LC MS/MS....

1. LIQUID CHROMATOGRAPHY
MASS SPECTROMETER MASS
SPECTROMETER
LC- MS/ MS

3. INTRODUCTION
 liquid chromatography + mass spectrometry
 physical separation and mass analysis
 Identify variety of compounds
 high sensitivity and specifity

4. LIQUID CHROMATOGRAPHY
 HPLC
 Principle:
 Pump pass pressurized liquid solvent
 Sample pass through a column
 Each component interacts differently with adsorbent
 Separation of the components

6. TANDEM MASS SPECTROMETRY
 MS/MS
 MS1:
 precursor ions are formed
 separate by m/z ratio
 Daughter ions are created by fragmentation
 MS2:
 Ions are separated and detected

7. TANDEM MASS SPECTROMETERY

8. COMPONENTS
ION
SOURCE ANALYZER FRAGMENTATION DETECTOR

9. ION SOURCE
Electron Spray Ionization (ESI)
Atmospheric Pressure Photo
Ionization (APPI)
Atmospheric Pressure Chemical
Ionization(APCI)

10. ELECTRON SPRAY IONIZATION

11. ATMOSPHERIC PRESSURE PHOTO IONIZATION

12. ATMOSPHERIC PRESSURE CHEMICAL IONIZATION

13. Mass Analyzer
Time Of
Flight
Quadrupole Ion Trap

14. TIME OF FLIGHT

15. QUADRUPOLE

16. ION TRAP

17. FRAGMENTATION

18. DETECTOR

20. ADVANTAGES
SPECIFITYSELECTIVITY
TIME
MULTIPLE
COMPOUND
ANALYSIS
ABUNDANCE OF
INFORMATION
COST

21. APPLICATIONS
Endocrinology
New born
screening
Therapeutic
drug
monitoring
Steroid testing
Pain
management
testing
Drugs of
abuse
Clinical
analysis

22. WHAT IS THE
DIFFERENCE
BETWEEN LC-MS and
LC-MS/MS ???