Histology practical #2 demo slides : note cards : some plates
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Dr. Kwiecinski's demo slides with the notecard descriptions in the notes section of the ppt. Just download to view the notes sections. Includes some of the text plates, but not all.
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Histology practical #2 demo slides : note cards : some plates
- 1. Practical #2 Demo Slides
- 2. Week of September 28th Connective Tissue III: Development of Bone
- 11. Week of October 5th Muscle I: Skeletal Muscle
- 19. Muscle II: Smooth & Cardiac Muscle
- 27. Week of 10/12/15 Nervous Tissue I: Neurons; Nerves; Spinal Cord; Cerebellum
- 44. Week of 10/21/15 Nervous Tissue III: Receptors (Sensory Nerve)
- 52. Week of 10/26/15 CVS I: Blood and Blood Vessels
- 71. Lymphatic Tissue I: Bone Marrow Lymphatic Tissue II: Diffuse L.T.; Nodules; Lymph Nodes
- 82. Week of 11/2/15 Lymphatic Tisue III: Tonsils
- 89. Week of 11/9/15 CVS / Lymphatic V: Thymus & Spleen
Notes de l'éditeur
- Foreleg of Fetal Pig PAF , H&E Developing Long Bone Note difference in in hyaline cartilage from previous slide
- Kitten H&E Orange B. Developing Long Bone of Epiphyseal Plate Note: Reaction of hyaline cartilage Epiphyseal and diaphyseal marrow cavities 4 Zones of cartilage in epiphyseal plate and all bone cell types What hormones are responsible for proliferation of hyaline cartilage at epiphyseal plate? What are the hormones responsible for closure of the piphyseal plate
- Endochondral bone formation in leg / Develpoing Mammal Forelimb Can you identify the hyaline cartilage? Can you note any differences in this bone tissue from the previous 3 slides? Can you identify the epiphyseal plate? Note: Epiphyseal Plate Periosteum Perichondrium Primary Center of Ossification Osteoblasts, -cytes, -clasts Bony Spicules Osteocytic Lacunae Chondroind Cores
- Roof of Skull PAF / H&E Note choroid plexus (Significance?) and ependyma lining the ventricles C16 & C17 – Intramembranous Ossification Note: Mesenchymal condensation @ periosteum Osteoblastic differentiation at sites of bone formation
- Osfrontalis Calf Fetus Hem. Eosin Intramembranous bone in the adult is similar in composition and appearance to endochondral bone in the adult Even though the development pattern is different Note: Osteocytic Lacunae Osteoblasts Invading-Differentiating Mesenchyme
- Skeletal Muscle Long Section CAT. PAF Isin(?) - MASSON
- Chick Embryo The myotome of the somite is the origin of myoblasts
- Developing Skeletal Muscle GT Investments – Tendon (inserting into periosteum) are acidophilic while myotubes are basophilic Note: Central nuclei of developing fibers
- C5 & C6 Wrist of Kitten Cross Section and Longitudinal How many different types of CT can you identify? How many types should there be? Can you identify skeletal muscle in cross section and longitudinal section on this slide? FIND: Muscle Fascicles Myotubes Tendons
- 2-3 Day old Rat Embryo Thorax(?) – Developing Trunk Musculature MAY BE MISSING ONE PICTURE Note the skeletal muscle in both longitudinal and cross sections Longitudinal section developing trunk musculature from lateral mesoderm Post myotube stage but not fully differentiated
- Homo Heart Longitudinal PAF – H&E Notes: Intercalated Discs Branching Cells Striations Central Nuclei
- Cardiac Muscle Homo Cross Section H&E Note: Fiber Branching Central Nuclei
- Homo Heart – 82 years Bouin’s H&F PAF – H&E Intercalated Discs Compare to the previous 2 pictures What are the age-related differences found in this slide? Why?
- Ileum (Cat) – Representative of intestinal muscularis externa Peyer’s Patches HEM & EOSIN Note: Circular and longitudinal fibers Which is outer? Went through same staining procedure, but they “look” different. Why? #1 – Zenker’s Fix Acetic Acid, Potassium Dichromate, and Meraric Chloride #2 – PAF Picric Acid, Alcohol, and Formalin = Bouin’s In which layer are corkscrew nuclei easily identified? Identify outer longitudinal and inner circular smooth muscle
- Rat Smooth Muscle Macerated – Teased Contraction Bands What are they? 10& F. tol. Blue Alc. Intestinal smooth muscle cells can be separated by chemicals and mechanical teasing Weak bases and enzymes dissociate (macerate) CT away from cells and mechanical “mixing” Separates groups of cells
- Spinal Cord Felis Faded Nissl Stain However, Nerve Cell Bodies remain obvious Rath’s Nissl Stain w/ Toluidine Blue Right image higher magnification Identify: Ependyma Central Canal Dorsal & Ventral Median Fissures Grey Horns Multipolar Neurons Note: # of Glial Nuclei / Neuron
- Crural Nerve Felis Guess the Stain Zenkers / H&E Identify: Nerve Epi & perineurium Arteries Adipocytes Muscle Type? What are nuclei in nerves? Femoral Vessel & Crural Nerve
- Nerve Trunk – Felis Domesticus Fleming Fix Faded Iron Hematoxylin Can you identify: Nerve Epi/perineurium Artery Vein Adipose Elastin Muscle (Type?) Note: Neurokeratin
- Vagus and Sympathetic Nerve of Cat How was this slide prepared and how dies it differ from the nerve trunk prep? Sudan black – Stains fat/lipids directly Can you identify: Myelin Nerve & NCBs? Adipocytes Epi & perineurium Triads Artery, vein, lymphatic Note: Non-myelinated grouping?
- Duodenum of Sheep Myenteric Plexus Where is it? These nerve cell bodies belong to _______ neurons of the ________ branch of the peripheral nervous system.
- Myenteric Plexus of Auerbach of Chick Where is it? Submucosal Plexus (Present when scanned) These nerve cell bodies belong to _______ neurons of the ________ branch of the peripheral nervous system. What cell bodies (Division of N.S.) are present? How can you tell NCBs from other cells / nuclei
- Sympathetic Ganglion – Dog H&E What criteria allow one to distinguish a dorsal root ganglion from the sympathetic ganglion? What is a name applied to NCBs of sympathetic ganglion (Hint: Anatomical type of Neuron) The nerve cell bodies belong to the ________ neurons of the ________ branch of the peripheral nervous system. What are common glia cells called?
- Stellate Plexus – Kitten Helly fix – Mason Stain (trichrome) What is different between this slide and the previous one? Morphologically? Physiologically? Sympathetic or Parasympathetic? What are your criteria?
- Spinal Ganglion – Cat Fleming’s Fix – Faded Fe2+ Hematoxylin Can you identify: Neurokeratin? Ganglia Cell? Satellite Cell?
- Ileum – Kitten Ganglion cells in the Myenteric and Submucosal Plexi NCBs belong to the ______ neurons of the ______ branch of the peripheral nervous system
- Inner ear of Cat Auditory Organ of Cat Can you find: Spiral Ganglia? Organ of Corti w/ Hair Cells?
- Cochlea – Auditory Organ Hair Cells not sectioned well, but the Organ of Corti is present Note: Spiral Ganglion NCBs
- Sigmund –Is this a Pacinian Corpuscle of human skin? This is really a Meissner’s Corpuscle Can you tell the difference? It’s not Pacinian
- Roof of Skull HOMO PAF H&E Ependyma and Choroid Plexus Significance (what do they do)? Really nervous tissue Glial Note: Ependyma Choroid Plexus
- Sigmund – Olfactory Mucosa Pseudostratified ciliated columnar Thicker (characteristic of olfactory epithelium) than respiratory epithelium Paucity and lack of Goblet Cells Bowman’s Glands Tubuloalveolar Serous Glands w/ Tall Cells Basal Nuclei
- Blood Smear Locate the Lymphocyte in the field of view Locate the small Lymphocyte in the field of view.
- Human Blood Smear Identify the Monocyte and Neutrophils in the field of view
- Human Blood Smear Locate the Basophil in the field of view
- Aorta, Carotid, Femoral Arteries Vena Cava, Jugular, & Femoral Veins Scan slide to view difference b/w arteries and veins
- Composite Blood Vessels – Dog Elastic Trichrome Differences b/w arteries and veins? How does this differ from the previous slide?
- Blood Smear Locate the Eosinophil in the field of view Locate the Monocyte and Small Lymphocyte in the field of view What type of animal is this from? How do you know?
- Hemal Node – Sheep Zenker’s / H&E Not like a Lymph Node In function, more akin to Spleen No nodes, but area of lymphopoiesis Note blood in subcapsular sinus
- Lymph Node – Primate Can you identify: Hilar Region (Approximately)? Subcapsular Sinus? Nodules of Germinated Centers Medullary Cords Mecullary Sinuses
- Lymph Node – Sheep H&E TB + Colch in PAF Note: Trabecular Sinuses (compare to previous slide) Debris in Macrophage / Reticular cells of medullary sinuses
- Liver – Vampire Bat H&E Iron appears as blue/green granules in cytoplasm of R.E.S. neurophages in liver Kupffer Cells – Macrophages or reticular cells; Get what spleen misses Line Blood Vessels
- Bursa Affrabicus – Genesis of B-Lymphocytes Bursa – Young Chick H&E – A.F.A. Zenker Is there an epithelium associated w/ the lymphatic tissue? If so, characterize it. Is there another name which can be applied to the bursa? Young Chick
- Colon – Monkey Isolated Lymph Nodule H&E Lymph nodules in colon are located in the ________, a C.T. layer of the G.I. tract.
- Bone Marrow
- Bone Marrow Smear Identify: Various Erythropoietic Cells Various Granulopoietic Cells
- Appendix - Homo
- Human Palantine Tonsil Tonsil – Palantine H&E Characterize this epithelium Where do you think this tonsil came from? Note: Size of “immune” cells to epithelial cells Germinal Center
- Pheryngeal Tonsil – Adenoid – Homo H&E Characterize this epithelium. Where do you think this tonsil came from? Note: Germinal Center
- Bursa Affrabicus – Genesis of B-Lymphocytes Bursa – Young Chick H&E – A.F.A. Zenker Is there an epithelium associated w/ the lymphatic tissue? If so, characterize it. Is there another name which can be applied to the bursa? Young Chick
- Spleen – Kitten Helly’s H&E Compare to slide 92 Can you identify ellipsoids as easily?
- Uvula Any glands? Lymphatic Tissue? Type of muscle and CT?
- Spleen – Kitten Helly’s Masson Ellipsoids should be easy to find Compare to slide 90
- Spleen – Vampire Bat H&E Megakaryocytes or foreign body giant cells? Nore: Trabeculae – Smooth Muscle
- Thymus – Kitten Very very lightly stained but Thymic Corpuscles are distinguishable Hematoxylin gone
- Spleen – Skunk HgI2 and H&E
- Spleen – Dog PAF Masson
- Thymus Is this old or young?