Optimized Protocol for Sorting Cells

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This document describes an optimized protocol that enables the co-detection of cluster of differentiation (CD) surface antigens on fixed, permeabilized neural cell populations through combined flow cytometric analysis of both surface and intracellular antigens. This reveals surface molecule markers of human neuropoiesis.

Technologie

Optimized Protocol
enabling the co-detection of cluster of differentiation (CD) surface antigens on fixed, permeabilized neural cell
populations defined by intracellular staining.
Turaç G, Hindley CJ, Thomas R, Davis JA, et al. (2013) Combined Flow Cytometric Analysis of Surface and Intracellular Antigens
Reveals Surface Molecule Markers of Human Neuropoiesis. PLoS ONE 8(6): e68519. doi:10.1371/journal.pone.0068519
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068519

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Optimized Protocol for Sorting Cells

1. Optimized Protocol enabling the co-detection of cluster of differentiation (CD) surface antigens on fixed, permeabilized neural cell populations defined by intracellular staining. Turaç G, Hindley CJ, Thomas R, Davis JA, et al. (2013) Combined Flow Cytometric Analysis of Surface and Intracellular Antigens Reveals Surface Molecule Markers of Human Neuropoiesis. PLoS ONE 8(6): e68519. doi:10.1371/journal.pone.0068519 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068519