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EVE 161:

Microbial Phylogenomics
Class #7:
Era II: rRNA from the Environment
UC Davis, Winter 2018
Instructor: Jonathan Eisen
Teaching Assistant: Cassie Ettinger
!1
Presenters for Today?
Terminology and Questions?
Differences Between the Papers?
Paper #1
!6
Lane et al. Abstract
!7
Lane et al. Background: Why Sequences?
!8
!9
Lane et al. Background: Why Sequences?
Lane et al. Background: Why rRNA
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!11
Lane et al. Background: Why rRNA
Lane et al. Methods: How Get RNA
!12
Methods: How Make Primers
!13
Methods: Reverse Transcriptase
!14
Methods: Nucleotides
!15
Methods: Sequencing
!16
Results and Discussion
!17
Priming
!18
Priming
!19
Priming
!20
Errors
!21
Errors
!22
Alignment
!23
Phylogeny
!24
!26
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Stahl et al. Abstract
!29
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Stahl et al. Introduction
Stahl et al. Introduction
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Stahl et al. Introduction
!33
Stahl et al. Methods
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!35
Stahl et al. Methods
!36
Stahl et al. Methods
`
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Stahl et al. Methods
!38
Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
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Stahl et al. Results
!47
Stahl et al. Results
!48
Stahl et al. Results
!49
Stahl et al. Results
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Stahl et al. Results
!51
Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion
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Stahl et al. Discussion

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EVE161: Microbial Phylogenomics - Class 7 - rRNA from the Environment