SMGT is a simple and efficient technique to produce transgenic mice is a transgenic technique, uses the sperms as a natural vector to transport the internalize exogenous DNA into an oocyte during fertilization to produce genetically modified organisms.

1. Sperm Mediated Transgenesis
C.Sworna kumari

2. SPERM MEDIATED GENE TRANSFER
(SMGT)
•SMGT is a simple and efficient technique to
produce transgenic mice
• is a transgenic technique, uses the sperms
as a natural vector to transport the internalize
exogenous DNA into an oocyte during
fertilization to produce genetically modified
organisms.
•Use of linker protein to attach DNA to sperm
which transfer the DNA during fertilization

3. Sperm-Mediated Gene Transfer Mechanism

4. MOLECULAR MECHANISM OF
SPERM MEDIATED GENE TRANSFER
•natural vector, sperm cell
•The exogenous DNA molecules bind to the sperm cells
head in the subacrosomal region and in the proximity of
the equatorial area.
•DNA interacts with DNA-Binding proteins ON the sperm
cell surface,
• as well as major histocompatibility complex MHC- class
II and CD4 proteins also involved in the interaction
process,
•Once bound to the cell membrane, DNA molecules are
taken up.

5. Different methods for Improving the Sperm cell
transfer

6. Electroporation based SMGT

7. Linker based SMGT (LB-SMGT)

8. Retroviral based SMGT

9. Liposome based (lipofection) SMGT

10. Restriction enzyme mediated
integration SMGT (REMI-SMGT)

12. References
• R. Kumar Pramod & Abhijit Mitral, “Intratesticular injection
followed by electroporation allows gene transfer in caprine
spermatogenic cells” SCIentIfIC REPOrTS | (2018) 8:3169 |
DOI:10.1038/s41598-018-21558-9
• Mohammed Al-Shuhaib et al .,”Sperm Mediated Gene
Transfer in Mammals; a Versatile Platform with Multiple
Enhancements Techniques”. ISSN 2224-3208 (Paper) ISSN
2225-093X (Online) Vol.4, No.5, 2014 .
• Marialusia lavtrana et al.,”Sperm mediated gene transfer”.
Reproduction, fertility and development,2006, 18,19-23.
• Kevin Smith and Corrado Spadafora, “Sperm-mediated gene
transfer: applications and implications” BioEssays 27:551–
562, 2005 Wiley Periodicals, Inc.

13. THANK YOU