1. T T T
1681.8397 1681.8397 1681.8397
5 5 5
T T7 T7 T7
T T
T 1481.8099 1995.1389 T 1481.8099 1995.1389 1481.8099 1995.1389
T T T T 37 T T T 37
909.4676 2495.27912837.3934 37
3519.8990
12 1492.7842 909.4676 3519.8990 909.4676 3519.8990
12 12
2495.27912837.3934 2495.27912837.3934
2298.37632704.3640 61.
3501.0 1492.7842 61.
3501.0 1492.7842 61.
3501.0
16 5
2298.37632704.3640 2298.37632704.3640
1 16 1 16 1
325 14 5 325 14 5 325 14
1000 1500 2000 2500 3000 3500
24 1000 1500 2000 2500 3000 350024 1000 1500 2000 2500 3000 350024
ISB
DIBIP
www.isbiotechnology.com
Via Fantoli 16/15 (Milano)
Director : Dr. Simone Cristoni
Mail to : info@isbiotechnology.com
DynamIc BIomarker Profile
Early stage cancer diagnosis is possible now
Ion Source & Biotechnologies
1

2. T T T
1681.8397 1681.8397 1681.8397
5 5 5
T T7 T7 T7
T T
T 1481.8099 1995.1389 T 1481.8099 1995.1389 1481.8099 1995.1389
T T T T 37 T T T 37
909.4676 2495.27912837.3934 37
3519.8990
12 1492.7842 909.4676 3519.8990 909.4676 3519.8990
12 12
2495.27912837.3934 2495.27912837.3934
2298.37632704.3640 61.
3501.0 1492.7842 61.
3501.0 1492.7842 61.
3501.0
16 5
2298.37632704.3640 2298.37632704.3640
1 16 1 16 1
325 14 5 325 14 5 325 14
1000 1500 2000 2500 3000 3500
24 1000 1500 2000 2500 3000 350024 1000 1500 2000 2500 3000 350024
ISB
www.isbiotechnology.com
Via Fantoli 16/15 (Milano)
Director : Dr. Simone Cristoni
Mail to : info@isbiotechnology.com
Open source
metabolic profiling
Ion Source & Biotechnologies
Early stage cancer diagnosis through
2

3. Apparatus for early stage cancer Diagnsosis
DynamIc Biomarker Profile (DIBIP)
+ +
OnLine
DIBIP
LC-MS USIS Data
elaboration
system
References
1) Cristoni S. et al. Rapid Commun Mass Spectrom. 2009 Sep;23(17):2839-45
2) Cristoni S. PCT International patent n. (WO 2007/131682)
3

4. DIBIP data collection and elaboration description
 Creation of different mass spectra library (ex. 2 libraries: controls and patients).
 The mass spectra are inserted in the correct library to create an effective database (to
be enlarged by further insertion of other spectra).
Each mass spectrum represents the entire plasma peptide & metabolite profile of a
person. (*, or an other biological fluid)
 An unknown sample is directly compared with each single profile of the database and it is
automatically allocated in one or in the other library by similarity.
CONTROLS’ LIBRARY
PATIENTS’ LIBRARY
UNKNOWN SAMPLE
The whole plasma metabolite profile of an unknown sample is directly
compared with each MS spectrum of both libraries and classified by similarity.
4

5. DIBIP diagnosis data elaboration flow
Plasma from Plasma from Plasma from
unknown subjects control subjects unhealthy patients
DIBIP data DIBIP data DIBIP data
Control library
Control library Unhealthy library
DIAGNOSIS
5

6. Results
94 % of Correct diagnosis!!!!!!!
Sensitivity: 94%
Selectivity: 94%

7. Results
Elaboration platform will be made freely
available at:
http://www.isbiotechnology.com/

8. T T T
1681.8397 1681.8397 1681.8397
5 5 5
T T7 T7 T7
T T
T 1481.8099 1995.1389 T T 1481.8099 1995.1389 T 1481.8099 1995.1389
T 37 T T 37 T T 37
909.4676 12
2495.27912837.3934 3519.8990
909.4676 3519.8990 909.4676 3519.8990
1492.7842 12 12
2495.27912837.3934 2495.27912837.3934
2298.37632704.3640 61.
3501.0 1492.7842 61.
3501.0 1492.7842 61.
3501.0
16 5
2298.37632704.3640 2298.37632704.3640
1 16 1 16 1
325 14 5 325 14 5 325 14
1000 1500 2000 2500 3000 3500 1000
24 1500 2000 2500 3000 350024 1000 1500 2000 2500 3000 350024
Team
Dr. Cristina Canton Biologist
Dr. Martina Soldaini Marketing
Dr. Ombretta Repetto Biologist
ISB
Dr. Alessandro Finiguerra Chemist
Dr. Simone Cristoni General Manager
Dr. Lorenzo Zingaro Laboratory Manager
Dr. Vincenza Flora Dibari Bioinformatician
Dr. Pablo Martínez-Lozano Marie Curie researcher
Ion Source & Biotechnologies
8