Primer Designing

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mohammad shenagari

Primer Design

1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)

Gene Runner 1.Sequence Insertion

Oligo 6

Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)

HCV Genome

Alignment

Current oligo length Position number

Non specific band

- Length ,[object Object],[object Object]

Contenu connexe

Tendances

RNA-Seq

Bioinformatics and Computational Biosciences Branch

2d Page

microbiology Notes

Pyrosequencing

DNA SEQUENCING METHOD

DNA SEQUENCING METHOD

DNA SEQUENCING METHOD

Next generation sequencing

Next generation sequencing

Next generation sequencing

Pyrosequencing

Comparative genomics

Comparative genomics

Comparative genomics

Q pcr

Labelling of dna

Labelling of dna

Labelling of dna

Molecular probes kashmeera n.a.

Molecular probes kashmeera n.a.

Molecular probes kashmeera n.a.

Chromosome walking

Chromosome walking

Chromosome walking

Sivasangari Shanmugam

Maxam–Gilbert sequencing

Maxam–Gilbert sequencing

Maxam–Gilbert sequencing

Obydulla (Al Mamun)

Dna sequencing

Ravi Kant Agrawal

Dna sequencing

Sanger sequencing

Sanger sequencing

Sanger sequencing

Raghavendra Kulkarni

Dna sequencing methods

Dna sequencing methods

Dna sequencing methods

NGS: Mapping and de novo assembly

NGS: Mapping and de novo assembly

NGS: Mapping and de novo assembly

Bioinformatics and Computational Biosciences Branch

Introduction to next generation sequencing

Introduction to next generation sequencing

Introduction to next generation sequencing

VHIR Vall d’Hebron Institut de Recerca

Next generation sequencing

Next generation sequencing

Next generation sequencing

Swathi Prabakar

cohesive and blunt end ligation

cohesive and blunt end ligation

cohesive and blunt end ligation

Tendances (20)

RNA-Seq

2d Page

Pyrosequencing

DNA SEQUENCING METHOD

DNA SEQUENCING METHOD

DNA SEQUENCING METHOD

Next generation sequencing

Next generation sequencing

Next generation sequencing

Pyrosequencing

Comparative genomics

Comparative genomics

Comparative genomics

Q pcr

Labelling of dna

Labelling of dna

Labelling of dna

Molecular probes kashmeera n.a.

Molecular probes kashmeera n.a.

Molecular probes kashmeera n.a.

Chromosome walking

Chromosome walking

Chromosome walking

Maxam–Gilbert sequencing

Maxam–Gilbert sequencing

Maxam–Gilbert sequencing

Dna sequencing

Dna sequencing

Sanger sequencing

Sanger sequencing

Sanger sequencing

Dna sequencing methods

Dna sequencing methods

Dna sequencing methods

NGS: Mapping and de novo assembly

NGS: Mapping and de novo assembly

NGS: Mapping and de novo assembly

Introduction to next generation sequencing

Introduction to next generation sequencing

Introduction to next generation sequencing

Next generation sequencing

Next generation sequencing

Next generation sequencing

cohesive and blunt end ligation

cohesive and blunt end ligation

cohesive and blunt end ligation

Dernier

The presentation explores the development and application of artificial intelligence (AI) from its inception to its current status in the modern world. The term "artificial intelligence" was first coined by John McCarthy in 1956 to describe efforts to develop computer programs capable of performing tasks that typically require human intelligence. This concept was first introduced at a conference held at Dartmouth College, where programs demonstrated capabilities such as playing chess, proving theorems, and interpreting texts. In the early stages, Alan Turing contributed to the field by defining intelligence as the ability of a being to respond to certain questions intelligently, proposing what is now known as the Turing Test to evaluate the presence of intelligent behavior in machines. As the decades progressed, AI evolved significantly. The 1980s focused on machine learning, teaching computers to learn from data, leading to the development of models that could improve their performance based on their experiences. The 1990s and 2000s saw further advances in algorithms and computational power, which allowed for more sophisticated data analysis techniques, including data mining. By the 2010s, the proliferation of big data and the refinement of deep learning techniques enabled AI to become mainstream. Notable milestones included the success of Google's AlphaGo and advancements in autonomous vehicles by companies like Tesla and Waymo. A major theme of the presentation is the application of generative AI, which has been used for tasks such as natural language text generation, translation, and question answering. Generative AI uses large datasets to train models that can then produce new, coherent pieces of text or other media. The presentation also discusses the ethical implications and the need for regulation in AI, highlighting issues such as privacy, bias, and the potential for misuse. These concerns have prompted calls for comprehensive regulations to ensure the safe and equitable use of AI technologies. Artificial intelligence has also played a significant role in healthcare, particularly highlighted during the COVID-19 pandemic, where it was used in drug discovery, vaccine development, and analyzing the spread of the virus. The capabilities of AI in healthcare are vast, ranging from medical diagnostics to personalized medicine, demonstrating the technology's potential to revolutionize fields beyond just technical or consumer applications. In conclusion, AI continues to be a rapidly evolving field with significant implications for various aspects of society. The development from theoretical concepts to real-world applications illustrates both the potential benefits and the challenges that come with integrating advanced technologies into everyday life. The ongoing discussion about AI ethics and regulation underscores the importance of managing these technologies responsibly to maximize their their benefits while minimizing potential harms.

Artificial Intelligence: Facts and Myths

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As privacy and data protection regulations evolve rapidly, organizations operating in multiple jurisdictions face mounting challenges to ensure compliance and safeguard customer data. With state-specific privacy laws coming up in multiple states this year, it is essential to understand what their unique data protection regulations will require clearly. How will data privacy evolve in the US in 2024? How to stay compliant? Our panellists will guide you through the intricacies of these states' specific data privacy laws, clarifying complex legal frameworks and compliance requirements. This webinar will review: - The essential aspects of each state's privacy landscape and the latest updates - Common compliance challenges faced by organizations operating in multiple states and best practices to achieve regulatory adherence - Valuable insights into potential changes to existing regulations and prepare your organization for the evolving landscape

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MySQL Webinar, presented on the 25th of April, 2024. Summary: MySQL solutions enable the deployment of diverse Database Architectures tailored to specific needs, including High Availability, Disaster Recovery, and Read Scale-Out. With MySQL Shell's AdminAPI, administrators can seamlessly set up, manage, and monitor these solutions, ensuring efficiency and ease of use in their administration. MySQL Router, on the other hand, provides transparent routing from the application traffic to the backend servers in the architectures, requiring minimal configuration. Completely built in-house and supported by Oracle, these solutions have been adopted by enterprises of all sizes for their business-critical applications. In this presentation, we'll delve into various database architecture solutions to help you choose the right one based on your business requirements. Focusing on technical details and the latest features to maximize the potential of these solutions.

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With more memory available, system performance of three Dell devices increased, which can translate to a better user experience Conclusion When your system has plenty of RAM to meet your needs, you can efficiently access the applications and data you need to finish projects and to-do lists without sacrificing time and focus. Our test results show that with more memory available, three Dell PCs delivered better performance and took less time to complete the Procyon Office Productivity benchmark. These advantages translate to users being able to complete workflows more quickly and multitask more easily. Whether you need the mobility of the Latitude 5440, the creative capabilities of the Precision 3470, or the high performance of the OptiPlex Tower Plus 7010, configuring your system with more RAM can help keep processes running smoothly, enabling you to do more without compromising performance.

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Scaling API-first – The story of a global engineering organization Ian Reasor, Senior Computer Scientist - Adobe Radu Cotescu, Senior Computer Scientist - Adobe Apidays New York 2024: The API Economy in the AI Era (April 30 & May 1, 2024) ------ Check out our conferences at https://www.apidays.global/ Do you want to sponsor or talk at one of our conferences? https://apidays.typeform.com/to/ILJeAaV8 Learn more on APIscene, the global media made by the community for the community: https://www.apiscene.io Explore the API ecosystem with the API Landscape: https://apilandscape.apiscene.io/

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Abhishek Deb(1), Mr Abdul Kalam(2) M. Des (UX) , School of Design, DIT University , Dehradun. This paper explores the future potential of AI-enabled smartphone processors, aiming to investigate the advancements, capabilities, and implications of integrating artificial intelligence (AI) into smartphone technology. The research study goals consist of evaluating the development of AI in mobile phone processors, analyzing the existing state as well as abilities of AI-enabled cpus determining future patterns as well as chances together with reviewing obstacles as well as factors to consider for more growth.

Exploring the Future Potential of AI-Enabled Smartphone Processors

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Increase engagement and revenue with Muvi Live Paywall! In this presentation, we will explore the five key benefits of using Muvi Live Paywall to monetize your live streams. You'll learn how Muvi Live Paywall can help you: Monetize your live content easily: Set up pay-per-view access to your live streams and start generating revenue from your content. Increase audience engagement: Provide exclusive, premium content behind the paywall to keep your viewers engaged. Gain valuable viewer insights: Track viewer data and analytics to better understand your audience and tailor your content accordingly. Reduce content piracy: Muvi Live Paywall's security features help protect your content from unauthorized distribution. Streamline your workflow: The all-in-one platform simplifies the process of managing and monetizing your live streams. With Muvi Live Paywall, you can take control of your live stream monetization and create a sustainable business model for your content. Learn more about Muvi Live Paywall and start generating revenue from your live streams today!

Top 5 Benefits OF Using Muvi Live Paywall For Live Streams

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This presentation explores the impact of HTML injection attacks on web applications, detailing how attackers exploit vulnerabilities to inject malicious code into web pages. Learn about the potential consequences of such attacks and discover effective mitigation strategies to protect your web applications from HTML injection vulnerabilities. for more information visit https://bostoninstituteofanalytics.org/category/cyber-security-ethical-hacking/

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Boston Institute of Analytics

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Three things you will take away from the session: • How to run an effective tenant-to-tenant migration • Best practices for before, during, and after migration • Tips for using migration as a springboard to prepare for Copilot in Microsoft 365 Main ideas: Migration Overview: The presentation covers the current reality of cross-tenant migrations, the triggers, phases, best practices, and benefits of a successful tenant migration Considerations: When considering a migration, it is important to consider the migration scope, performance, customization, flexibility, user-friendly interface, automation, monitoring, support, training, scalability, data integrity, data security, cost, and licensing structure Next Wave: The next wave of change includes the launch of Copilot, which requires businesses to be prepared for upcoming changes related to Copilot and the cloud, and to consolidate data and tighten governance ShareGate: ShareGate can help with pre-migration analysis, configurable migration tool, and automated, end-user driven collaborative governance

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+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...

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Bajaj Allianz Life Insurance Company - Insurer Innovation Award 2024

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Primer Designing

1. Primer Design

2. 1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)

3. Gene Runner 1.Sequence Insertion

4.

5.

6.

7.

8.

9.

11.

12.

13.

14.

15.

16.

17. Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)

20.

21. Current oligo length Position number

22. Non specific band

23.

24.

25.

26.

27.

28.

29.

30.

31.

32.

33.

34.