Column chromatography

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COLUMN CHROMATOGRAPHY Column of stationary phase is used » Solid – S.P » Liquid – M.P PRINCIPLE ◊ Adsorption Mixture of components dissolved in the M.P is introduced in to the column. Components moves depending upon their relative affinities.

PRACTICAL REQUIREMENTS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

SELECTION OF S.P ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

◊ MOBILE PHASE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

PREPARATION OF THE COLUMN ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Dry Packing Technique ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

INTRODUCTION OF THE SAMPLE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Gradient elution techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

RECOVERY OF COMPONENTS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Advantages of C.C ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Column chromatography

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Column chromatography