Virtual Proteomics Analysis Cluster in the Cloud

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This talk was presented at Super Computing '09 in the Cloud Computing for Systems and Computational Biology workshop. It describes the proteomics analysis package we built using Amazon's cloud computing architecture. More information in our paper in J. Proteome Research http://pubs.acs.org/doi/abs/10.1021/pr800970z

Santé & Médecine Technologie

Its always sunny on top of the Cloud! An intro to Amazon Web Services ,[object Object],[object Object],ViPDAC, a stand-alone Proteomics Analysis Suite in the Cloud

‘ How the humble pipette tip helped us rethink our computing strategy...’

Proteomics - Finding and identifying proteins DB Rat/Tissue Sample LC MS/MS Peptide Identification Results & Analysis

Current architecture Windows (head node, preprocessing, storage) Raw File .dtas Protein IDs IBM Blade Cluster (Sequest)

Finite Resource, wait your turn 1 MCW Cluster

Here’s the lab’s pipette tip, Let me have it when you’re done...

What would you do if there was only one tip? ,[object Object],[object Object],[object Object],[object Object],[object Object]

OK, more computers might be better... but... we dont have the money! we dont have an IT guy/gal we dont have a sysadmin we dont know how to install a cluster we wont use it all the time

Virtual Proteomics Analysis Cluster (ViPDAC) http://proteomics.mcw.edu/vipdac + +

Current architecture with Sequest Raw File .dtas Protein IDs IBM Blade Cluster (Sequest) Windows (head node, preprocessing, storage)

ViPDAC & Amazon Components S3 (Data Store) Raw File .dtas Protein IDs EC2 (OMSSA, !XTandem)

ViPDAC & Amazon Components S3 (Data Store) Raw File .dtas Protein IDs 2x 3x 20x

Wait in line vs On Demand vs 1 MCW Cluster Molly’s ViPDAC Shama’s ViPDAC Brian’s ViPDAC Bassam’s ViPDAC

Equal-opportunity computing - Clusters for All vs 1 PC 1 ViPDAC or n ViPDACs

Observations Sign up & Start up is hard for biologists. http://www.directthought.com / http://www.elasticpod.com /

Now what? ,[object Object],[object Object],[object Object],[object Object],[object Object],Molly’s ViPDAC Shama’s ViPDAC Brian’s ViPDAC Bassam’s ViPDAC

Internal Hybrid Solution – Local and Cloud Scale up/down/off

Clouds & Bioinformatics: Our observations so far ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Acknowledgements ,[object Object],[object Object],[object Object],[object Object],Low Cost, Scalable Proteomics Data Analysis Using Amazon’s Cloud Computing Services and Open Source Search Algorithms. J. Proteome Res., 2009, 8 (6), pp 3148–3153

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