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PPARg KD i 3T3-L1 celler
Problem ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Setup
Setup ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 4/3    1/1,5   1/5 Crude transduktionseffektivitet (PPARg dag 2) Oprenset transduktionseffektivitet (PPARg dag 2) Transduktionseffektivitet ,[object Object]
Crude transduktionseffektivitet (Lacz dag 6)  4/3    1/1,5   1/5 ,[object Object],Oprenset transduktionseffektivitet (pSico dag 6) Transduktionseffektivitet
Transduktionseffektivitet  4/3    1/1,5   1/5 ,[object Object],Crude transduktionseffektivitet (pSico dag 4) Oprenset transduktionseffektivitet (pSico dag 4)
Transduktionseffektivitet Oprenset transduktionseffektivitet (pSico dag 6)  4/3    1/1,5   1/5 ,[object Object],Crude transduktionseffektivitet (pSico dag 6)
Transduktionseffektivitet Crude transduktionseffektivitet (pSico dag 4)  4/3    1/1,5   1/5 ,[object Object],Oprenset transduktionseffektivitet (pSico dag 6)
Differentieringsgrad  4/3    1/1,5   1/5 ,[object Object],Differentieringsgrad for oprenset LacZ (dag 6) Differentieringsgrad for crude LacZ (dag 6)
[object Object],[object Object],[object Object]
Tom virus ,[object Object],Differentieringsgrad for crude pSico (dag 6) ,[object Object],Differentieringsgrad for crude LacZ (dag 6)
Tom virus  4/3    1/1,5   1/5 Differentieringsgrad for oprenset pSico (dag 6)  4/3    1/1,5   1/5 Kontrol: oprenset LacZ (dag 6)
[object Object],[object Object]
Puromycin-kassetten  4/3    1/1,5   1/5 ,[object Object],Differentieringsgrad for oprenset Scramble (dag 6) Differentieringsgrad for crude Sramble (dag 6)
CEBP KD ,[object Object],Differentieringsgrad for crude CEBPb (dag 6) ,[object Object],Differentieringsgrad for crude LacZ (dag 6)
CEBP KD  4/3    1/1,5   1/5 Differentieringsgrad for oprenset CEBPb (dag 6)  4/3    1/1,5   1/5 Kontrol: oprenset LacZ (dag 6)
[object Object],[object Object]
CEBPb’s differentiering
PPARg Knock Down Crude virus (dag 2) Oprenset virus (dag 2) 1:5   1:1,5  4:3  1:5   1:10
C/EBPb Knock Down Crude virus (dag 2) Oprenset virus (dag 2) 1:5   1:1,5  4:3  1:5   1:10

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PPARg KD i 3T3-L1 celler

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  • 13. Tom virus 4/3 1/1,5 1/5 Differentieringsgrad for oprenset pSico (dag 6) 4/3 1/1,5 1/5 Kontrol: oprenset LacZ (dag 6)
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  • 17. CEBP KD 4/3 1/1,5 1/5 Differentieringsgrad for oprenset CEBPb (dag 6) 4/3 1/1,5 1/5 Kontrol: oprenset LacZ (dag 6)
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  • 20. PPARg Knock Down Crude virus (dag 2) Oprenset virus (dag 2) 1:5 1:1,5 4:3 1:5 1:10
  • 21. C/EBPb Knock Down Crude virus (dag 2) Oprenset virus (dag 2) 1:5 1:1,5 4:3 1:5 1:10