Spermatogenesis in fish involves the process of sperm formation through meiosis and differentiation of spermatogonia into spermatids and sperm. The testis contains seminiferous tubules surrounded by Sertoli cells that support and nourish developing sperm cells. Leydig cells in the interstitial tissue secrete testosterone which regulates meiosis and sperm maturation. Spermatogenesis includes the proliferation, growth, and maturation of spermatogonia through meiosis into spermatids and spermatozoa. The sperm then move through the vas deferens and are released through the urogenital opening.
2. Def :
Proses pembentukan sperma
(spermatogonia)
Meiosis
spermatogenesis
spermiogenesis dan spermiasi
3.
4. TESTIS TERDIRI :
tubulla longitudinalis (cyste seminiferus
mengandung sel-sel spermatogenik yang
berdiferensiasi sinkroni
dikelilingi sel Sertoli / sel Nutrient).
7. SEL SERTOLI (SEL NUTRIENT)
Ciri-ciri :
Bentuk pyramidal panjang bertautan
Melekat lamina basalis
Ujung sel menjorok ke dalam lumen
tubulus / lobulus
Fungsi :
Penyokong, pelindung dan mengatur
nutrisi sel-sel spermatogenik
Fagositosis (produk berlebih)
resorbsi lisosom sel Sertoli
Sekeresi cairan
8. Sel Leydig (sel Interstitial)
Ciri-ciri :
Muncul setelah dewasa
Bentuk bulat / poligonal
Mempunyai inti di tengah
Sitoplasma eosinofil
Fungsi :
Mensekresi hormone testosterone
(meiosis dan maturasi).
Mengatur perkembangan kelamin
sekunder (dimorfisme dan
dikromatisme).
16. SPERMATOGONIA MITOSIS
Spermatosit primer (4n)
spermtosit sekunder (2n)
spermatid (n)
spermatozoa
milt oleh vas deferens
air
17. Spermatogonium Spermatid : Spermatogenesis
Spermatid Spermatozoa : Spermiogenesis
- aparatus golgi Akrosom
- inti kepala spermatozoa
- sentriol ekor spermatozoa
- plasma membran selubung tubuh
- mitokondria berkumpul di ekor
Spermatogonium
- dekat lamina basalis, besar bervariasi
- inti bulat lonjong, + butir-butir kromatin
- nukleoli dekat selaput inti
- sitoplasma granuler, < jelas
- HE = inti bulat, biru (kadang-kadang mitosis)
18. Spermatosit Primer
- Produk akhir spermatogonium B
- inti paling besar, sentral
- interfase = (+) butir-butir kromatin halus
dan rata
Spermatosit Sekunder
- produk meiosis I
- sel tampak bulat ~ spermatogonium
- inti bulat, sentral
- interfase = Inti seperti Spermatosit primer
dengan spermatid muda
19. Spermatid
- produk meiosis II
- hampir berbatasan dengan lumen tubulus seminiferus
- inti eksentris, lonjong
- berkelompok 4 – 8
- daerah golgi dekat inti, berbatas tidak jelas
- mitokondria (butir-butir >>) dalam membran sitoplasma
Spermatozoa
Tubulus seminiferus berkala dewasa kelamin
- mamalia = 50 – 60
- kepala
leher
ekor = - pangkal
- tengah
- ujung
25. FIG. 1. Chinook salmon testes showing stages of germ cell development
Bar =20 µm
primary A spermatogonia transitional spermatogonia late B spermatogonia
primary spermatocytes secondary spermatocytes spermatids (arrow) and spermatozoa (arrowhead)
33. SPERM COMPARISON
NOTE THE 5 MICRON SIZE.
Remember 1000 microns = 1 mm, 25 mm = 1 inch
34.
35. SPERM MOTILITY
The sperm moves by rotating its tail in a spiral motion through
the water. This induces waves of force backward propelling
the sperm forward. If the sperm hits a hard surface, like an
egg, the spiral motion will cause the entire sperm to rotate.
36. SPERM MOTILITY
The sperm moves by rotating its tail in a spiral motion through the
water. This induces waves of force backward propelling the sperm
forward. If the sperm hits a hard surface, like an egg, the spiral
motion will cause the entire sperm to rotate.
37. Sperma bergerak :
Air laut media bersifat hipertonik
Air tawar media bersifat hipotonik
Sperma aktif bergerak dalam air : 30-60’
(hilang setelah 5”).
Sperma ikan teleostei : (-) akrosom
mikrofil (fertilisasi 45 – 60’).