sprouting of products such as potatoes, yams, onions, garlic, 
ginger, and chestnuts. 
• Yang et al found that the treatment of garlic bulbs with 0.15 kGy 
can inhibit sprouting and reduce weight losses during storage 
• The irradiation affects the flavor compounds of garlic. 
• delay the ripening and senescence of some tropical fruits such as 
bananas, litchis, avocados, papayas, and mangoes at 0.12–0.75 
kGy 
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• Delay Microbial development in fruits 
• Extends the shelf life of perishable products such as beef, poultry, 
and seafood by decontamination of spoilage microorganisms. 
• The shelf-life of many fruits and vegetables, meat, poultry, fish 
and seafood can be considerably prolonged by treatment with 
combinations of low-dose irradiation and refrigeration that do not 
alter flavour or texture. 
• Pseudomonas spp., are relatively sensitive to irradiation. (dose of 
2.5 kGy) applied to fresh poultry carcasses enough to eliminate 
Salmonella, and will also kill many, but not all, spoilage bacteria. 
• This will double meat shelf-life, provided it is kept below 5°C 
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• known as ‘’trichina-safe’’ 
• 0.3 Kgy for trichina & 
0.5 (Toxoplasma gondii) 
Destroy parasites 
pasteurize 
• low dose 
• 1.0–2.0 kGy 
• higher dose 
• 3.0–20 kGy 
sterilization
• Irradiation is currently the only known method to inactivate these 
pathogens in raw and frozen food. 
• Escherichia coli O157:H7, Salmonella, Campylobacter jejuni, 
Listeria monocytogenes, and Vibrio 
• Salmonella and C. jejuni are usually associated with poultry( 2.5 kGy ) 
• E. coli O157:H7 has also been linked to meat and dairy products in the 
United Kingdom, hamburger meat, apple juice and water in the USA, 
and vegetables in Japan 
• Listeria monocytogenes has been associated with dairy products, 
processed meats and other foods having a relatively long shelf-life 
under refrigeration. 
• Vibrio sppconsumption of raw mollusks. 
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• sensitivity of Pathogens to low levels of ionising radiation 
• As the irradiation dose increases more microorganisms are affected 
but a higher dose, introduce changes in sensory qualities and a 
balance must be attained between the optimum dose required 
• Eggs and egg products are often contaminated with Salmonella 
• Frozen egg and dried egg could be irradiated at doses of up to 2- 5 
kGy without quality loss and that this dose provided sufficient 
hygienic protection. 
• Seafood (shellfish & frozen shrimp) is often contaminated with 
pathogenic organisms such as Salmonella, Vibrio 
parahaemolyticus, and Shigella, Aeromonas hydrophila. dose of 
about 3 kGy
• astronauts in the NASA space shuttle programme 
• their superior quality, 
• safety 
• variety, 
• Limited commercial-scale sterilization of various ready-to-eat 
foods by high dose irradiation has been carried out in South Africa 
during the past 10 years to serve military personnel and campers, 
yachters and hikers. 
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improving product recovery 
and higher juice yield in fruits 
irradiation does not leave any 
chemical residues in foods 
Increase shelf life and 
microbiological properties
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Minimize Food Losses 
Improve Public Health 
Increase International Trade 
An Alternative to Fumigation of Food 
Increase Energy Saving
• Especially in the Third 
World, irradiation has high 
potential where in many 
cases food is spoiled during 
postharvest stage 
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Disinfestation 
sprout 
inhibition 
delayed 
ripening
• Reduction of: 
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pathogenic 
microorganisms parasites
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(i) infestation by 
insects 
(ii) infection by 
microorganisms 
(iii) their limited 
shelf life 
restricts long-distance shipments.
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ethylene 
dibromide 
ethylene 
oxide 
methyl 
bromide 
toxic nature and environmental 
impact effect
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Canning 
20,180 kJ/kg 
Refrigeration 
17,760 kJ/kg 
frozen 
storage 
46,600 kJ/kg 
refrigerated & 
irradiated 
17,860 kJ/kg
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• affects microorganisms, such as bacteria, yeasts, and molds 
• causing lesions in the genetic material of the cell, effectively 
preventing it from carrying out the biological processes necessary 
for its continued existence 
• The principal targets of irradiation are nucleic acids and membrane 
lipids
Mode of Action 
nucleic acids 
prevention of 
replication 
cell reproduction 
impossible 
membrane lipids 
functions, such 
as permeability 
membrane 
enzymes 
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Main factor of 
susceptibility 
atmosphere 
Presence of 
oxygen 
temperature Dose level Medium 
Absence of 
oxygen 
Type of 
organism 
Size 
Cell wall (Gram 
positive of 
negative) 
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Number and 
age of cells
• As a rule, the simpler the life form, the more resistant it is to the 
effects of irradiation. 
Parasites and insect pests 
• have large amounts of DNA 
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Humans Molds Bacteria viruses
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NO ACUTE 
EFFECTS 
STERILIZATION OF MICROORGANISMS 
LETHAL TO 
INSECTS 
SPROUTING 
INHIBITED 
LETHAL 
TO 
HUMANS 
1 10 102 103 104 105 106 107 
Dose 
(rad) 
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Proteins 
Effects of 
Irradiation 
Carbohydr 
ates 
Enzymes 
Vitamins Lipids
peptide linkages 
• not attacked 
sulfur linkages 
• attacked 
hydrogen bonds 
• attacked 
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Low doses : may cause molecular uncoiling, coagulation, unfolding, and even molecular cleavage 
and splitting of amino acids 
At 10 kGy radiation, overall increase in total free amino acids was observed mainly due to the rise in 
the levels of glycine, valine, methionine, lysine, isoleucine, leucine, tyrosine, and phenylalanine 
affects the functional properties of proteins 
Egg 
loss of viscosity in the white 
off-flavors in the yolk 
Milk 
off-flavors 
increase in rennet coagulation time 
reduced heat stability
• break high-molecular-weight carbohydrates into smaller units 
• softening of fruits and vegetables through breakdown of cell wall 
materials, such as pectin 
• Sugars may be hydrolyzed or oxidized 
• irradiation of wheat at 0.2–10 kGy increase in initial total reducing 
sugars and generation of bread flavor and aroma 
• Irradiation of pure carbohydrates produced degradation products, 
which have mutagenic and cytotoxic effects. 
• However, these undesirable effects were produced using very 
high dose of irradiation 
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• initiates the normal process of autoxidation of fats which gives rise 
to rancid off-flavors 
• The formation of peroxides and volatile compounds, and the 
development of rancidity and off-flavors 
• This process can be slowed by the elimination of oxygen by 
vacuum or modified atmosphere 
• The peroxide formed can also affect certain labile vitamins, such 
as vitamins E and K 
• The lipids in cereals degraded only at high doses of irradiation and 
no significant effects on iodine value, acidity, or color intensity of 
wheat flour lipids were observed 
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• The extent of vitamin C, E, and K destruction depends on the 
dosage used, 
• thiamine is very labile to irradiation. 
• The losses are low with low dose 
• Ascorbic acid in solution is quite labile to irradiation but in fruits 
and vegetables seems quite stable at low doses of treatment 
• Vitamins (antioxidant activity), such as A, B12, C, E, K, and 
thiamine, are degraded when irradiation is carried out in the 
presence of oxygen 
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• Enzymes in foods must be inactivated prior to irradiation because 
it is much more resistant to radiation than microorganisms 
• complete inactivation of enzymes requires about 5–10 times the 
dose required for the destruction of microorganisms 
• The D values of enzyme can be 50 kGy and almost four D values 
would be required for complete destruction 
• irradiated foods will be unstable during storage due to their 
susceptibility to enzymatic attack than nonirradiated foods 
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• Fruits and Vegetables (Berries, Mangoes, Carrots, Papaya, 
Strawberries) 
• Spices 
• Cereals and Grains 
• Animal Foods (Poultry, Mutton, Beef, Pork, Processed Meats, 
Fish and Fish Products) 
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 promising technology to maintain the quality of fresh fruits and vegetables because 
• it has the potential to control both spoilage and pathogenic microbes 
• physical means for pasteurization without changing the fresh state 
• at a pasteurization dose (2–5 kGy) could control post-harvest spoilage and diseases 
• undesirable symptoms are 
• tissue softening 
 partial depolymerization of cell wall polysaccharides, mainly cellulose and pectins 
 damage to cell membrane 
• enzymatic browning 
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Fungal 
diseases 
pathological 
breakdown 
insect 
infestation. 
tissue 
damage 
this technology should be used 
in combination with 
other treatments.
• irradiation with heat strong inactivation effect (1% survival) was 
obtained when irradiation plus heat (1.25 kGy 46°C, 5 min) 
• Papaya: 48.9°C for 20 min in combination delayed ripening with 
optimum dose of 0.75 kGy 
• heating and irradiation had a stronger interaction than heating and 
chilling 
• The oxidation can be minimized by irradiating in an atmosphere with 
reduced oxygen content, 
• low-dose irradiation combined with modified atmosphere is 
increasingly considered for control of microorganisms and delayed 
ripening 
• Couture and Willemot showed the synergistic action of irradiation 
combined with high carbon dioxide for control of mold development on 
strawberries. (7% oxygen and 20% carbon dioxide and 1 kGy) 
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Strawberry 1.5–2kGy 
(3 kGy) 14 days. 
Papaya & 
mango (0.25 -1 
kGy) 
Mushroom (2 - 
3kGy) two-fold
• Not all fruits and vegetables are suitable for irradiation because 
undesirable changes in colour or texture, or both, limit their 
acceptability. 
• different varieties of the same fruit or vegetable may respond 
differently to irradiation. 
• The time of harvest and the physiological state also affects the 
response of fruits and vegetables to irradiation 
• For delaying ripening in fruits it is important to irradiate them 
before ripening starts. 
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Shelf-life of strawberries can be 
extended by irradiation!!! 
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• Spices, herbs and vegetable seasonings are valued for their distinctive 
flavours, colours and aromas. 
• they are often heavily contaminated with microorganisms because of 
the environmental and processing conditions under which they are 
produced (open air drying procedures) 
• Before use in food the microbial load should be reduced. 
• Because heat treatment can cause significant loss of flavour and aroma, 
a ‘’cold process’’, such as irradiation, is ideal. 
• Until recently, most spices and herbs were fumigated, usually with 
sterilizing gases such as ethylene oxide to destroy contaminating 
microorganisms 
• the use of ethylene oxide was prohibited by an European Union (EU) 
directive in 1991 and has been banned in a number of other countries 
because it is a carcinogen. 
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• increasingly important use of irradiation for decontamination of 
spices 
• A dose of 2.5 kGy reduced the fungal and bacterial load by 2 log 
cycles, and 7.5 kGy eliminated the fungal population of ground or 
whole pepper. 
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Clostridium Staphylococcus Bacillus Aspergillus Fusarium
• Irradiation of spices on a commercial scale is practised in over 20 
countries and global production has increased significantly from 
about 5,000 tonnes in 1990 to over 60,000 tonnes in 1997. 
• In the USA alone over 30,000 tonnes of spices, herbs and dry 
ingredients were irradiated in 1997 as compared to 4,500 tonnes in 
1993. 
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• with low doses of irradiation to eliminate fungi, since some of 
these organisms can produce mycotoxins 
• 0.2–1.0 kGy are effective in controlling insect infestation in 
cereals 
• Increasing the dose to 5 kGy totally kills the spores of many fungi, 
which survive lower doses 
• loaf volume and baking quality deteriorated above 5 kGy 
irradiation irrespective of the baking formula. 
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• The irradiation is effective in preventing or delaying the microbial 
spoilage of fresh meats and poultry. 
• Early studies indicated that irradiation at doses between 0.25 and 
1kGy under aerobic conditions increased microbiological shelf 
life, but accelerated rancidity 
• In case of meats, doses up to 2.5 kGy control Salmonella, 
Campylobacter, Listeria monocytogenes, Streptococcus faecalis, 
Staphylococus aureus, and Escherichia coli in poultry and other 
meats. 
• The doses in excess of 2.5 kGy may change flavor, odor, and 
color, but these changes can be minimized by irradiating at low 
temperature or in absence of oxygen 
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oxidation of pigment 
to yield brown or gray 
discolorations by o2 
drip loss from the cut 
surface of lean, 
oxidation of meat 
lipids that causes off-flavors 
by atmospheric 
irradiation coupled with vacuum packaging 
has the potential to extend the shelf life
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• dose of 2.5–5kGy dose since this can extend shelf life at chill 
temperatures from 6 to 14 days without insignificant organoleptic 
quality change
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• The amount of nitrite required in cured meats possibly can be 
reduced by irradiation, thus the chance of nitrosamine formation 
can be lowered 
• can be reduced from normal levels of 120–150 to 20–40 mg/kg 
without loss of organoleptic quality 
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• control of pathogenic organisms and the extension of shelf life of 
fresh fish could be achieved with relatively low doses 2.5 kGy 
• Clostridium botulinum (A, B, E, and F) present in fish and fish 
products remained unaffected by the low doses of irradiation. 
• Thus, precautions during storage under 3°C and oxygen 
availability to the product need to be taken 
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• As irradiation is a cold process does not substantially raise the 
temperature of the food being processed, 
• nutrient losses are small and often significantly less than losses 
associated with other methods of preservation such as canning, 
drying and heat pasteurization. 
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• Production of gas and volatiles compounds, which may migrate into the food and cause 
off-flavors. 
• At sterilizing doses, nylon gives rise to little off-odor production, 
• in case of polyethylene, short fragmentations of the polymer are produced, which enter 
the food 
• Volatile compounds are formed in polyethylene, polyester terephthalate, and oriented 
polypropylene after irradiation dose from 5 to 50kGy. 
• Twenty-two compounds (polyester terephthalate), 40 (oriented polypropylene), and 
only acetone was identified for polyethylene, which could be a good candidate for 
irradiation of packaged food products. 
• These compounds are hydrocarbons, ketones, and aromatic compounds
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The properties of polyethylene terephthalate (PET) are well preserved 
during irradiation 
At doses of 60 kGy and higher, some damage may occur in tin-coated steel 
and aluminum containers, but at the level of sterilizing doses there should not 
be any affect 
At doses less than 20kGy, physical changes in flexible containers are 
negligible. 
High doses above 30 kGy cause brittleness in cellophanes, saran, and 
plioform, while 20 kGy or more can cause inconsequential physical changes 
in mylar, polyethane, vinyl, and polyethylene plastic films 
At strong doses of 50kGy, mechanical properties of polymers can be 
improved by cross-linking
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• the irradiation room 
• A system to transport the food into and out of the room 
• concrete shielding (1.5 - 1.8 metres thick) surrounding the 
irradiation room, which ensures that ionising radiation does not 
escape to the outside of the room. 
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• In the case of a gamma irradiator, the radionuclide source continuously 
emits radiation and when not being used to treat food must be stored in 
a water pool (usually 6 metres in depth). 
• Known as one of the best shields against radiation energy, water 
absorbs the radiation energy and protects workers from exposure if they 
must enter the room. 
• In contrast to gamma irradiators, machines producing high-energy 
electrons operate on electricity and can be switched off. 
• The transport system : conveyor or a rail system 
• In a gamma irradiator, the size of the containers in which the food is 
moved through the irradiation chamber can vary and pallets up to 1 m3 
may be used 
• with machines, the bulk or thickness of a product which can be treated 
is much less and hence there is a fundamental design difference 
between the two types of irradiator. 
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Vietna 
m 
Ukraine 
Isreal 
China 
China 
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• In 1980, it concluded that the irradiation of any food commodity up to an 
overall average dose of 10 kGy presents no toxicological hazard and requires 
no further testing. 
• in 1983, of a worldwide standard covering irradiated foods. 
• The standard was adopted by the ,(FAO) and (WHO), more than 150 
governments. 
• The Codex General Standard for food irradiation was based on the findings of 
a Joint Expert Committee on Food Irradiation (JECFI) convened by the FAO, 
WHO, and the International Atomic Energy Agency (IAEA) 
• As of August 1999, over 30 countries are irradiating food for commercial 
purposes. 
• Today, health and safety authorities in over 40 countries have approved 
irradiation of over 60 different foods, ranging from spices to grains to 
deboned chicken meat, to beef, to fruits and vegetables 
91
• In September 1997 a Study Group was jointly convened by the 
WHO, FAO and IAEA to evaluate the wholesomeness of food 
irradiated with doses above 10 kGy. 
• This Study Group concluded that there is no scientific basis for 
limiting absorbed doses to the upper level of 10 kGy as currently 
recommended by the Codex Alimentarius Commission. 
• Food irradiation technology is safe to such a degree that as long as 
the sensory qualities of food are retained and harmful 
microorganisms are destroyed, the actual amount of ionizing 
radiation applied is of secondary consideration. 
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Interest in the irradiation process is increasing because of: 
• persistently high food losses from infestation, contamination, 
spoilage; 
• Prohibition on the use of a number of chemical fumigants for 
insect and microbial control in food, 
• Effective alternative to protect food against insect damage and as a 
quarantine treatment of fresh produce. 
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• a few hundred thousand tonnes of food products and ingredients 
are irradiated worldwide. 
• This amount is small in comparison to the total volumes of 
processed foods and not many of these irradiated food products 
enter international commerce. 
• Adopting public understanding and acceptance of the process. 
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• Major Problems of Irradiation 
• Legal Aspects and Safety Issues 
• Consumers’ Attitude 
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• has a low operating cost 
• requires low energy 
But: 
high capital costs 
requires a critical minimum capacity 
threshold doses above which organoleptic changes and off-flavor 
development occur at low doses all microorganisms and their 
toxins will not be eliminated. 
Limitation in packaging material 
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• A joint FAO/IAEA/WHO Expert Committee on Food Irradiation 
(IJECFI) concluded that irradiation of food up to an overall 
average dose of 10 kGy causes no toxicological hazards and 
introduces no special nutritional or microbiological problems 
• Irradiation of food and agricultural products is currently allowed in 
about 40 countries and approximately 60 commercial irradiation 
facilities are operating in the United States 
• The most common irradiated food products for commercial use are 
spices and dry vegetable seasonings 
• recent ban on the use of ethylene oxide for food by European 
Union could increase the quantity of spices and vegetables 
seasonings processed by irradiation in the near future
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• consumer education 
• in advanced countries consumers at large are still not 
knowledgeable about food irradiation. 
• accurate information about safety, benefits, and limitations of food 
irradiation
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• NO. Neither irradiation nor any other food treatment can reverse 
the spoilage process and make bad food good 
• While irradiation can reduce or eliminate spoilage bacteria or 
pathogenic microorganisms which may be present in a spoiled 
food, it cannot improve its sensory properties , the bad appearance, 
taste or smell will remain
• all exposures of workers to radiation are prevented because the 
radiation source is shielded. 
106
• Over the past 30 years, there have been a few major accidents at 
industrial irradiation facilities that caused injury or death to 
workers because of accidental exposure to a lethal dose of 
radiation. 
• All of the accidents happened because safety systems had been 
deliberately bypassed and proper control procedures had not been 
followed. 
• None of these accidents endangered public health and 
environmental safety. 
107
• NO. 
108 
• free radicals are also formed by other food treatments, such as toasting of bread, 
frying, and freeze drying, and during normal oxidation processes in food. 
• They are generally very reactive, unstable structures, that continuously react with 
substances to form stable products. 
• Free radicals disappear by reacting with each other in the presence of liquids, 
such as saliva in the mouth. 
• Consequently, their ingestion does not create any toxicological or other 
• harmful effects.
• NO. 
• Energies from these radiation sources are too low to induce 
radioactivity in any material, including food. 
• If the acquired energy is too high, induced radioactivity in foods 
could occur upon irradiation 
109
• NO. 
• Irradiation does not make food radioactive. Everything in our 
environment, including food, contains trace amounts of 
radioactivity. 
• This means that this trace amount (about 150 to 200 
becquerels/kg) of natural radioactivity from elements such as 
potassium is unavoidable in our daily diets. 
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• سازمان انرژی اتمی ایران که مقدمات ایجاد آن از اوایل سال ۱۳۵۳ فراهم گردیده بود، با 
ً 
تصویب قانون 
سازمان انرژی اتمی در تاریخ ۱۳۵۳ عملا 
به صورت یک /۴/ ۱۶ » شخصیت « حقوقی رسمیت یافت . 
• صنایع غذایی، دامپزشکی، و دامپروری 
• در حوزه صنایع غذایی تبدیلی کشاورزی نیز برای استریل کردن محصولات )از بین بردن 
میکروبها ویروسها ، باکتریها و قارچها (می توان از کاربردهای مختلف انرژی هسته ای بهره برد. 
• تکنیک های هسته ای در حوزه دامپزشکی موارد مصرفی چون تشخیص و درمان بیما ری های 
دامی، تولید مثل دام، تغذیه دام، اصلاح نژاد، بهداشت و ایمن سازی محصولات دامی و 
خوراک دام دارد. 
• تشعشعات هسته ای کاربردهای زیادی در کشاورزی دارد که مهم ترین آنها عبارتستاز: 
• موتاسیون هسته ای ژن ها در کشاورزی 
• کنترل حشرات با تشعشعات هسته ای 
• جلوگیری از جوانه زدن سیب زمینی با اشعه گاما 
• انبار کردن میوه ها 
112
113 
سازمان انرژی 
اتمی ایران 
مرکز یزد مرکز کرج
• در کشاورزی برای افزایش عمر محصولات از مواد شیمیایی استفاده می شود و از سال 2015 به بعد تمام 
محصولاتصادراتی باید با استفاده از پرتوها استریل شوند. 
• مسئله افزایش عمر نگهداری محصولات کشاورزی نیز در کاربردهای انرژی هست های جای می گیرد و به عنوان 
مثال 131 هزار تن خرمای تولیدی فارسبه همین دلیل در نگهداری و بازار رسانی دچار مشکل است. 
• با استفاده از پرتودهی م یتوانیم خرمای این استان را بدون استفاده از محصولات شیمیایی از پاتوژن ها عاری 
نماییم. 
• پسته 11 درصد ازصادرات غیرنفتی ایران را به خود اختصاصداده است، درگیر آفلاتوکسین شده و سالانه 10 
درصد پسته ایران به این سم آلوده می شود ولی با استفاده از روشپرتودهی گاما م یتوان این مشکل را حل کرد. 
• دفع آفاتگندم و سایر دانه ها 
114
1376 • مرکز پرتو فرآیند یزد وابسته به سازمان انرژی اتمی ایران در سال با 
هدف تحقیقات و کاربرد پرتوهای الکترون و ایکس که در شتاب دهنده های 
ً 
15 مختلفتولید میشوند در کیلومتر جاده یزد - تفتدر استان یزد 
رسما شروع 
بکار کرد. 
• در نیمه اول سال 1377 شتاب دهنده الکترون پرقدرت این مرکز پرتودهی 
آزمایش ی خود را آغاز نمود. 
• پس از طی دوره آزمایش ی این شتاب دهنده از اوایل سال 1378 در جهت ارائه 
خدمات پرتودهی به صنایع و انجام پروژه های تحقیقاتی و کاربردی متعدد بکار 
گرفته شده است. 
115
116 
بخش پرتو 
فرآیند 
واحد پرتودهی 
یزد 
بخش 
آزمایشگاه و 
کنترل کیفی 
بخش 
محصولات 
انقباض 
حرارتی 
بخش پشتیبانی
• شتاب دهنده الکترون مرکز پرتو فرآیند یزد از نوع جدید ترین و پرقدرت ترین 
موجود در جهان )Rhodotron( شتاب دهنده های با چهار خروجی عمودی 
است. این شتاب دهنده از نوع رودوترون و افقی و انرژیهای 5 و 10 میلیون 
الکترون ولتمیباشد. 
• قدرت نهایی این دستگاه 100 کیلووات است و تا دو برابر قابل افزایشمیباشد 
و میتواند پرتوهای ایکسو الکترون تولید نماید. 
117
• درحال حاضر توان شتاب دهنده مرکز پرتو فرآیند یزد 100 کیلووات و انرژی 
آن حداکثر 10 مگا الکترون ولت میباشد که بالاترین انرژی مجاز شتاب دهنده 
صنعتی است ، بعبارت دیگر با این سیستم میتوان در هر ساعت حدود 10 
مترمکعب محصولاتی مانند لوازم پزشکی را استریل نمود. 
118
1. پرتودهی مواد پلیمری : 
2. پرتودهی محصولات پزشکی یکبار مصرف : 
3. پرتودهی مواد غذایی : 
4. کنترل کیفی مواد پلیمری : 
5. میکروبیولوژی : 
6. انجام پروژه های تحقیقاتی : 
7. دزیمتری 
119
120 
محصولات غذایی مورد تیمار: محصولات خشک شامل حبوبات، ادویه ها و  
سبزیجات 
محصولات غیر غذایی: وسایل بهداشتی، پودر بچه، وسایل استریل جراحی، نخ  
های بخیه
• خرما: کاهش بار میکروبی و آفات 
• انار : مقابله با آفت گلوگاه انار 
• سیب: مقابله با کپک پنی سلیوم 
• میگو: کنترل ویبریو 
• گل های زینتی: از بین بردن آفات و شته، رنگ و شمایل متفاوت 
• تولید آنزیمهای گلوکاناز، پکتیناز از قارچها 
• افزایش تولید اسید گلوتامیک از کرنی باکتریوم گلوتامیکوم 
• تولید اسید آمینه لیزین : کاربرد در مواد غذایی و دامی 
• گیاهان دارویی: تولید ترکیبات معطر جدید 
• تولید ارقام جدید حاصل از پرتودهی با استفاده از جهش و موتاسیون محصولات کشاورزی یکی از 
مباحثی بوده که برای اجرای آن بستر لازم توسط محققان مرکز تحقیقات جهاد کشاورزی فراهم شده 
است. 
• افزایش توان تولید آستاگزانتین توسط مخمر فافیا که در درمان آب مروارید، آب سیاه و سرطان پستان نقش 
دارد 
121
122 
ارزیابی 2 سال پروژه 
یافتن دز 
مناسب 
بهینه سازی 
بررس ی 
مشکلات 
رادیکال های 
آزاد 
ارزیابی حس ی
• احتمال کاهش رنگ میوه ها و سبزی ها ؛ کاهش رنگ قرمز انار و اثر آنتی 
اکسیدانی آن 
• ایجاد موتانتهای جدید باکتری و غیر قابل شناسایی بودن آنها 
• کامل نبودن زیر ساخت ها، 
• کاهش چشمه رادیوایزوتوپ ها و طولانی شدن مدت زمان پرتودهی 
• کمبود منابع مالی و تحریم های پیش رو از جمله چالش های این فناوری )تکمیل 
مرکز پرتودهی گامای خاورمیانه 110 میلیارد ریال اعتبارنیاز دارد( 
123
• سامانه پرتودهی چند منظوره گاما در شهرکرد در حال ساخت 
• ساخت پرتودهی گاما در شیراز 
• پیشنهاد سازمان انرژی اتمی مبنی بر ساخت سامانه پرتودهی در بناب 
124
• بیشترین کاربرد در ایران: 
استرلیزاسیون سرد ادویه ها  
دفع آفات حبوبات  
• آئین كار پرتودهي ادويه 3102 
• تجهیزات پرتودهي مواد غذايي – آئین كار كاربرد 8033 
125
• ماکیان: 7 کیلوگری 
• حبوبات: 1 کیلوگری 
• ادویه ها و چاشنی ها: 
کنترل آفت: 1 کیلوگری  
کاهش بار میکروبی: 10 کیلوگری  
• توت فرنگی: 3 کیلوگری 
2 کیلوگری / • شیلات: 2 
• گندم: 1 کیلوگری 
• سیب زمینی و پیاز: 1 کیلوگری 
• خرما: 1 کیلوگری 
126
Are irradiated foods safe? 
YES! 
Radiation doses are never large enough to cause 
nuclear changes that would cause the food material 
to become radioactive. 
127
So, now would you eat an irradiated food 
product? 
Why 
Or 
Why not? 
128
129

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Irradiation preservation of foods

  • 1. Irradiation Preservation of Foods By: Nooshin Noshirvani 1
  • 2. 2
  • 3. 3 the level of food loss is high (more than 40% for fruits & vegetables and higher for fish & meat) According to the United Nations, more than 30 percent of the mortality rate world-wide is caused by alimentary diseases Some agricultural products are important commodities in international trade. (infestation of several species of insects and mites) The presence of parasites, some microorganisms, yeast and moulds are also the source of problems, (toxin formation)
  • 4. According to Statistic Canada, • the number of food-borne illnesses is estimated to be more than: 630 000 cases/year for Salmonella, 100 000 cases/year for Staphylococcus aureus, 19 000 cases/year for Shigella, 16 000 cases/year for Campylobacter jejuni 13 000 cases/year for E. coli O157: H7. 2800 cases/year for Listeria monocytogenes, 4 Canada USA
  • 5. Newest Method: Irradiation 5 Oldest Methods Drying Fermenting Salting Smoking Newer Methods freezing Canning Refrigeration Preservatives Pesticides applied to fresh, frozen or cooked products. physical safe environmentally clean efficient technology
  • 6. • physical treatment that consists of exposing foods either prepackaged or in bulk to the direct action of electronic, electromagnetic rays • When made to bombard against materials, they can knock off an electron from an atom or molecule causing ionization. • For this reason, these are often called ionizing irradiation. • The X- and gamma-rays are very short wavelength radiations that have very high associated energy levels. 6
  • 7. 7
  • 8. • Gamma Rays  come from the spontaneous disintegration of radionuclides.  cobalt-60 (1.17 and 1.33 MeV) : produced from cobalt-59  caesium- 137 (0.662 MeV) : a spent fuel from nuclear reactors  Nuclear Waste  Good penetration • Electron Beams Stream of high-energy electrons propelled from an electron gun (maximum energy 10 MeV). Similar to Beta Particles No Waste, In-line equipment • X-rays ▫ beam of accelerated electrons is directed at a thin plate of gold (or other metal), producing a stream of X-rays exiting from the other side (5 Mev) ▫ No Waste, In-line equipment, Good Penetration 8
  • 9. 9
  • 10. Gamma Rays • Cobalt-60 the choice for gamma radiation source • produced by neutron bombardment in a nuclear reactor of the metal cobalt- 59, then doubly encapsulated in stainless steel pencils to prevent any leakage during its use in an irradiator. • Cobalt-60 has a half-life of 5.3 years, • highly penetrating and can be used to treat full boxes of fresh or frozen food. • over 80% of the cobalt-60 available in the world market is produced in Canada. • Other producers are the Russian, Republic of China, India and South Africa. • Cesium 137 is the only other gamma-emitting radionuclide suitable for industrial processing of materials. • It can be obtained by reprocessing spent, or used, nuclear fuel elements and has a half-life of 30 years. • There is no supply of commercial quantities of cesium-137. 10
  • 11. Electron Beams • Since the associated energy levels of these rays are too low to be practical value in preservation, they need to be accelerated (in cyclotrons, linear accelerators etc.) to make them acquire the required energy. • Since electrons cannot penetrate very far into food, compared with gamma radiation or X-rays, they can be used only for treatment of thin packages of food and free flowing or falling grains. 11
  • 12. • chemical events as a result of energy deposition on target molecule Direct • radicals formed from indirect the radiolysis of water 12
  • 13. • The international unit of measurement is the Gray (Gy). • One Gray represents one joule of energy absorbed per kilogram of irradiated product. One Gy is equivalent to 100 rad (radiation absorbed dose) • The desired dose is achieved by the time of exposure and by the location of the product relative to the source. • depend upon the mass, bulk density and thickness of the food 13
  • 14. 14
  • 15. 15
  • 16. 16
  • 18. • The maximum dose of 10 kGy recommended by the Codex General Standard for Irradiated Foods is equivalent to the heat energy required to increase the temperature of water by 2.4ºC. • Irradiation is often referred to as a ‘’cold pasteurization’’ process as it can accomplish the same objective as thermal pasteurization of liquid foods, • For example milk, without any substantial increase in product temperature. 18
  • 19. • 1895 W. K. Von Roentgen discovers X-rays. • 1896 H. Becquerel discovers radioactivity. • 1896 F. Minsch suggests using ionizing radiation to kill microorganisms in food. • 1903 M. Curie described 3 different types of radiation – alpha, beta and gamma. • 1904 S. C. Prescott publishes effects of ionizing radiation on bacteria. • 1905 U.S. and British patents are issued for the proposed use of killing bacteria in food with ionizing radiation. • 1921 B. Schwartz, a researcher at USDA, publishes studies about the lethal effect of X-rays on Trichinella spiralis in pork. • 1950s conduct research on food irradiation. 19
  • 20. • 1943 Preservation of ground beef by exposure to X-rays demonstrated to be feasible. • 1950 U.S. Atomic Energy Commission begins program using radioisotopes for food preservation. • 1953 U.S. Army begins food irradiation program. • 1958 U.S. Federal Food, Drug and Cosmetic Act is amended, legally defining ionizing radiation as a food additive rather than a process. • USSR approves irradiation for potatoes and grain. • 1960 Canada approves irradiation for potatoes. • 1963 FDA approves irradiation for insect disinfestation of wheat and wheat powder. • 1964 FDA approves irradiation to inhibit sprouting in potatoes. 20
  • 21. 21 • 1965 FDA approves irradiation to extend the shelf life of potatoes. • 1968 FDA and USDA rescind approval for irradiation of bacon granted in 1963. • 1976 Joint FAO/IAEA/WHO Expert Committee on the Wholesomeness and Safety of Food Irradiation approves several irradiated foods and recommends that food irradiation be classified as a physical process. • 1980 Joint FAO/IAEA/WHO Expert Committee concludes that any food irradiated up to a maximum overall average dose of 10kGy presents no toxicological hazard and requires no further testing. • 1983 FDA and Canada approve irradiation for insect disinfestation in spices and dry vegetable seasoning (38 commodities).
  • 22. • 1985 FDA approves irradiation to control Trichinella spiralis in pork and to disinfest dry enzyme preparations. • 1986 FDA approves irradiation to delay ripening (maturation) of some fruits and vegetables, and to decontaminate dry or dehydrated enzyme preparations. • 1990 FDA approves irradiation to control pathogens such as Salmonella in fresh and frozen poultry. • 1997(FDA) and 1999 (USDA) Approval of irradiation to control pathogens in fresh and frozen red meats (beef, lamb and pork). 22
  • 23. • Wheat flour – control of mold • White potatoes – inhibit sprouting • Pork – kill Trichinia parasites • Fruit and Vegetables – insect control; increase shelf life • Herbs and Spices - sterilization • Poultry – bacterial pathogen reduction • Meat – bacterial pathogen reduction 23
  • 24. • Irradiation is a “cold” process, and therefore… ▫ Little if any change in physical appearance  No textural or color changes as with traditional heat preservation • Possible chemical changes ▫ Off-flavors ▫ Tissue softening 24
  • 25. Disinfestation Shelf Life Extension Decontamination Product Quality Improvement 25
  • 26. 26
  • 27. • Commercial processing of irradiated potatoes has been carried out in Japan since 1973. • important postharvest treatments • A low dose of 0.15–0.50 kGy can damage insects at various stages of development that might be present • Irradiation can damage insect’s sexual viability or its capability of becoming an adult • Radiation disinfestation can facilitate trade in fresh fruits, such as citrus, mangoes, and papayas which often harbour insect pests of quarantine importance (0.2-0.7 KGy) • a combination treatment of low doses of gamma irradiation (0.35 kGy). and heat would be advantageous to cause complete killing of insects in dates 27
  • 28. methyl bromide & phosphine irradiation 28
  • 29. 29
  • 30. • A very low radiation dose of 0.15 kGy or less (0.02–0.15), inhibits sprouting of products such as potatoes, yams, onions, garlic, ginger, and chestnuts. • Yang et al found that the treatment of garlic bulbs with 0.15 kGy can inhibit sprouting and reduce weight losses during storage • The irradiation affects the flavor compounds of garlic. • delay the ripening and senescence of some tropical fruits such as bananas, litchis, avocados, papayas, and mangoes at 0.12–0.75 kGy 30
  • 31. • Delay Microbial development in fruits • Extends the shelf life of perishable products such as beef, poultry, and seafood by decontamination of spoilage microorganisms. • The shelf-life of many fruits and vegetables, meat, poultry, fish and seafood can be considerably prolonged by treatment with combinations of low-dose irradiation and refrigeration that do not alter flavour or texture. • Pseudomonas spp., are relatively sensitive to irradiation. (dose of 2.5 kGy) applied to fresh poultry carcasses enough to eliminate Salmonella, and will also kill many, but not all, spoilage bacteria. • This will double meat shelf-life, provided it is kept below 5°C 31
  • 32. 32
  • 33. 33 • known as ‘’trichina-safe’’ • 0.3 Kgy for trichina & 0.5 (Toxoplasma gondii) Destroy parasites pasteurize • low dose • 1.0–2.0 kGy • higher dose • 3.0–20 kGy sterilization
  • 34. • Irradiation is currently the only known method to inactivate these pathogens in raw and frozen food. • Escherichia coli O157:H7, Salmonella, Campylobacter jejuni, Listeria monocytogenes, and Vibrio • Salmonella and C. jejuni are usually associated with poultry( 2.5 kGy ) • E. coli O157:H7 has also been linked to meat and dairy products in the United Kingdom, hamburger meat, apple juice and water in the USA, and vegetables in Japan • Listeria monocytogenes has been associated with dairy products, processed meats and other foods having a relatively long shelf-life under refrigeration. • Vibrio sppconsumption of raw mollusks. 34
  • 35. 35 • sensitivity of Pathogens to low levels of ionising radiation • As the irradiation dose increases more microorganisms are affected but a higher dose, introduce changes in sensory qualities and a balance must be attained between the optimum dose required • Eggs and egg products are often contaminated with Salmonella • Frozen egg and dried egg could be irradiated at doses of up to 2- 5 kGy without quality loss and that this dose provided sufficient hygienic protection. • Seafood (shellfish & frozen shrimp) is often contaminated with pathogenic organisms such as Salmonella, Vibrio parahaemolyticus, and Shigella, Aeromonas hydrophila. dose of about 3 kGy
  • 36. • astronauts in the NASA space shuttle programme • their superior quality, • safety • variety, • Limited commercial-scale sterilization of various ready-to-eat foods by high dose irradiation has been carried out in South Africa during the past 10 years to serve military personnel and campers, yachters and hikers. 36
  • 37. 37 improving product recovery and higher juice yield in fruits irradiation does not leave any chemical residues in foods Increase shelf life and microbiological properties
  • 38. 38 Minimize Food Losses Improve Public Health Increase International Trade An Alternative to Fumigation of Food Increase Energy Saving
  • 39. • Especially in the Third World, irradiation has high potential where in many cases food is spoiled during postharvest stage 39 Disinfestation sprout inhibition delayed ripening
  • 40. • Reduction of: 40 pathogenic microorganisms parasites
  • 41. 41 (i) infestation by insects (ii) infection by microorganisms (iii) their limited shelf life restricts long-distance shipments.
  • 42. 42 ethylene dibromide ethylene oxide methyl bromide toxic nature and environmental impact effect
  • 43. 43 Canning 20,180 kJ/kg Refrigeration 17,760 kJ/kg frozen storage 46,600 kJ/kg refrigerated & irradiated 17,860 kJ/kg
  • 44. 44 • affects microorganisms, such as bacteria, yeasts, and molds • causing lesions in the genetic material of the cell, effectively preventing it from carrying out the biological processes necessary for its continued existence • The principal targets of irradiation are nucleic acids and membrane lipids
  • 45. Mode of Action nucleic acids prevention of replication cell reproduction impossible membrane lipids functions, such as permeability membrane enzymes 45
  • 46. Main factor of susceptibility atmosphere Presence of oxygen temperature Dose level Medium Absence of oxygen Type of organism Size Cell wall (Gram positive of negative) 46 Number and age of cells
  • 47. • As a rule, the simpler the life form, the more resistant it is to the effects of irradiation. Parasites and insect pests • have large amounts of DNA 47 Humans Molds Bacteria viruses
  • 48. 48
  • 49. NO ACUTE EFFECTS STERILIZATION OF MICROORGANISMS LETHAL TO INSECTS SPROUTING INHIBITED LETHAL TO HUMANS 1 10 102 103 104 105 106 107 Dose (rad) 49
  • 50. 50 Proteins Effects of Irradiation Carbohydr ates Enzymes Vitamins Lipids
  • 51. peptide linkages • not attacked sulfur linkages • attacked hydrogen bonds • attacked 51 Low doses : may cause molecular uncoiling, coagulation, unfolding, and even molecular cleavage and splitting of amino acids At 10 kGy radiation, overall increase in total free amino acids was observed mainly due to the rise in the levels of glycine, valine, methionine, lysine, isoleucine, leucine, tyrosine, and phenylalanine affects the functional properties of proteins Egg loss of viscosity in the white off-flavors in the yolk Milk off-flavors increase in rennet coagulation time reduced heat stability
  • 52. • break high-molecular-weight carbohydrates into smaller units • softening of fruits and vegetables through breakdown of cell wall materials, such as pectin • Sugars may be hydrolyzed or oxidized • irradiation of wheat at 0.2–10 kGy increase in initial total reducing sugars and generation of bread flavor and aroma • Irradiation of pure carbohydrates produced degradation products, which have mutagenic and cytotoxic effects. • However, these undesirable effects were produced using very high dose of irradiation 52
  • 53. • initiates the normal process of autoxidation of fats which gives rise to rancid off-flavors • The formation of peroxides and volatile compounds, and the development of rancidity and off-flavors • This process can be slowed by the elimination of oxygen by vacuum or modified atmosphere • The peroxide formed can also affect certain labile vitamins, such as vitamins E and K • The lipids in cereals degraded only at high doses of irradiation and no significant effects on iodine value, acidity, or color intensity of wheat flour lipids were observed 53
  • 54. • The extent of vitamin C, E, and K destruction depends on the dosage used, • thiamine is very labile to irradiation. • The losses are low with low dose • Ascorbic acid in solution is quite labile to irradiation but in fruits and vegetables seems quite stable at low doses of treatment • Vitamins (antioxidant activity), such as A, B12, C, E, K, and thiamine, are degraded when irradiation is carried out in the presence of oxygen 54
  • 55. • Enzymes in foods must be inactivated prior to irradiation because it is much more resistant to radiation than microorganisms • complete inactivation of enzymes requires about 5–10 times the dose required for the destruction of microorganisms • The D values of enzyme can be 50 kGy and almost four D values would be required for complete destruction • irradiated foods will be unstable during storage due to their susceptibility to enzymatic attack than nonirradiated foods 55
  • 56. • Fruits and Vegetables (Berries, Mangoes, Carrots, Papaya, Strawberries) • Spices • Cereals and Grains • Animal Foods (Poultry, Mutton, Beef, Pork, Processed Meats, Fish and Fish Products) 56
  • 57.  promising technology to maintain the quality of fresh fruits and vegetables because • it has the potential to control both spoilage and pathogenic microbes • physical means for pasteurization without changing the fresh state • at a pasteurization dose (2–5 kGy) could control post-harvest spoilage and diseases • undesirable symptoms are • tissue softening  partial depolymerization of cell wall polysaccharides, mainly cellulose and pectins  damage to cell membrane • enzymatic browning 57 Fungal diseases pathological breakdown insect infestation. tissue damage this technology should be used in combination with other treatments.
  • 58. • irradiation with heat strong inactivation effect (1% survival) was obtained when irradiation plus heat (1.25 kGy 46°C, 5 min) • Papaya: 48.9°C for 20 min in combination delayed ripening with optimum dose of 0.75 kGy • heating and irradiation had a stronger interaction than heating and chilling • The oxidation can be minimized by irradiating in an atmosphere with reduced oxygen content, • low-dose irradiation combined with modified atmosphere is increasingly considered for control of microorganisms and delayed ripening • Couture and Willemot showed the synergistic action of irradiation combined with high carbon dioxide for control of mold development on strawberries. (7% oxygen and 20% carbon dioxide and 1 kGy) 58
  • 59. 59 Strawberry 1.5–2kGy (3 kGy) 14 days. Papaya & mango (0.25 -1 kGy) Mushroom (2 - 3kGy) two-fold
  • 60. • Not all fruits and vegetables are suitable for irradiation because undesirable changes in colour or texture, or both, limit their acceptability. • different varieties of the same fruit or vegetable may respond differently to irradiation. • The time of harvest and the physiological state also affects the response of fruits and vegetables to irradiation • For delaying ripening in fruits it is important to irradiate them before ripening starts. 60
  • 61. Shelf-life of strawberries can be extended by irradiation!!! 61
  • 62. 62
  • 63. • Spices, herbs and vegetable seasonings are valued for their distinctive flavours, colours and aromas. • they are often heavily contaminated with microorganisms because of the environmental and processing conditions under which they are produced (open air drying procedures) • Before use in food the microbial load should be reduced. • Because heat treatment can cause significant loss of flavour and aroma, a ‘’cold process’’, such as irradiation, is ideal. • Until recently, most spices and herbs were fumigated, usually with sterilizing gases such as ethylene oxide to destroy contaminating microorganisms • the use of ethylene oxide was prohibited by an European Union (EU) directive in 1991 and has been banned in a number of other countries because it is a carcinogen. 63
  • 64. • increasingly important use of irradiation for decontamination of spices • A dose of 2.5 kGy reduced the fungal and bacterial load by 2 log cycles, and 7.5 kGy eliminated the fungal population of ground or whole pepper. 64 Clostridium Staphylococcus Bacillus Aspergillus Fusarium
  • 65. • Irradiation of spices on a commercial scale is practised in over 20 countries and global production has increased significantly from about 5,000 tonnes in 1990 to over 60,000 tonnes in 1997. • In the USA alone over 30,000 tonnes of spices, herbs and dry ingredients were irradiated in 1997 as compared to 4,500 tonnes in 1993. 65
  • 66. • with low doses of irradiation to eliminate fungi, since some of these organisms can produce mycotoxins • 0.2–1.0 kGy are effective in controlling insect infestation in cereals • Increasing the dose to 5 kGy totally kills the spores of many fungi, which survive lower doses • loaf volume and baking quality deteriorated above 5 kGy irradiation irrespective of the baking formula. 66
  • 67. • The irradiation is effective in preventing or delaying the microbial spoilage of fresh meats and poultry. • Early studies indicated that irradiation at doses between 0.25 and 1kGy under aerobic conditions increased microbiological shelf life, but accelerated rancidity • In case of meats, doses up to 2.5 kGy control Salmonella, Campylobacter, Listeria monocytogenes, Streptococcus faecalis, Staphylococus aureus, and Escherichia coli in poultry and other meats. • The doses in excess of 2.5 kGy may change flavor, odor, and color, but these changes can be minimized by irradiating at low temperature or in absence of oxygen 67
  • 68. 68 oxidation of pigment to yield brown or gray discolorations by o2 drip loss from the cut surface of lean, oxidation of meat lipids that causes off-flavors by atmospheric irradiation coupled with vacuum packaging has the potential to extend the shelf life
  • 69. 69 • dose of 2.5–5kGy dose since this can extend shelf life at chill temperatures from 6 to 14 days without insignificant organoleptic quality change
  • 70. 70
  • 71. • The amount of nitrite required in cured meats possibly can be reduced by irradiation, thus the chance of nitrosamine formation can be lowered • can be reduced from normal levels of 120–150 to 20–40 mg/kg without loss of organoleptic quality 71
  • 72. 72
  • 73. • control of pathogenic organisms and the extension of shelf life of fresh fish could be achieved with relatively low doses 2.5 kGy • Clostridium botulinum (A, B, E, and F) present in fish and fish products remained unaffected by the low doses of irradiation. • Thus, precautions during storage under 3°C and oxygen availability to the product need to be taken 73
  • 74. 74
  • 75. • As irradiation is a cold process does not substantially raise the temperature of the food being processed, • nutrient losses are small and often significantly less than losses associated with other methods of preservation such as canning, drying and heat pasteurization. 75
  • 76. 76
  • 77. 77
  • 78. 78 • Production of gas and volatiles compounds, which may migrate into the food and cause off-flavors. • At sterilizing doses, nylon gives rise to little off-odor production, • in case of polyethylene, short fragmentations of the polymer are produced, which enter the food • Volatile compounds are formed in polyethylene, polyester terephthalate, and oriented polypropylene after irradiation dose from 5 to 50kGy. • Twenty-two compounds (polyester terephthalate), 40 (oriented polypropylene), and only acetone was identified for polyethylene, which could be a good candidate for irradiation of packaged food products. • These compounds are hydrocarbons, ketones, and aromatic compounds
  • 79. 79 The properties of polyethylene terephthalate (PET) are well preserved during irradiation At doses of 60 kGy and higher, some damage may occur in tin-coated steel and aluminum containers, but at the level of sterilizing doses there should not be any affect At doses less than 20kGy, physical changes in flexible containers are negligible. High doses above 30 kGy cause brittleness in cellophanes, saran, and plioform, while 20 kGy or more can cause inconsequential physical changes in mylar, polyethane, vinyl, and polyethylene plastic films At strong doses of 50kGy, mechanical properties of polymers can be improved by cross-linking
  • 80. 80
  • 81. • the irradiation room • A system to transport the food into and out of the room • concrete shielding (1.5 - 1.8 metres thick) surrounding the irradiation room, which ensures that ionising radiation does not escape to the outside of the room. 81
  • 82. • In the case of a gamma irradiator, the radionuclide source continuously emits radiation and when not being used to treat food must be stored in a water pool (usually 6 metres in depth). • Known as one of the best shields against radiation energy, water absorbs the radiation energy and protects workers from exposure if they must enter the room. • In contrast to gamma irradiators, machines producing high-energy electrons operate on electricity and can be switched off. • The transport system : conveyor or a rail system • In a gamma irradiator, the size of the containers in which the food is moved through the irradiation chamber can vary and pallets up to 1 m3 may be used • with machines, the bulk or thickness of a product which can be treated is much less and hence there is a fundamental design difference between the two types of irradiator. 82
  • 83. 83
  • 84. 84
  • 85. 85
  • 86. 86
  • 87. 87
  • 88. 88
  • 89. 89
  • 90. Vietna m Ukraine Isreal China China 90
  • 91. • In 1980, it concluded that the irradiation of any food commodity up to an overall average dose of 10 kGy presents no toxicological hazard and requires no further testing. • in 1983, of a worldwide standard covering irradiated foods. • The standard was adopted by the ,(FAO) and (WHO), more than 150 governments. • The Codex General Standard for food irradiation was based on the findings of a Joint Expert Committee on Food Irradiation (JECFI) convened by the FAO, WHO, and the International Atomic Energy Agency (IAEA) • As of August 1999, over 30 countries are irradiating food for commercial purposes. • Today, health and safety authorities in over 40 countries have approved irradiation of over 60 different foods, ranging from spices to grains to deboned chicken meat, to beef, to fruits and vegetables 91
  • 92. • In September 1997 a Study Group was jointly convened by the WHO, FAO and IAEA to evaluate the wholesomeness of food irradiated with doses above 10 kGy. • This Study Group concluded that there is no scientific basis for limiting absorbed doses to the upper level of 10 kGy as currently recommended by the Codex Alimentarius Commission. • Food irradiation technology is safe to such a degree that as long as the sensory qualities of food are retained and harmful microorganisms are destroyed, the actual amount of ionizing radiation applied is of secondary consideration. 92
  • 93. Interest in the irradiation process is increasing because of: • persistently high food losses from infestation, contamination, spoilage; • Prohibition on the use of a number of chemical fumigants for insect and microbial control in food, • Effective alternative to protect food against insect damage and as a quarantine treatment of fresh produce. 93
  • 94. 94
  • 95. • a few hundred thousand tonnes of food products and ingredients are irradiated worldwide. • This amount is small in comparison to the total volumes of processed foods and not many of these irradiated food products enter international commerce. • Adopting public understanding and acceptance of the process. 95
  • 96. • Major Problems of Irradiation • Legal Aspects and Safety Issues • Consumers’ Attitude 96
  • 97. • has a low operating cost • requires low energy But: high capital costs requires a critical minimum capacity threshold doses above which organoleptic changes and off-flavor development occur at low doses all microorganisms and their toxins will not be eliminated. Limitation in packaging material 97
  • 98. 98 • A joint FAO/IAEA/WHO Expert Committee on Food Irradiation (IJECFI) concluded that irradiation of food up to an overall average dose of 10 kGy causes no toxicological hazards and introduces no special nutritional or microbiological problems • Irradiation of food and agricultural products is currently allowed in about 40 countries and approximately 60 commercial irradiation facilities are operating in the United States • The most common irradiated food products for commercial use are spices and dry vegetable seasonings • recent ban on the use of ethylene oxide for food by European Union could increase the quantity of spices and vegetables seasonings processed by irradiation in the near future
  • 99. 99
  • 100. 100
  • 101. 101
  • 102. 102
  • 103. 103 • consumer education • in advanced countries consumers at large are still not knowledgeable about food irradiation. • accurate information about safety, benefits, and limitations of food irradiation
  • 104. 104
  • 105. 105 • NO. Neither irradiation nor any other food treatment can reverse the spoilage process and make bad food good • While irradiation can reduce or eliminate spoilage bacteria or pathogenic microorganisms which may be present in a spoiled food, it cannot improve its sensory properties , the bad appearance, taste or smell will remain
  • 106. • all exposures of workers to radiation are prevented because the radiation source is shielded. 106
  • 107. • Over the past 30 years, there have been a few major accidents at industrial irradiation facilities that caused injury or death to workers because of accidental exposure to a lethal dose of radiation. • All of the accidents happened because safety systems had been deliberately bypassed and proper control procedures had not been followed. • None of these accidents endangered public health and environmental safety. 107
  • 108. • NO. 108 • free radicals are also formed by other food treatments, such as toasting of bread, frying, and freeze drying, and during normal oxidation processes in food. • They are generally very reactive, unstable structures, that continuously react with substances to form stable products. • Free radicals disappear by reacting with each other in the presence of liquids, such as saliva in the mouth. • Consequently, their ingestion does not create any toxicological or other • harmful effects.
  • 109. • NO. • Energies from these radiation sources are too low to induce radioactivity in any material, including food. • If the acquired energy is too high, induced radioactivity in foods could occur upon irradiation 109
  • 110. • NO. • Irradiation does not make food radioactive. Everything in our environment, including food, contains trace amounts of radioactivity. • This means that this trace amount (about 150 to 200 becquerels/kg) of natural radioactivity from elements such as potassium is unavoidable in our daily diets. 110
  • 111. 111
  • 112. • سازمان انرژی اتمی ایران که مقدمات ایجاد آن از اوایل سال ۱۳۵۳ فراهم گردیده بود، با ً تصویب قانون سازمان انرژی اتمی در تاریخ ۱۳۵۳ عملا به صورت یک /۴/ ۱۶ » شخصیت « حقوقی رسمیت یافت . • صنایع غذایی، دامپزشکی، و دامپروری • در حوزه صنایع غذایی تبدیلی کشاورزی نیز برای استریل کردن محصولات )از بین بردن میکروبها ویروسها ، باکتریها و قارچها (می توان از کاربردهای مختلف انرژی هسته ای بهره برد. • تکنیک های هسته ای در حوزه دامپزشکی موارد مصرفی چون تشخیص و درمان بیما ری های دامی، تولید مثل دام، تغذیه دام، اصلاح نژاد، بهداشت و ایمن سازی محصولات دامی و خوراک دام دارد. • تشعشعات هسته ای کاربردهای زیادی در کشاورزی دارد که مهم ترین آنها عبارتستاز: • موتاسیون هسته ای ژن ها در کشاورزی • کنترل حشرات با تشعشعات هسته ای • جلوگیری از جوانه زدن سیب زمینی با اشعه گاما • انبار کردن میوه ها 112
  • 113. 113 سازمان انرژی اتمی ایران مرکز یزد مرکز کرج
  • 114. • در کشاورزی برای افزایش عمر محصولات از مواد شیمیایی استفاده می شود و از سال 2015 به بعد تمام محصولاتصادراتی باید با استفاده از پرتوها استریل شوند. • مسئله افزایش عمر نگهداری محصولات کشاورزی نیز در کاربردهای انرژی هست های جای می گیرد و به عنوان مثال 131 هزار تن خرمای تولیدی فارسبه همین دلیل در نگهداری و بازار رسانی دچار مشکل است. • با استفاده از پرتودهی م یتوانیم خرمای این استان را بدون استفاده از محصولات شیمیایی از پاتوژن ها عاری نماییم. • پسته 11 درصد ازصادرات غیرنفتی ایران را به خود اختصاصداده است، درگیر آفلاتوکسین شده و سالانه 10 درصد پسته ایران به این سم آلوده می شود ولی با استفاده از روشپرتودهی گاما م یتوان این مشکل را حل کرد. • دفع آفاتگندم و سایر دانه ها 114
  • 115. 1376 • مرکز پرتو فرآیند یزد وابسته به سازمان انرژی اتمی ایران در سال با هدف تحقیقات و کاربرد پرتوهای الکترون و ایکس که در شتاب دهنده های ً 15 مختلفتولید میشوند در کیلومتر جاده یزد - تفتدر استان یزد رسما شروع بکار کرد. • در نیمه اول سال 1377 شتاب دهنده الکترون پرقدرت این مرکز پرتودهی آزمایش ی خود را آغاز نمود. • پس از طی دوره آزمایش ی این شتاب دهنده از اوایل سال 1378 در جهت ارائه خدمات پرتودهی به صنایع و انجام پروژه های تحقیقاتی و کاربردی متعدد بکار گرفته شده است. 115
  • 116. 116 بخش پرتو فرآیند واحد پرتودهی یزد بخش آزمایشگاه و کنترل کیفی بخش محصولات انقباض حرارتی بخش پشتیبانی
  • 117. • شتاب دهنده الکترون مرکز پرتو فرآیند یزد از نوع جدید ترین و پرقدرت ترین موجود در جهان )Rhodotron( شتاب دهنده های با چهار خروجی عمودی است. این شتاب دهنده از نوع رودوترون و افقی و انرژیهای 5 و 10 میلیون الکترون ولتمیباشد. • قدرت نهایی این دستگاه 100 کیلووات است و تا دو برابر قابل افزایشمیباشد و میتواند پرتوهای ایکسو الکترون تولید نماید. 117
  • 118. • درحال حاضر توان شتاب دهنده مرکز پرتو فرآیند یزد 100 کیلووات و انرژی آن حداکثر 10 مگا الکترون ولت میباشد که بالاترین انرژی مجاز شتاب دهنده صنعتی است ، بعبارت دیگر با این سیستم میتوان در هر ساعت حدود 10 مترمکعب محصولاتی مانند لوازم پزشکی را استریل نمود. 118
  • 119. 1. پرتودهی مواد پلیمری : 2. پرتودهی محصولات پزشکی یکبار مصرف : 3. پرتودهی مواد غذایی : 4. کنترل کیفی مواد پلیمری : 5. میکروبیولوژی : 6. انجام پروژه های تحقیقاتی : 7. دزیمتری 119
  • 120. 120 محصولات غذایی مورد تیمار: محصولات خشک شامل حبوبات، ادویه ها و  سبزیجات محصولات غیر غذایی: وسایل بهداشتی، پودر بچه، وسایل استریل جراحی، نخ  های بخیه
  • 121. • خرما: کاهش بار میکروبی و آفات • انار : مقابله با آفت گلوگاه انار • سیب: مقابله با کپک پنی سلیوم • میگو: کنترل ویبریو • گل های زینتی: از بین بردن آفات و شته، رنگ و شمایل متفاوت • تولید آنزیمهای گلوکاناز، پکتیناز از قارچها • افزایش تولید اسید گلوتامیک از کرنی باکتریوم گلوتامیکوم • تولید اسید آمینه لیزین : کاربرد در مواد غذایی و دامی • گیاهان دارویی: تولید ترکیبات معطر جدید • تولید ارقام جدید حاصل از پرتودهی با استفاده از جهش و موتاسیون محصولات کشاورزی یکی از مباحثی بوده که برای اجرای آن بستر لازم توسط محققان مرکز تحقیقات جهاد کشاورزی فراهم شده است. • افزایش توان تولید آستاگزانتین توسط مخمر فافیا که در درمان آب مروارید، آب سیاه و سرطان پستان نقش دارد 121
  • 122. 122 ارزیابی 2 سال پروژه یافتن دز مناسب بهینه سازی بررس ی مشکلات رادیکال های آزاد ارزیابی حس ی
  • 123. • احتمال کاهش رنگ میوه ها و سبزی ها ؛ کاهش رنگ قرمز انار و اثر آنتی اکسیدانی آن • ایجاد موتانتهای جدید باکتری و غیر قابل شناسایی بودن آنها • کامل نبودن زیر ساخت ها، • کاهش چشمه رادیوایزوتوپ ها و طولانی شدن مدت زمان پرتودهی • کمبود منابع مالی و تحریم های پیش رو از جمله چالش های این فناوری )تکمیل مرکز پرتودهی گامای خاورمیانه 110 میلیارد ریال اعتبارنیاز دارد( 123
  • 124. • سامانه پرتودهی چند منظوره گاما در شهرکرد در حال ساخت • ساخت پرتودهی گاما در شیراز • پیشنهاد سازمان انرژی اتمی مبنی بر ساخت سامانه پرتودهی در بناب 124
  • 125. • بیشترین کاربرد در ایران: استرلیزاسیون سرد ادویه ها  دفع آفات حبوبات  • آئین كار پرتودهي ادويه 3102 • تجهیزات پرتودهي مواد غذايي – آئین كار كاربرد 8033 125
  • 126. • ماکیان: 7 کیلوگری • حبوبات: 1 کیلوگری • ادویه ها و چاشنی ها: کنترل آفت: 1 کیلوگری  کاهش بار میکروبی: 10 کیلوگری  • توت فرنگی: 3 کیلوگری 2 کیلوگری / • شیلات: 2 • گندم: 1 کیلوگری • سیب زمینی و پیاز: 1 کیلوگری • خرما: 1 کیلوگری 126
  • 127. Are irradiated foods safe? YES! Radiation doses are never large enough to cause nuclear changes that would cause the food material to become radioactive. 127
  • 128. So, now would you eat an irradiated food product? Why Or Why not? 128
  • 129. 129