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Biotechnology Chapter 7.
Bio pesticides
Pesticide
 A pesticide is any substance or mixture of substances intended for
preventing , destroying , repelling , or mitigating any pest.
 Pest include insects, plant pathogens, weeds, birds, and microbes
etc.
Classification on the basis of Targeted
Organism
Pesticides
Algicides
Virucides
Rodenticide
Fungicides
Herbicides
insecticide
Avicides Bactericide
miticides
nematicide
Types Of Pesticides
Chemical Pesticides
 1. Organochlorides, which have a high persistence in the
environment of up to about 15 years (DDT, dieldrin and aldrin
 2. Organophosphates, which have an intermediate persistence of
several months (parathion, carbaryl and malathion)
 3. Carbamates, which have a low persistence of around two weeks
(Tenik, Zectran and Zineb)
 4. Synthetic pyrethroids, which are nonpersistent, contact and
residual acting insecticides (cypermethrin, permethrin), suitable for
a wide range of crops and target insect
Biopesticides
 Biopesticides are certain type of pesticides derived from such
natural materials as plants, animal and microbes like bacteria,
fungi etc.
Microbial Pesticides
 Microbial pesticides are composed of microscopic living organisms (viruses,
bacteria, fungi, protozoa, or nematodes) or toxin produced by these organisms
 Applied as conventional insecticidal sprays, dusts, or granules.
 Their greatest strength is their specificity as most are essentially nontoxic and
non pathogenic to animals and humans.
 Microbial pesticides includes insecticides, fungicides, herbicides and growth
regulators of microbial origin.
Some of the important microbial pesticides
a. Bacillus thuringiensis
 Discovered in Japan in early 20th century and first become a
commercial product in France in 1938.
 Control lepidopterous pests like
American bollworm in cotton
and stem borers in rice.
Fig: Bacillus thuringiensis
 When ingested by pest larvae, Bt releases toxins which damage the mid
gut of the pest, eventually killing it.
 Main sources for the production of Bt preparations are the strains of the
subspecies kurstaki, galeriae and dendrolimus
e. Metarizium anisopliae
•It infects spittlegbugs, rhinoceros beetles.
f. Beauveria bassiana
• Controls Colorado potato beetle.
g. Verticillum lecanii:
•Controls aphids and whiteflies.
h. Nomuraea riley:
• Controls soybean caterpillars.
i.Baculoviruses (Bvs)
• Control lepidopterous and hymenopterous pests.
•Rod shaped, circular double stranded super coiled DNA.
Fig: Metarhizium anisopliae
Fig: Beauveria
 Pesticides derived from plants
 Generally act in one of two ways
 Contact poison
 Stomach poison
 About 250000 plant species evaluated
 2121 useful in pest management
 1005 exhibited insecticidal activity
 384antifeedants
 297 repellents
 27 attractants
 31 growth inhibiting properties
Plant-incorporated- protectants (PIPs)
 Pesticidal substances that plant produce from the genetic material that
has been added to the plant.
 As the pest feed on such plants they will eventually die.
Botanical pesticides:
 These are naturally occurring plant material that may be crude
preparation of the plant parts ground to produce a dust or powder that
can be used in full strength or dilute form in a carrier such as clay, talc
or diatomaceous earth.
 “Azadirachtin” effects the reproductive and digestive process of pest.
 Several plant based insecticides as nicotinoids, natural pyrethroids,
rotenoids, neem products etc are used.
4.Biochemical pesticides
 They are naturally occurring substance to control pest by non-toxic
mechanisms.
 Biochemical pesticides include substances as insect sex pheromones, that
interfere with mating that attract insect pest
to traps.
 The synthetic attractants-
are used in one of four ways:
i. As a lure in traps used to monitor pest populations;
ii. As a lure in traps designed to “trap out” a pest population;
iii. As a broadcast signal intended to disrupt insect mating
iv. As an attractant in a bait containing an insecticide
Fig: weevil pheromone trap
 Biopesticides are usually inherently less toxic than conventional
pesticides.
 Biopesticides generally affect only the target pest and closely
related organisms,
 Biopesticides often decompose quickly, avoiding the pollution
problems caused by conventional pesticides.
 Biopesticides can greatly decrease the use of conventional
pesticides, while crop yields remain high.
 Cheap, renewable can be handled safely.
 Difficult for insects to develop resistance to these pesticides.
 Slow effect
 Lack persistence and wide spectrum activity
 Rapidly degraded by UV lights so residual action is slow.
 Seasonal availability of plants products indicates the needs for storage.
 They are not available easily
 Poor water solubility and generally not systemic in nature
 All products applied followed by growers have not been scientifically
verified.
Fate of Pesticides
Once a pesticide is applied, several things may happen.
 It may be taken up by plants and/or ingested by animals, insects,
worms, or microorganisms in the soil
 It may move downward in the soil and adhere to soil particles, or it
may dissolve
 It may volatilize
 It may be broken down into less toxic compounds
 It may be leached or moved out of the plants root zone by rain or
irrigation water or
 It may be carried away by runoff water or erosion
Factors Affecting Fate Of Pesticides
There are four major factors which affect the fate of pesticides.
They are as follows:
1. Properties of the pesticide
2. Properties of the soil
3. Conditions of the site
4. Management practices
Biosafety Levels
Biosafety Levels
• Biosafety: Containment principles, techniques and practices implemented
to avoid unintentional exposure to pathogens or toxins or their accidental
release.
• Biosecurity : Protection, control and accountability for valuable biological
materials in laboratories, in order to restrict access and avoid loss, theft,
misuse, diversion or deliberate release
 Biological materials:
 Samples (blood, urine, tissue, secretions etc.) in common use in
laboratories and research units are a source of risk sometimes
identified but often unrecognized and difficult to assess
 Microorganisms including those which are genetically modified, cell
cultures and human endoblasts capable of causing infection, allergy
or intoxication
 Biological risk: Probability of being exposed to a biological hazard
- Directly: agent triggering the disease
- Indirectly: toxin
 Biological agents are divided into 4 hazard groups:
 By the pathogenicity of biological agents
 By the risk of illness of the exposed person
 By the risk of spread in the community
 By the existence of a prophylaxis or an effective treatment
Groups Characteristics Examples
1 Does not cause disease in humans. Lactobacillus, Adenovirus,
E. coli…
2 Could cause illness in humans and be a danger to workers ;
Their spread in the community is unlikely ; There is usually
effective prophylaxis or treatment.
Staphylococcus aureus,
influenza virus, measles
virus, Hepatitis virus A, B,
C, E, Plasmodium…
3 Could cause serious illness in humans and pose a serious
danger to workers ; Spread to the community is
possible, but there is usually effective prophylaxis or
treatment
SARS-CoV-2, Yellow fever
virus, Dengue virus,
HIV, Rift valley fever
virus, West Nile virus…
4 Cause serious illness in humans and pose a serious danger
to workers ; The risk of their spread to the community is
high ; There is usually no prophylaxis or effective treatment.
Lassa virus, Ebola virus,
Crime Congo virus …
Classification of biological agents
Facilities: Types of laboratory – Biosecurity level and corresponding
Microbiological Safety Cabinets
Containment
level
Local Specific
equipment
Good practices
BSL1
Room ventilated and insulated by a
door and closed windows. Easily
washable benches, walls and floors.
Autoclave in the
building
Protective clothing. Clean and
tidy mats. GLP
BSL2
BSL1 + Regulated access for
authorized personnel , making of
premises, hermetic closure for
fumigation (optional), sinks with non-
manual controls. Autoclave
Microbiological
safety cabinet
(MSC)
Secure
centrifuges.
PPE : Blouse, gloves, glasses,
use of needle box, use of
disposable materials,
inactivation of contaminated
material (alcohol 70 etc.) and
waste
BSL3
BSL2 + airlock, filtration of incoming
and outgoing air, intercom (optional),
negative pressure with alarm system,
generator, shower (optional)
MSC II, double
entry autoclave
Same layouts as in BSL2
+ wearing overboots and
overcoats
BSL4
BSL3 + Emergency ventilation
system, intercom compulsory, double
airlock, shower compulsory
MSC III
Same layouts as in BSL3
+ use a protective suit
MSC I
MSC II
MSC III
Workspace organization under MSC
Clean area Work zone Contaminated area
Facilities:
 Appropriate size, structure and location
 Space to avoid contamination of samples with separation of
activities, limited access
 Storage areas: controlled temperature, defined limits, alarm, backup
 Good maintenance
Waste disposal pathways
Waste disposal pathways
• minimise waste and do not accumulate large amounts in the laboratory. Regular disposal
from the laboratories must be part of the laboratory WHS program.
• segregate waste - have a separate residue container if you are generating a large
amount of any particular type of waste. Ensure the waste container is compatible with the
waste you are collecting.
• label the waste residue container with the appropriate waste label.
• store waste in a suitable area prior to collection. For example, chemicals and solvents
should be stored in ventilated areas and residue container lids must be secure. Ensure
container is not leaking and no spillage on the exterior of the container. Primary container
should be placed in a suitable bund.
• handle waste only if you are aware of the hazards associated with the waste and
appropriate risk controls are used.
• dispose waste as per relevant UOW guidelines.
• record all disposal on Waste Tracking Log to ensure evidence of correct waste
management.
Waste disposal pathways
Risk-free (non-infectious waste)
Waste
At risk of infection
Solid
Pungent or sharp
Other liquids
Chemical
Regular trash can
HDPE containers
Decontamination/
neutralization
before elimination
Incineration
Yellow trash can
Waste disposal pathways
Case of chemical waste with limited volumes

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Biotech Chapter 7.pptx

  • 2. Pesticide  A pesticide is any substance or mixture of substances intended for preventing , destroying , repelling , or mitigating any pest.  Pest include insects, plant pathogens, weeds, birds, and microbes etc.
  • 3. Classification on the basis of Targeted Organism Pesticides Algicides Virucides Rodenticide Fungicides Herbicides insecticide Avicides Bactericide miticides nematicide
  • 5. Chemical Pesticides  1. Organochlorides, which have a high persistence in the environment of up to about 15 years (DDT, dieldrin and aldrin  2. Organophosphates, which have an intermediate persistence of several months (parathion, carbaryl and malathion)  3. Carbamates, which have a low persistence of around two weeks (Tenik, Zectran and Zineb)  4. Synthetic pyrethroids, which are nonpersistent, contact and residual acting insecticides (cypermethrin, permethrin), suitable for a wide range of crops and target insect
  • 6. Biopesticides  Biopesticides are certain type of pesticides derived from such natural materials as plants, animal and microbes like bacteria, fungi etc.
  • 7.
  • 8. Microbial Pesticides  Microbial pesticides are composed of microscopic living organisms (viruses, bacteria, fungi, protozoa, or nematodes) or toxin produced by these organisms  Applied as conventional insecticidal sprays, dusts, or granules.  Their greatest strength is their specificity as most are essentially nontoxic and non pathogenic to animals and humans.  Microbial pesticides includes insecticides, fungicides, herbicides and growth regulators of microbial origin.
  • 9. Some of the important microbial pesticides a. Bacillus thuringiensis  Discovered in Japan in early 20th century and first become a commercial product in France in 1938.  Control lepidopterous pests like American bollworm in cotton and stem borers in rice. Fig: Bacillus thuringiensis  When ingested by pest larvae, Bt releases toxins which damage the mid gut of the pest, eventually killing it.  Main sources for the production of Bt preparations are the strains of the subspecies kurstaki, galeriae and dendrolimus
  • 10. e. Metarizium anisopliae •It infects spittlegbugs, rhinoceros beetles. f. Beauveria bassiana • Controls Colorado potato beetle. g. Verticillum lecanii: •Controls aphids and whiteflies. h. Nomuraea riley: • Controls soybean caterpillars. i.Baculoviruses (Bvs) • Control lepidopterous and hymenopterous pests. •Rod shaped, circular double stranded super coiled DNA. Fig: Metarhizium anisopliae Fig: Beauveria
  • 11.  Pesticides derived from plants  Generally act in one of two ways  Contact poison  Stomach poison  About 250000 plant species evaluated  2121 useful in pest management  1005 exhibited insecticidal activity  384antifeedants  297 repellents  27 attractants  31 growth inhibiting properties
  • 12. Plant-incorporated- protectants (PIPs)  Pesticidal substances that plant produce from the genetic material that has been added to the plant.  As the pest feed on such plants they will eventually die. Botanical pesticides:  These are naturally occurring plant material that may be crude preparation of the plant parts ground to produce a dust or powder that can be used in full strength or dilute form in a carrier such as clay, talc or diatomaceous earth.  “Azadirachtin” effects the reproductive and digestive process of pest.  Several plant based insecticides as nicotinoids, natural pyrethroids, rotenoids, neem products etc are used.
  • 13. 4.Biochemical pesticides  They are naturally occurring substance to control pest by non-toxic mechanisms.  Biochemical pesticides include substances as insect sex pheromones, that interfere with mating that attract insect pest to traps.  The synthetic attractants- are used in one of four ways: i. As a lure in traps used to monitor pest populations; ii. As a lure in traps designed to “trap out” a pest population; iii. As a broadcast signal intended to disrupt insect mating iv. As an attractant in a bait containing an insecticide Fig: weevil pheromone trap
  • 14.  Biopesticides are usually inherently less toxic than conventional pesticides.  Biopesticides generally affect only the target pest and closely related organisms,  Biopesticides often decompose quickly, avoiding the pollution problems caused by conventional pesticides.  Biopesticides can greatly decrease the use of conventional pesticides, while crop yields remain high.  Cheap, renewable can be handled safely.  Difficult for insects to develop resistance to these pesticides.
  • 15.  Slow effect  Lack persistence and wide spectrum activity  Rapidly degraded by UV lights so residual action is slow.  Seasonal availability of plants products indicates the needs for storage.  They are not available easily  Poor water solubility and generally not systemic in nature  All products applied followed by growers have not been scientifically verified.
  • 16. Fate of Pesticides Once a pesticide is applied, several things may happen.  It may be taken up by plants and/or ingested by animals, insects, worms, or microorganisms in the soil  It may move downward in the soil and adhere to soil particles, or it may dissolve  It may volatilize  It may be broken down into less toxic compounds  It may be leached or moved out of the plants root zone by rain or irrigation water or  It may be carried away by runoff water or erosion
  • 17. Factors Affecting Fate Of Pesticides There are four major factors which affect the fate of pesticides. They are as follows: 1. Properties of the pesticide 2. Properties of the soil 3. Conditions of the site 4. Management practices
  • 19. Biosafety Levels • Biosafety: Containment principles, techniques and practices implemented to avoid unintentional exposure to pathogens or toxins or their accidental release. • Biosecurity : Protection, control and accountability for valuable biological materials in laboratories, in order to restrict access and avoid loss, theft, misuse, diversion or deliberate release
  • 20.  Biological materials:  Samples (blood, urine, tissue, secretions etc.) in common use in laboratories and research units are a source of risk sometimes identified but often unrecognized and difficult to assess  Microorganisms including those which are genetically modified, cell cultures and human endoblasts capable of causing infection, allergy or intoxication  Biological risk: Probability of being exposed to a biological hazard - Directly: agent triggering the disease - Indirectly: toxin
  • 21.  Biological agents are divided into 4 hazard groups:  By the pathogenicity of biological agents  By the risk of illness of the exposed person  By the risk of spread in the community  By the existence of a prophylaxis or an effective treatment
  • 22. Groups Characteristics Examples 1 Does not cause disease in humans. Lactobacillus, Adenovirus, E. coli… 2 Could cause illness in humans and be a danger to workers ; Their spread in the community is unlikely ; There is usually effective prophylaxis or treatment. Staphylococcus aureus, influenza virus, measles virus, Hepatitis virus A, B, C, E, Plasmodium… 3 Could cause serious illness in humans and pose a serious danger to workers ; Spread to the community is possible, but there is usually effective prophylaxis or treatment SARS-CoV-2, Yellow fever virus, Dengue virus, HIV, Rift valley fever virus, West Nile virus… 4 Cause serious illness in humans and pose a serious danger to workers ; The risk of their spread to the community is high ; There is usually no prophylaxis or effective treatment. Lassa virus, Ebola virus, Crime Congo virus … Classification of biological agents
  • 23. Facilities: Types of laboratory – Biosecurity level and corresponding Microbiological Safety Cabinets Containment level Local Specific equipment Good practices BSL1 Room ventilated and insulated by a door and closed windows. Easily washable benches, walls and floors. Autoclave in the building Protective clothing. Clean and tidy mats. GLP BSL2 BSL1 + Regulated access for authorized personnel , making of premises, hermetic closure for fumigation (optional), sinks with non- manual controls. Autoclave Microbiological safety cabinet (MSC) Secure centrifuges. PPE : Blouse, gloves, glasses, use of needle box, use of disposable materials, inactivation of contaminated material (alcohol 70 etc.) and waste BSL3 BSL2 + airlock, filtration of incoming and outgoing air, intercom (optional), negative pressure with alarm system, generator, shower (optional) MSC II, double entry autoclave Same layouts as in BSL2 + wearing overboots and overcoats BSL4 BSL3 + Emergency ventilation system, intercom compulsory, double airlock, shower compulsory MSC III Same layouts as in BSL3 + use a protective suit MSC I MSC II MSC III
  • 24. Workspace organization under MSC Clean area Work zone Contaminated area
  • 25. Facilities:  Appropriate size, structure and location  Space to avoid contamination of samples with separation of activities, limited access  Storage areas: controlled temperature, defined limits, alarm, backup  Good maintenance
  • 27. Waste disposal pathways • minimise waste and do not accumulate large amounts in the laboratory. Regular disposal from the laboratories must be part of the laboratory WHS program. • segregate waste - have a separate residue container if you are generating a large amount of any particular type of waste. Ensure the waste container is compatible with the waste you are collecting. • label the waste residue container with the appropriate waste label. • store waste in a suitable area prior to collection. For example, chemicals and solvents should be stored in ventilated areas and residue container lids must be secure. Ensure container is not leaking and no spillage on the exterior of the container. Primary container should be placed in a suitable bund. • handle waste only if you are aware of the hazards associated with the waste and appropriate risk controls are used. • dispose waste as per relevant UOW guidelines. • record all disposal on Waste Tracking Log to ensure evidence of correct waste management.
  • 28. Waste disposal pathways Risk-free (non-infectious waste) Waste At risk of infection Solid Pungent or sharp Other liquids Chemical Regular trash can HDPE containers Decontamination/ neutralization before elimination Incineration Yellow trash can
  • 29. Waste disposal pathways Case of chemical waste with limited volumes