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Cytoplasm

Note: The polypeptide
chain then forms into a
three-dimensional figure
(tertiary structure).

AUGAGACCGUAGUGA
Protein Synthesis
By. Anna Heitzenrater
TACTCTGGCATCACT
ATGAGACCGTAGTGA
Cytoplasm

Nucleus
Cytoplasm

Nucleus
Cytoplasm

Nucleus
Nucleus
Cytoplasm

TACTCTGGCATCACT
ATGAGACCGTAGTGA

Nucleus
Cytoplasm

TACTCTGGCATCACT
ATGAGACCGTAGTGA

Nucleus
Cytoplasm

TACTCTGGCATCACT
ATGAGACCGTAGTGA

Nucleus
Cytoplasm

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA
Promoter Region

Nucleus
Cytoplasm

TACTCTGGCATCACT
RNA
Polymerase

mRNA
strand

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

A
U

G

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

AU
G AG

A

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

AU
G AG
AC
CG

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

AU
G AG
AC
C GU
AG

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

AU
G AG
AC
C GU
A G UG A

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA

Nucleus
Cytoplasm

AU
G AG
AC
C GU
A G UG A

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA
Termination
Sequence
Nucleus
Cytoplasm

AU
G AG
AC
C GU
A G UG A

TACTCTGGCATCACT
RNA
Polymerase

ATGAGACCGTAGTGA
Termination
Sequence
Nucleus
A

Cytoplasm

Note: The mRNA must leave
the nucleus through the
nuclear pores to reach the
ribosomes.

C C G
U A GU
G A

GCATCACT

CGTAGTGA
Nuclear Pore

Nucleus
A G
A

Cytoplasm

C C G
U A G U
G

A

GCATCACT

CGTAGTGA

Nucleus
Cytoplasm

G A G
A

C C G
U A G U
G

GCATCACT

CGTAGTGA

Nucleus

A
Cytoplasm

A

U G
AG

GCATCACT

AC

C GU
A GUG A

CGTAGTGA

Nucleus
GCATCACT

Cytoplasm

A

U G
AG
AC
C GU
A GUG A

CGTAGTGA

Nucleus
Cytoplasm

GCATCACT
A

U G
AG

CGTAGTGA

Nucleus

AC

C GU
A GUG A
Cytoplasm

Nucleus

Ribosomes
Cytoplasm

Nucleus

mRNA
strand

Ribosomes
Cytoplasm

Nucleus

Ribosomes
Cytoplasm

Nucleus

Ribosomes
Cytoplasm

Nucleus

Ribosomes
Cytoplasm

Nucleus

Ribosomes
Cytoplasm

Nucleus

Ribosomes
AUGAGACCGUAGUGA
Ribosome
: large
subunit

AUGAGACCGUAGUGA
Small subunit
Cytoplasm
Amino
acid
tRNA

U

A C

Anti-codon

AUGAGACCGUAGUGA
Codon
Cytoplasm

U

A C

AUGAGACCGUAGUGA
Start Codon
Cytoplasm

U
U

C

U

A C

AUGAGACCGUAGUGA
Cytoplasm

U

A C U

C U

AUGAGACCGUAGUGA
Cytoplasm

U

A C
U

C U

AUGAGACCGUAGUGA
Cytoplasm

U

A C
U

C U

AUGAGACCGUAGUGA
Cytoplasm

Peptide Bond

U

C U

AUGAGACCGUAGUGA
Cytoplasm

G

U

G C

C U

AUGAGACCGUAGUGA
Cytoplasm

U

C U G G C

AUGAGACCGUAGUGA
Cytoplasm

U

C U

G G C

AUGAGACCGUAGUGA
Cytoplasm

U

C U

G G C

AUGAGACCGUAGUGA
Cytoplasm

A U C

G G C

AUGAGACCGUAGUGA
Cytoplasm

G G C A U C

AUGAGACCGUAGUGA
Cytoplasm

G G C

A U C

AUGAGACCGUAGUGA
Cytoplasm

G G C

A U C

AUGAGACCGUAGUGA
Cytoplasm

G G C

A U C

AUGAGACCGUAGUGA
Cytoplasm

A U C

A U C

AUGAGACCGUAGUGA
Cytoplasm

Note: The stop
codon does not
make an amino
acid.

A U C A C U

AUGAGACCGUAGUGA

Stop Codon
Cytoplasm

A U C

A C U

AUGAGACCGUAGUGA
Cytoplasm

Polypeptide
Chain

A C U

AUGAGACCGUAGUGA

Stop Codon
Cytoplasm

A C U

AUGAGACCGUAGUGA
Cytoplasm

Note:
The polypeptide chain forms
a three-dimensional figure,
or its tertiary structure.

AUGAGACCGUAGUGA
Summary
First, the enzyme RNA polymerase comes in. It unwinds the double
helix and adds the complementary nucleotides, there is also a
complementary mRNA strand that forms. But, since it is RNA, the
thymine is replaced with uracil. Then, the double helix closes back up
after RNA polymerase goes through. The mRNA exits through the
nuclear pores and travels through the cytoplasm to get to the
ribosomes. Once in the ribosomes, the large and small subunit of the
ribosomes bind with the mRNA. The ribosome begins at the start
codon, that is always AUG. As the ribosome moves down the mRNA
strand, the tRNA molecule, with the correct anti-codon sequence,
then binds onto the codon. As the ribosome keeps on moving, the
amino acids from the previous tRNA falls off and attaches to the next
amino acid. This forms a peptide bond and the tRNA molecule falls
off. This continues until the ribosome reaches the stop codon, the
codon either has a sequence of UAA, UAG, or UGA. The polypeptide
chain, that was formed from the amino acid, then folds into a three
dimensional figure, also known as its tertiary structure.

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