how to check the adulterant in the milk

2.  Milk is the best cheapest source of the nutrition, easily
accepted and used by the all age groups in the rural
area
 It provide the acceptable amount of the protein ,fat
vitamin.
 Milk is the largest and the single commodity within
the live stock.
 It contains 1% fat as well as energy 42 calories.
 term "milk" comes from "Old English meoluc (West
Saxon)
What is the milk

3. Top ten cow milk producers
in 2013 Rank -:
Country Production
 (metric tonnes)
 1 United States 91,271,058
 2 India 60,600,000
 3 China 35,310,000
 4 Brazil 34,255,236
 5 Germany 31,122,000
 6 Russia 30,285,969
 7 France 23,714,357
 8 New Zealand 18,883,000
 9 Turkey 16,655,009
 10 United Kingdom 13,941,000

5. Adulteration
 It is an act of intentionally debasing the quality of the
milk either by the mixture or substitution of the
inferior substances.
 It is the removal of the valuable substances from the
milk that are injurious to the health.
 ‘Adulterant’ means any material that make the food
unsafe or substandard or misbranded.
 Any material is added in the milk which reduces the
nutritional value of the milk as well as injurious to the
health is called milk adulteration.

6. Some adulterant in the milk
generally use-:
 Water
 Starch
 Urea
 Detergent
 Synthetic milk
 Glucose/invert sugar
 formalin
 Ammonium sulphate

9. Materials
 Milk
 Test tubes
 Water both
 Incubator
 Conc HCL
 Conc H2SO4
 Ferric chloride
 2%NaOH
 5%phenol

11.  Detection of the microorganism
 Detection of the table sugar
 Detection of the starch
 Detection of the acids
 Detection of the soap
 Detection of the formalin
 Detection of the Ammonium sulphate
Procedure-

12. Detection of the microorganism
 Measure out 10 ml milk In a test tube
 Measure 1 ml methylene blue indicator.
 Mixe in the test tube well.
 Kept in the incubator at 37Oc for 30 min.
 After incubation colour changes blue to white means
micro organism present.
 If colour does not change means microorganism is not
present.

14. Detection of the starch
 Measure 3ml milk in the test tube.
 The test tube is then kept for the incubation in boiling
water both for 10 min.
 After incubation ,test tube is cooled at the room
temperature.
 Adding the iodine solution and mix the content well.
 Appearance the black colour presence starch in the
milk.

16. Detection of the acid
 Measure the 5 ml milk in the test tube.
 Add few drops conc.H2SO4,shaking well.
 Adding 0.5%ferric chloride solution drop wise in to the
test tube.
 Mix the content well.
 Development of buff colour is indicative of the
presence of benzoic acid.

18. Detection of the soap
 Measure 10 ml milk in to test tube.
 Add 10 ml of hot water in to the test tube containing
milk.
 Now add 1-2 drops phenolphthalein indicator.
 Gently mixed the content of the tubes.
 Appearance of the pink colour on addition of
phenolphthalein ,soap present.

21. Detection of the formalin
 Measure the 2ml milk in the test tube.
 Adding the 2 ml of 90% sulphuric acid and ferric
chloride.
 Both content are mixed well.
 Formation of the purple colour ring at the interface of
the two layer means formalin present in the milk.

24. Detection of the ammonium
sulphate
 Measure the 2ml milk in the test tube.
 Adding 1-5 drop of NaOHin the test tube as well as
adding the 2-8dro p phenol in to the test tube.
 Mix the content thoroughly.
 A light bluish colour will be observed.
 Keep the test tube in the water both for 20 second.
 Presence of the ammonium sulphate is indicated by
the formation of the deep blue colour in the milk after
taking the water both.