2. Learning Objectives
State the importance of correct blood
collection techniques in managing total
patient care.
List the factors that influence the integrity
of a blood specimen.
Discuss safety precautions as related to
blood collection.
Differentiate between whole blood,
plasma, and serum.
3. Learning Objectives
Explain the action of anticoagulants to
prevent blood coagulation.
Describe the appearance of a hemolyzed,
icteric, and lipemic specimen.
Differentiate between arterial, venous, and
capillary blood.
State the purpose of quality assurance in
blood collection.
5. Blood Collection: A Short Course
Purpose
Collection of quality specimens
Instructive information
Technique
Procedures in accordance with the
Clinical and Laboratory Standards
Institute, (CLSI) (Standard H3-A6) and
Occupational Safety & Health
Administration (OSHA) guidelines
6. Blood Collection: A Short Course
Course Overview
Equipment
Site selection
Technique
Complications
Specimen handling
Phlebotomy errors
Venipuncture practice
7. IMPORTANCE OF CORRECT SPECIMEN
COLLECTION AND HANDLING
QUALITY SPECIMENS =
QUALITY RESULTS
Diagnosis
Treatment
Monitoring
Laboratory results constitute 70% of the
objective information for health-care providers
8. IMPORTANCE OF CORRECT SPECIMEN
COLLECTION AND HANDLING
Specimen Integrity
Laboratory guidelines
Never hesitate to check with
laboratory
Clinical Laboratory Improvement
Amendments of 1988 (CLIA, 1988)
Laboratory is responsible
9. IMPORTANCE OF CORRECT SPECIMEN
COLLECTION AND HANDLING
Preanalytical Factors
56% of lab errors occur before the
specimen is tested
Monitoring of specimen ordering
Patient identification
Patient communication and safety
Patient preparation
Timing of collections
Collection technique
Specimen labeling
Specimen handling and transport
15. SAFETY PRECAUTIONS
Accidental Puncture
Significant exposure potential
Report all needlesticks
Follow institution’s protocol for
source and employee testing
16. SAFETY PRECAUTIONS
Syringe-To-Tube Transfer
Recommended procedure
Use blood-transfer device
Removal of stoppers not
recommended
Direct puncture of tube with syringe
needle not recommended
27. TYPES OF SPECIMENS
Venous Blood
Specimen of choice
Used to establish Normal values
Arterial Blood
Blood gases
Require special collection training
28. TYPES OF SPECIMENS
Capillary Blood
Dermal puncture collection
Mixture of arterial and venous blood
Note on requisition if blood is collected by
dermal puncture