Ce diaporama a bien été signalé.
Nous utilisons votre profil LinkedIn et vos données d’activité pour vous proposer des publicités personnalisées et pertinentes. Vous pouvez changer vos préférences de publicités à tout moment.
Histologic evidence ofHistologic evidence of
SPIO accumulation inSPIO accumulation in
vulnerable plaquesvulnerable plaques...
In-vitro Study of Macrophage
SPIO Uptake
 In a series of in-vitro studies we have tested
the rate of SPIO uptake by human...
FL-labeled SPIO Incubated Macrophages after 24hr
Double DAPI Staining with Fluorescence-labeled SPIO Macrophages
after 24hr Incubation
Macrophages labeled with
fluorescent microspheres adhere
to atherosclerotic plaques.
In-vivo distribution of SPIO in ApoE
deficient and wild type mice:
•For the initial study, we use the mouse model of
ather...
•Tissues from different organs including liver,
kidneys, lung, heart, spleen and aorta
(including valve region, ascending,...
ApoE k/o Mouse 5 Days AfterApoE k/o Mouse 5 Days After
Injection- LiverInjection- Liver
Histopathologic Study of the Mice Injected
with SPIO (Aortic Root)
ApoE K/O mouse, Movat staining, aortic root
Coronary
Cr...
Histopathologic study of ApoE K/O Mouse injected
With SPIO (Aortic Root)
CD68 staining
(coronary plaque)
Iron Staining (ao...
Histopathologic Study of Wild Type Mouse
Injected with SPIO (Thoracic Aorta)
H&E staining
CD68 stainingIron staining
Comparison of the Number of the Iron Particles (per
HPF) in ApoE K/O Mice Plaque vs. Normal Wall
0
5
10
15
Atherosclerotic...
Histopathologic studies of Thoracic aorta in Watanabe
Hereditary Hypercholesterolemic rabbit after SPIO injection
H&E stai...
Hypercholesterolemic Rabbit, Aorta, 4 days after
SPIO injection
Perls’ Staining H&E Staining
X40 X40
Electron Microscopy Evidence of Intracellular
SPIO in the Rabbit Aorta
Endothelial cell, x7500 Foamy cell, x4000
0
10
20
30
40
50
60
0 10 20 30 40 50 60 70
macrophage (foam cell) density
SPIOpositivecell-Iron
staining
Series1
R=0.956
C...
Plaque Iron in ApoE k/o mousePlaque Iron in ApoE k/o mouse
after SPIOafter SPIO
H&E IRON STAIN
Plaque Iron in Cytokine-TreatedPlaque Iron in Cytokine-Treated
Mice after SPIOMice after SPIO
H&E stain Iron stain
Plaque Iron in Cytokine–Treated MicePlaque Iron in Cytokine–Treated Mice
after SPIOafter SPIO
Overlying plaques Plaque-fre...
Conclusion:
1) SPIO nanoparticles actively accumulate in
superficial
areas of atherosclerotic lesions in rabbits and mice....
Center for Vulnerable Plaque ResearchCenter for Vulnerable Plaque Research
Houston, TexasHouston, Texas
Prochain SlideShare
Chargement dans…5
×

053 histologic evidence of spio accumulation in vulnerable plaques

177 vues

Publié le

SHAPE Society

Publié dans : Santé & Médecine
  • Identifiez-vous pour voir les commentaires

  • Soyez le premier à aimer ceci

053 histologic evidence of spio accumulation in vulnerable plaques

  1. 1. Histologic evidence ofHistologic evidence of SPIO accumulation inSPIO accumulation in vulnerable plaquesvulnerable plaques Silvio Litovsky, MDSilvio Litovsky, MD June 7, 2002June 7, 2002
  2. 2. In-vitro Study of Macrophage SPIO Uptake  In a series of in-vitro studies we have tested the rate of SPIO uptake by human activated monocytes in different conditions regarding incubation time and concentration of SPIO. SPIO particles were labeled by a fluorescent dye (DCFA).
  3. 3. FL-labeled SPIO Incubated Macrophages after 24hr
  4. 4. Double DAPI Staining with Fluorescence-labeled SPIO Macrophages after 24hr Incubation
  5. 5. Macrophages labeled with fluorescent microspheres adhere to atherosclerotic plaques.
  6. 6. In-vivo distribution of SPIO in ApoE deficient and wild type mice: •For the initial study, we use the mouse model of atherosclerosis. •ApoE deficient mouse has similar atherosclerotic lesions to human and the lesions are more common in the aortic arch and thoracic aorta. • We used ApoE deficient mice and normal variant (C57BL mice) as control. •Animals were sacrificed on day 3 and 5 after injection.
  7. 7. •Tissues from different organs including liver, kidneys, lung, heart, spleen and aorta (including valve region, ascending, descending and abdominal) were obtained and stained. • We used Hematoxyline and Eosin (H&E), Iron, CD68 and Movat staining. • After Iron staining, aortic walls of the ApoE deficient mice and normal variant were compared based on the number of Iron particles.
  8. 8. ApoE k/o Mouse 5 Days AfterApoE k/o Mouse 5 Days After Injection- LiverInjection- Liver
  9. 9. Histopathologic Study of the Mice Injected with SPIO (Aortic Root) ApoE K/O mouse, Movat staining, aortic root Coronary Cross section Atherosclerotic plaque
  10. 10. Histopathologic study of ApoE K/O Mouse injected With SPIO (Aortic Root) CD68 staining (coronary plaque) Iron Staining (aortic plaque) Iron Staining (coronary section) Iron particles Iron particles
  11. 11. Histopathologic Study of Wild Type Mouse Injected with SPIO (Thoracic Aorta) H&E staining CD68 stainingIron staining
  12. 12. Comparison of the Number of the Iron Particles (per HPF) in ApoE K/O Mice Plaque vs. Normal Wall 0 5 10 15 Atherosclerotic Aorta Average number of iron particles per sample P <0.001
  13. 13. Histopathologic studies of Thoracic aorta in Watanabe Hereditary Hypercholesterolemic rabbit after SPIO injection H&E staining Macrophage staining Iron staining Iron particles
  14. 14. Hypercholesterolemic Rabbit, Aorta, 4 days after SPIO injection Perls’ Staining H&E Staining X40 X40
  15. 15. Electron Microscopy Evidence of Intracellular SPIO in the Rabbit Aorta Endothelial cell, x7500 Foamy cell, x4000
  16. 16. 0 10 20 30 40 50 60 0 10 20 30 40 50 60 70 macrophage (foam cell) density SPIOpositivecell-Iron staining Series1 R=0.956 Correlation Between Iron Positive Cells in Iron Staining and Foam Cell Density in H&E Staining in Rabbit Atherosclerotic Aorta.
  17. 17. Plaque Iron in ApoE k/o mousePlaque Iron in ApoE k/o mouse after SPIOafter SPIO H&E IRON STAIN
  18. 18. Plaque Iron in Cytokine-TreatedPlaque Iron in Cytokine-Treated Mice after SPIOMice after SPIO H&E stain Iron stain
  19. 19. Plaque Iron in Cytokine–Treated MicePlaque Iron in Cytokine–Treated Mice after SPIOafter SPIO Overlying plaques Plaque-free area
  20. 20. Conclusion: 1) SPIO nanoparticles actively accumulate in superficial areas of atherosclerotic lesions in rabbits and mice. 2) There is a strong correlation between areas of foamy cells infiltration and SPIO uptake in mice and rabbit plaques. 3) Cytokines markedly increase the SPIO uptake by atherosclerotic plaques, thus providing a method for quantifying recruitment of monocytes. 4) MRI has the potential for non-invasively assessing monocyte homing into atherosclerotic plaque.
  21. 21. Center for Vulnerable Plaque ResearchCenter for Vulnerable Plaque Research Houston, TexasHouston, Texas

×