SHAPE Society

1. SPIO and T2 Effect
In vitro study to show the effect
of macrophage SPIO uptake on
their T2 relaxation time

2. Protocol:
 We used 8 flasks of CBM macrophages.
 After preparing the cells, Feridex was
added with the proper concentration to
each labeled tube.
 Incubation was done at 37 C.
 For each time, pellet the tubes at 1000
rpm’s for 5 min.
 Washed with 1X PBS for 5 min 3 times.
 Resuspended in 2% paraformaldehyde, to
fix the cells.

3. time
concentration
20
Min
1
Hour
6
Hours
24
Hours
50µl 100µl 250µl 500µl control control
Expected T2 Reduction Effect

6. Macrophage Uptake of Feridex After 20
Min Shown by T2 Reduction
0
10
20
30
40
50
60
70
80
90
50 100 250 500 control control
20 min
Concentration µl

7. 0
10
20
30
40
50
60
70
80
90
50 100 250 500 control control
60 min
Macrophage Uptake of Feridex After 60
Min Shown by T2 Reduction
Concentration µl

8. 0
10
20
30
40
50
60
70
80
90
50 100 250 500 control control
6 Hours
Macrophage Uptake of Feridex After 6hr
Shown by T2 Reduction
Concentration µl

9. 0
10
20
30
40
50
60
70
80
90
50 100 250 500 control control
24 Hours
Macrophage Uptake of Feridex After 24hr
Shown by T2 Reduction
Concentration µl

10. 0
10
20
30
40
50
60
70
80
90
50 100 250 500 control control
20 min
60 min
6 hours
24 hours
Macrophage Uptake of Feridex with Time
and Concentration Shown by T2
Reduction
Concentration µl

11. 0
10
20
30
40
50
60
70
80
90
20 Min 60 Min 6 Hours 24 Hours
50
100
250
500
control
control
Macrophage Uptake of Feridex with
Concentration and Time Shown by T2
Reduction
µl

12. •SQUIDS are extremely sensitive detectors for magnetic fields
Summary:
•They utilize 3 quantum mechanical effects: Zero resistance, Flux quantization, and
Josephson effect.
What can we do with it?
SQUIDs can be used to as a noninvasive screening test for detecting super paramagnetic or
paramagnetic particles in site of inflammation, infection or injury in body
Examples:
Myocardial infarction
atherosclerotic plaque (vulnerable plaque)

13. Myocardial infarction
Acute MI
Q-wave
Non-Q wave
Short axis cine Short-axis contrast
50 y/o male admitted with chest pain and
precordial ST segment elevation.
Patient was treated with primary angioplasty
but Q-waves eventually developed in leads
1,L, avL.
• Peak CK: 5912; MB: 792
• Cath Report: 100% occluded left circumflex
coronary artery which was opened with PTCA
and stented.
• MRI: performed 2 weeks after acute MI.
The lateral wall is akinetic (cine MRI) and has
transmural hyperenhancement (ceMRI).

14. Myocardial infarction
Acute MI
Q-wave
Non-Q wave
Case history:
• 52 y/o male admitted with minor chest pain and
nonspecific ECG findings.
Echocardiogram was normal but the patient eventually
ruled in for a non Q-wave MI.
• Peak CK: 560; MB: 62
• Cath Report: 100% occluded 1st Diagonal off the LAD.
No other significant CAD was found.
• MRI: performed 6 days after acute MI. Wall motion is
normal (cine MRI) but a small non-transmural region
of hyperenhancement is seen in the anterolateral wall
(ceMRI).
short-axis cine short-axis contrast

15. Insert Gd vs SPIO
charts here