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RACE 的简介 花卉生物工程研究所:许志茹 2004 、 03 、 13
RACE 技术的简介 ,[object Object],[object Object],[object Object]
RACE 的优点 ,[object Object],[object Object],[object Object],[object Object]
实验室现有的 RACE 试剂盒的简介 ,[object Object],[object Object]
RACE 引物的设计: ,[object Object],[object Object],[object Object],[object Object],[object Object]
反应中涉及到的一些事项 ,[object Object],[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
具体的实验步骤 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
接头的连接及连接产物的稀释 ,[object Object],[object Object]
RACE - PCR 扩增 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
RACE 产物的验证:   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
比较由 GSP 和 NGSP 获得 RACE 产物。 ,[object Object],[object Object],[object Object]
RACE 产物的克隆和测序: ,[object Object],[object Object],[object Object]
- ,[object Object],[object Object]
全长 cDNA 的获得 ,[object Object],[object Object],[object Object]
通过 PCR 的方法获得全长 cDNA : ,[object Object],[object Object]
通过 PCR 的方法获得全长 cDNA : ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
通过 PCR 的方法获得全长 cDNA : ,[object Object],[object Object],[object Object],[object Object]
通过 PCR 的方法获得全长 cDNA : ,[object Object],[object Object]
通过克隆产生全长的 cDNA :   ,[object Object],[object Object],[object Object]
Appendix : cDNA 接头和引物 ,[object Object],[object Object],[object Object]
[object Object]

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