1. Environmental Factors in Women’s
Reproductive Health: Impact on
Child Health and Development
Leslie Rubin MD
Morehouse School of Medicine
Developmental Pediatric Specialists
Innovative Solutions for Disadvantage and Disability
Southeast Pediatric Environmental Health Specialty Unit at Emory University
2. Human health problems on the rise
• Over the past 30 years, there
has been a significant rise in
many childhood disorders as
well as in hormonal and
reproductive disturbances
• Early life exposure to
environmental pollutants is a
leading suspect.
• Some chemicals are directly
toxic to an exposed child while
other chemicals induce a chain
of events that may culminate
in a diagnosed health problem
later in life.
3. Children are More Vulnerable to
Environmental Factors
• A developing child's chemical exposures are greater pound-for-
pound than those of adults
• Children have lower levels of some chemical-binding
proteins, allowing more chemical to reach target organs
• Systems that detoxify and excrete industrial chemicals are
not fully developed
• An immature, porous blood-brain barrier allows greater
chemical exposures to the developing brain
• Organs and systems are rapidly developing, and thus are
often more vulnerable to damage from chemical exposure
National Academy of Sciences 1993
4. Pediatric
Environmental Health
• The child’s environment
as a sum of
– Chemical
– Physical
– Biological &
– Social Factors
• The environment can impact the
child’s growth, development, and
well-being for better or worse
5. Boston
New England PEHSU
New York City
Mount Sinai PEHSU
Washington DC
Mid-Atlantic Center for Children’s
Health & the Environment PEHSU
Atlanta
Southeast PEHSU
Canada
Kansas City
Mid-America PEHSU
Tyler
Southwest Center PEHSU
Seattle
Northwest PEHSU
San Francisco
University of California PEHSU
Denver
Rocky Mountain PEHSU
Chicago
Great Lakes Centers PEHSU
Irvine
University of California PEHSU
Guadalajara, Mexico
PEHSU
Mexico
Alaska
Hawaii
Virgin
Islands
Puerto
Rico
Edmonton, Canada
PEHSU
6. Vulnerability of the Fetus
• However vulnerable a child is to
environmental factors, the embryo and
fetus are much more sensitive to
disruptions even at low doses
7.
8. Critical Periods in Development
Central nervous system (3 wks - 20 yrs)
Ear (4-20 wks)
Kidneys (4-40 wks)
Heart (3-8 wks)
Immune system (8-40 wks; competence & memory birth-10 yrs)
Limbs
(4-8
wks)
Skeleton (1-12 wks)
Lungs (3-40 wks; alveoli birth-10 yrs)
Reproductive system (7-40 wks; maturation in puberty)
Source: Altshuler, K; Berg, M et al. Children's Health and the Environment, February 2003.
9. Thalidomide
• Thalidomide first entered the German market in
1957 as a sedative, tranquilizer, and antiemetic.
• It was also proclaimed a "wonder drug" for
insomnia, coughs, colds, and headaches.
• It was advertised as “completely safe” for
everyone, including mother and child, “even
during pregnancy,” as its developers “could not
find a dose high enough to kill a rat.”
• By 1960, thalidomide was marketed in 46
countries, with sales nearly matching those of
aspirin!
10. Thalidomide
• In 1961, reports began
to emerge associating
the drug with severe
birth defects
• By March of 1962, the
drug was banned in
most countries where it
was previously sold
• An estimated 10-20,000
infants were born with
this condition
11. Thalidomide - Lessons
• The field of Teratology
was given a start and
research was directed to
finding what other drugs
caused birth defects
• Regulations became
more stringent on testing
drugs for safety during
pregnancy
12. Minamata Disease
• On April 21, 1956, a five
year-old girl was examined
at the Chisso Corporation's
factory hospital in
Minamata, Japan,
• The physicians were puzzled
by her symptoms: difficulty
walking, difficulty speaking
and convulsions.
• They soon found other
family members and many
other children with the
condition
13. Minamata Disease
• On May 1, the hospital director reported an
"epidemic of an unknown disease of the
central nervous system“ - marking the official
discovery of Minamata disease
• By the end of the year Minamata disease was
considered to be a result poisoning by a
methyl mercury that entered the body
through consumption of fish and shellfish
from the Minamata Bay
14. Minamata Disease
• Chisso Corporation, a
chemical company
located in Kumamoto
Japan, had dumped an
estimated 27 tons of
mercury compounds
into Minamata Bay.
• As of March 2001,
2,265 victims had been
officially recognized
15. Lessons Learned
• The placenta does not
provide full protection
• Mercury can cause brain
damage in the unborn
fetus
• Other heavy metals and
toxins can do the same
• Toxic chemicals need to
be regulated
• We need to monitor the
consumption of fish
during pregnancy
16. FDA Guidelines for
Children and Pregnant Women
• No more than 12-oz of
“low” mercury fish, (e.g.
Cod & Canned Tuna) should
be consumed weekly.
• “High" mercury fish, (e.g.
Sea Bass & Bluefish) should
be kept to only three 6-oz
servings per month.
• "Highest" mercury fish, (e.g.
Marlin & Swordfish) should
be completely avoided
17. Congenital Rubella
• Between 1963 and 1965 a rubella
epidemic swept the nation….
• Children were mildly affected,
but…
• It caused 30,000 miscarriages and
• 20,000 pregnant women who
contracted the disease gave birth
to infants with congenital
anomalies and
neurodevelopmental disorders
18. Lessons Learned
• Infections in pregnant
women (even supposedly
mild ones) can have major
consequences on their
unborn fetus
• Other similar conditions
were recognized - ToRCH
• Immunizations against
childhood illnesses not only
prevents complications in
the children but prevents
potential effects on the
fetus during pregnancy
19. Fetal Alcohol Syndrome
• In 1973 Jones and Smith published a report on
‘the first reported association between maternal
alcoholism and aberrant morphogenesis in the
offspring’ – the main features are
– pre and/or postnatal growth retardation,
– characteristic facial abnormalities, and
– central nervous system dysfunction, including mental
retardation
• Studies by the CDC report prevalence rates of FAS
alone from 0.2 to 1.5 cases per 1,000 births
across various populations
Smith, et al (1973) Lancet i, 1267–1271.
20. FAS – Lessons Learned
• Alcohol and other drugs
taken by the mother
during pregnancy can
have a deleterious
effect on the growth
and development of the
fetus and child
• Warning labels on
bottles of alcohol and
education of public
21. Smoking During Pregnancy
• The embryos of
mothers who smoked
took 62 hours to reach
the eight-cell stage,
compared to 58 hours
in non-smokers
• Increasing risk of low
birth weight,
prematurity and other
consequences on
embryogenesis
Freour et al University of Nantes Presented at ESHRE meeting in Turkey 2012
22. Long-Term Consequences of
Fetal and Neonatal Nicotine Exposure
• Studies suggest that nicotine may be a key
chemical responsible for many long-term effects
associated with maternal cigarette smoking on
the offspring, such as
– hypertension
– type 2 diabetes, obesity
– respiratory dysfunction
– neurobehavioral defects
– impaired fertility
Bruin et al Toxicological Sciences 116: 364-374 2010
23. Tobacco – Lessons Learned
• The effects of the
toxin not only have a
direct effect on the
smoker and on the
growth of the fetus
• but can have long
term health
implications for the
rest of that child’s life
• And for that child’s
offspring as well
24. Diethylstilbestrol (DES):
The Promise
• Diethylstilbestrol (DES) is a synthetic estrogen
that was developed to supplement a woman's
natural estrogen production.
• First prescribed in 1938 for women who
experienced miscarriages or premature deliveries
• DES was originally considered effective and safe
for both the pregnant woman and the fetus
25. Diethylstilbestrol (DES):
Alarms!
• In 1971, the FDA issued a Drug Bulletin advising
physicians to stop prescribing DES to pregnant
women because it was linked to a rare vaginal
cancer in female offspring.
• An estimated 5-10 million people in the USA
were exposed to DES during 1938-1971
26. Diethylstilbestrol (DES):
What we Know Now
• Women prescribed DES while pregnant are at a
modestly increased risk for breast cancer.
• Women exposed to DES in utero, are at an
increased risk for:
– clear cell adenocarcinoma (CCA) of the vagina and
cervix,
– reproductive tract structural differences
– pregnancy complications, and
– infertility.
• Men exposed to DES in utero are at an increased
risk for non-cancerous epididymal cysts.
27. Lessons Learned
• We cannot assume any
drug or chemical is
completely safe even if
we do not see immediate
consequences
• The effect may only
manifest later in life or in
the next generation
• Therefore we need to
adopt the precautionary
principle
28. Chemicals in our Environment
• EPA lists >80,000 chemicals in the USA
• 3,000-4,000 are ‘high volume’ >1million
pounds per year
• 700 new chemicals introduced into the USA
per year
• The Toxic Substances Control Act (TSCA)
authorizes EPA to test <200 chemicals
Sutton et al AJOG 2012
30. • Researchers at two major laboratories found an
average of 200 industrial chemicals and pollutants in
umbilical cord blood from 10 babies born in August and
September of 2004 in U.S. hospitals.
• Tests revealed a total of 287 chemicals in the group.
• The umbilical cord blood of these 10 children, collected
by Red Cross after the cord was cut, harbored
– pesticides,
– consumer product ingredients, and
– wastes from burning coal, gasoline, and garbage.
Environmental Working Group, July 14, 2005
34. Neurodevelopmental Scores and
Prenatal Exposure to Chlorpyrifos
• 265 children in a prospective study
– measured prenatal Chlorpyrofos exposure using umbilical
cord blood plasma and
– 7-year neurodevelopment using the Wechsler Intelligence
Scale for Children, 4th edition (WISC-IV).
• Results: On average, for each standard deviation
increase in Chlorpyrofos exposure (4.61 pg/g),
– Full-Scale intelligence quotient (IQ) declined by 1.4% and
– Working Memory declined by 2.8%!
Rauh et al. 2011 Environ Health Perspect
35. Agricultural Pesticides
and Autism
• Children of mothers living within 500 m of
field sites with the highest amount of
organochlorine pesticides had a 6x greater
chance of having a child with Autism
compared to those with mothers not living
near the field sites
Roberts et al Environ Health Perspect. 2007 Oct;115(10):1482-9.
36. Endocrine disruptors
• Chemicals that may interfere with the body’s endocrine system
• Endocrine Disruptor Chemicals:
– pharmaceuticals, dioxins, polychlorinated biphenyls (PCB’s), pesticides, and
plasticizers such as bisphenol A (BPA)
• Endocrine disruptors may be found in
– plastic bottles, metal food cans, flame retardants, detergents, food, toys
and cosmetics
• Endocrine disruptors pose the greatest risk during prenatal and
early postnatal development when organ systems are forming
37. Consequences of
Endocrine Disruptors
• For women:
– Breast and reproductive organ tissue cancers, fibrocystic disease
of the breast, polycystic ovarian syndrome, endometriosis,
uterine fibroids and pelvic inflammatory diseases.
• For men:
– Poor semen quality (low sperm counts, low ejaculate volume,
high number of abnormal sperm, low number of motile sperm),
testicular cancer, malformed reproductive tissue (undescended
testes, small penis size), prostate disease
• In children:
– congenital anomalies of genitalia (e.g. hypospadius),
neurodevelopmental disorders, immune disorders, thyroid
disorders, precocious puberty.
38. Should We Be Concerned?
Sharpe and Irvine, 2004 - Slide Curtesy of Linda Birnbaum NIEHS 2012
39. Decreasing Age of Puberty
US expert panel concluded:
• Earlier breast development and
onset of menarche
• “Suggest … endocrine-disrupting
chemicals …and
body fat are important factors
associated” with the change
• African American and Mexican
American girls enter puberty
earlier than white girls
Age of Menarche in Europe and the US
from 1790 to 1980
Euling et al. Pediatrics 2008 - Slide Curtesy of Linda Birnbaum NIEHS 2012
.
40. Environmental Living Conditions
of Poor Communities
• In old housing with poor indoor air quality, mold and
deteriorating lead based paint
• Unsafe Neighborhoods due to: Crime, Traffic Density,
Litter & Trash, Poor Lighting Adjacent to major roadways
• More likely to be exposed to a variety of toxins from a
variety of sources
• Lack of or limited green space and parks
• Poor quality schools
• Limited access to quality health care
41.
42. 42
Cumulative risk
Poverty
Lead &
other toxins
Allergens
Poor nutrition
Stressed
mother
Noise
Unsafe
neighborhood
No parks
From Lecture by Dr. Howard Frumkin
44. Epigenetics
• Environmental Factors do not change the DNA but change
the expression of genes by the process of methylation
Adapted from Linda Birnbaum NIEHS 2012
45. Epigenetic Changes
and Human Diseases
EPIGENOME DISEASE
Adverse health outcomes
Cancer
Cardiopulmonary disease
Autoimmune disease
Obesity
Diabetes
Neurodevelopmental disorders
Schizophrenia
Addiction
Depression
Normal processes
Development
Cell differentiation Aging
GENOME
External influences
Environmental exposures
Nutrition
Chemical toxins
Metals
Mediators of stress
Drugs of abuse
Infection (including HIV)
Slide Cutesy of Linda Birnbaum NIEHS 2012
46. Conclusions
• The fetus is exquisitely sensitive to environmental
influences
• These influences may cause immediate or long
term consequences on the health, growth and
development of the child and even on the child’s
reproductive health and into the next generation
• It is our responsibility to assure that pregnant
women are not unnecessarily exposed to
environmental factors that could be harmful to
them or their fetus
We know that there are different stages of development, from the first week of the embryo to the postnatal years, even through puberty.
And the standard biomedical paradigm has been that exposures during development will affect the organ system in question.
The key point to recognize is that there are “windows of susceptibility” throughout all stages of development.
The US Geological Survey (USGS) monitors ground and surface water for 76 pesticides and seven pesticide breakdown products. A recent survey found that 90% of streams and 50% of wells tested were positive for at least one pesticide.
Ref:
USGS: ca.water.usgs.gov/pnsp/index.html
A great potential for adverse effects of pesticides is through contamination of the hydrological system, which supports human life, aquatic life and related food-chains.
Ref:
USGS: ca.water.usgs.gov/pnsp/atmos/atmos_1.html
Over the past fifty years, we’ve seen increases in health problems such as breast and prostate cancer, ectopic pregnancies, undescended testicles, and a 42% decrease in sperm count.
These findings, along with observations of abnormal sexual development in frogs and fish, and the widespread detection of endocrine disrupting chemicals in our bodies, led NIEHS to increase its research on the effects of these chemicals on human health.
A U.S. consensus panel, that included scientists from EPA and NIEHS, published its findings in 2008. There was general agreement, given the science that’s already out there, that the age of puberty has decreased, specifically breast development and onset of menarche.
There was also agreement that the studies suggest that endocrine-disrupting chemicals and body fact are associated with this altered timing of puberty.
Epigenetics:
Our understanding of chemical toxicity has been challenged by the new science of epigenetics, which is the study of changes to the packaging of the DNA molecules that influence the expression of genes, and hence the risks of diseases and altered development. Studies indicate that exposures that cause epigenetic changes can affect several generations. This new understanding heightens the need to protect people at critical times in their development when they are most vulnerable to this kind of toxicity.
Epigenetics is the study of changes in DNA expression that are independent of the DNA sequence itself. Although a person’s DNA base sequence doesn’t change, the expression of that DNA into a person’s phenotype can be altered by methylation of cytosine residues in DNA, modification of the histones which package the DNA into chromatin, and control of chromatin structure via microRNAs.
This has significant implications for the cause and heritability of disease.
I think it’s safe to say that the first 10 years of this century were all about the gene. I believe that the next 10 years will be all about the epigenome.
Clearly, epigenetics is creating a new paradigm in our understanding of disease. Whereas in the past we believed that there was a simple relationship between environmental exposures and disease – that is, you get exposed, and the exposure causes disease – we now understand that the effects are more complex.
When we say “the Environment” most people might just think of harmful chemicals, but it really involves a large array of exposures, including nutrition, metals, drugs, infectious diseases, and even stress.
And complex diseases have complex etiologies. Cancer, cardiopulmonary disease, autoimmune diease, obesity, diabetes, nerurodevelopmental disorders, schizophrenia, addition and depression are just some of the diseases where we know that the environment plays a significant role in their development.
So our thinking about environmental exposures and its effects have changed significantly. And there is a huge impact to this new understanding – if we develop a better understanding of the epigenetic mechanisms of disease, we have the opportunity for prevention, and targeted therapeutics.
So, given this paradigm change, we have new goals in the field of epigenetics. Our research should attempt to determine epigenetic targets in the genome that are sensitive to modification by environmental exposures. We can examine the changes in epigenetic markers over time and correlate these changes with environmental exposures.