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Jon Fong
Dept. of Vertebrate Zoology &
Anthropology
Ichthyology Section
Radiography in Research
X-rays and Ichthyology
Why radiography?
2
Why radiography?
3
“Turning to living fishes, the higher classification of these is based to a
large extent… on comparative osteology.”
“When fishes are skeletonized, the soft parts are lost along with many
of the smaller bones, and unless great care is taken the relative
positions of many of the parts no longer can be determined.”
Why radiography?
4
“Another method of osteological work is that of clearing and staining.”
“The difficulties here are that the process is tedious, the soft parts
again are lost for all practical purposes, and the specimens must be
kept thereafter in glycerin. Because of the various limitations of
this technique, clearing and staining can advantageously be applied
only to quite small specimens.”
Why radiography?
5
“… a [radiograph] makes available for fish classification a meristic
character not otherwise easily accessible, namely the number of
vertebrae.”
“…there is one category of material on which no method other than X-
rays can be employed for osteological work. This is the type, or very rare,
specimen. On such material the X-ray alone does not injure or alter the
specimen.”
Primary Types Imagebase
6
•Over 1600
primary type lots
•NSF-funded,
1999-2001
•Photographs,
radiographs, and
illustrations
http://research.calacademy.org/research/ichthyology/types/index.asp
A chance to upgrade equipment
7
GE grain inspector
Picker dental x-ray machine
developing trays
protective screen
New equipment
8
Faxitron model 43855C
Konica SRX-101A
Film cassette
Mixed results
9
ca. 2 cm
Equipment Plan B
10
•Manual processing tank
•Industrex film
Insert picture of industrex film redipak
Better results
11
Pros
•Finer grain = more detail
•Better contrast
•More latitude
•Can shoot bigger specimens
Cons
•Much longer processing time
•Messier
Digitization
12
Microtek 9600XL flatbed scanner with transparency
adapter
•1200 x 600 dpi
•Scannable area
11 x 15 in.
•File sizes 100KB-
180MB
Project results
13
Exposed film storage
14
Plates stored in filing cabinets
Incidental benefits
15
Digital photography
16
Nikon Coolpix 990
Sony Mavica MVC-FD91
Tank shooting
17
Light stand shooting
18
Improvements in photography
19
Sony Mavica MVC-FD91
Big Kahuna
Future considerations
• Digital sensor
• Microfocus x-ray to enable enlarged images
• Phase-contrast radiography
20
This afternoon…
21

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