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Evaluating Regional Projects &
Opportunities for PRRS Elimination
      Bob Morrison DVM, MBA, PhD
         University of Minnesota
                    &
             USDA PRRS CAP
“Voluntary, producer-led,
coordinated, regional disease
     control programs”
Voluntary, Producer-led
• All within the region will benefit,

            but

• Not all will participate.     “Producer-led”:

                                - Acceptance

                                - Patience

                                - Subtle persistence
PRRS CAP website
Cold Weather Biosecurity Practices: Preventing PRRSV
         PRRSV happens year around but it is during the cold weather when we see most of the
         infections. As we approach this cold season, remember the following:
            SUGGESTED RESPONSE GUIDE TO A PRRS OUTBREAK
          1. PRRSV likes it cold and humid.
PURPOSE: The purpose of this document is to encourage all partieswet andby a newbeen cleaned and
      PRRSV survives well on equipment that is still affected hasn’t
outbreak of PRRS to consider how he/she will respond to the outbreak in a way that will
satisfy and protect the best interest of all parties.

RESPONSE BY PRODUCER WHO CONTRACTS NEW PRRS OUTBREAK:
  1. Gather team to discuss diagnosis, possible OF DISCLOSURE OF PRRS POSITIVE SITE
                                         RISK source of infection, strategy for
     investigation, and expected implications. Consider including local veterinarian,
     consulting veterinarian, farm managers, key employees, banker/finance manager, prepare for the possibility
                        PURPOSE: The purpose of this document is to help producers
     and regional PRRS coordinator. Discuss disclosure arrangement and how to share
                        of a PRRS outbreak—to anticipate the variable and uncontrolled responses by
     results with regional elimination participants.
                         individuals, businesses and the community. No one can control or predict behavior in a
                         voluntary effort to eliminate a disease, but it is important for each producer to
                       PRRS OUTBREAK risk of disclosing a positive site.
                         contemplate the INVESTIGATION
                                     PROTOCOL

Objective: The purpose of this protocol is to assist the veterinarian and producer in
building a comprehensive case history with the ultimate goal of discovering the source of
a new PRRS infection.
N212 MN Regional PRRS Elimination
            Program

    Dave Wright, Montse Torremorell,
             Bob Morrison

Swine Health Center, Morris Vet Clinic, Nate
Winkelman, Swine Vet Center, Neil DeBuse,
       Fairmont Vet Clinic & others
N212MN Project
                         • N212MN funding
                           – ’04 – ’06 - none
• IACUC:                   – ’07 – ’09 - USDA
   – 0711A21864            – ‘10 – ’12 – PRRS CAP &
                             UMN SDEC
                         • Rice
                           – MPB
• Disclosure:
                           – BI PRRS Initiative
   – RBM - 2 sow farms
   in Stevens Cy           – AASV
Stevens County
Stevens County


                                  2011


       2004


Reduced infected farms from
31 in 2004 to none known in
     2012 (5.7% of sites
         unknown)
First Expansion
Second Expansion
Identified sites in the region
•   251 negative sites
•    46 positive sites
•   409 unknown sites
•   706 sites identified

• 315 have signed the participation agreement
Morris vets
Al Carlson
                                      Nate Winkelman




                                  Swine Vet Center




   Has sows                negative

   Growing pigs, no sows   unknown
                           positive
Sow herds in the region
                   (& nurseries & finishers)
                                         Sow herd size
                       <=100   101-600      601-1500     1501-3000   >3000
Farrow to wean          1        1            13            12          11
Farrow to feeder                 1
Farrow to finish        23       11


                                         Sow herd size
                       <=100   101-600      601-1500     1501-3000   >3000
Unknown                 17       5             8            1            2
Positive                                       1            1            1
Positive stable
Negative provisional
Negative                7        8             4            10           8
ADMINISTRATIVE CHALLENGES
• Site identification

• Managing data base and maps

• Notification of status changes

• Relying on voluntary disclosure for herd status
  updates
SOCIAL CHALLENGES
• Encouraging participation

• Weighing individual farm interest with region’s
  interest

• Managing confidentiality and risk of disclosure
Managing Disclosure
• Knowing the neighbor’s PRRS status is a
  privilege.
• Pressuring the neighbor will drive it
  underground.

• “Thank you for telling me. I’m sorry this is
  happening to you. Do you mind telling me
  your plans? Is there anything I can do to
  help?”
Update for NE IL:
• Like many other areas, we had a quick rash of breaks in mid
  fall, but have been able to contain them fairly well. All of
  those producers are in the midst of control programs, and will
  or have started elimination plans (Load-Close-
  Homegenize). One sow farm has decided to go ahead and
  filter the farm. No new strain introductions this year.

• The mood continues to be one of “Yes, We Can”; however, it is
  disheartening on all sides (vets & producers) when breaks on
  farms, that have eliminated virus and were having
  phenomenal production, occur. Everyone is still committed to
  the end goal: controlling & eliminating field-strain PRRS from
  our area.
NC Illinois
Region             North-Central IL                  Density sows: 27.8/sq. mile
Year / Quarter     Q4 2011, as of Dec. 31, 2011      Density pigs: 194.6/sq. mile

                                             Sow herd size
                    <=100      101-600           601-1500       1501-3000     >3000
Farrow to wean                                      1               4           3
Farrow to feeder                                    1               2
Farrow to finish      2          7                  3                           1
TOTAL                 2          7                  5               6           4     24

                                         Sow herd Status by Size
                    <=100      101-600           601-1500       1501-3000     >3000
Unknown              1            1                                             1
Positive                         4**               2***            4****        1
Positive stable      1            2                 1               1           2
Negative provisional                                1
Negative                                            1               1
TOTAL                2           7                  5               6           4     24
30

25

20
                                               NEG
15                                             NEG PROV
                                               POS-STABLE
10                                             POS
                                               UNK
5

0
     2010 2010 2010 2010 2011 2011 2011 2011
      Q1   Q2   Q3   Q4   Q1   Q2   Q3   Q4
Nurseries (no sows or pigs >10 wks)
                         <=1200     1201-2400       2401-4800       >4800
                         Source       Source          Source        Source
                        IN     OUT    IN     OUT     IN      OUT     IN     OUT

         Unknown
          Positive      1*            1*             3^
          Negative
             Total      1      0      1      0       3       0       0      0     5

                                     Finishing or Wean to finish
                         <=1200      1201-2400      2401-4800         >4800
                         Source        Source         Source          Source
                        IN     OUT    IN     OUT     IN      OUT     IN     OUT

       Unknown                        1              1                               2
         Positive    9*       3*     8^^     1*     8^^^     4**   10^^^^           43
         Negative                     2      1       1               3               7
            Total     9       3      11      2      10       4      13      0     52
24 sows, 5 nurseries, 52 finishers

* - Positives are MLV strain
** - 2 of 4 are MLV strain
*** - 1 of 2 are MLV strain
**** - 3 of 4 are MLV strain
80

70

60

50
                                                                       NEG
40                                                                     POS-MLV
                                                                       POS
30
                                                                       UNK
20

10

0
     Q1 2010 Q2 2010 Q3 2010 Q4 2010 Q1 2011 Q2 2011 Q3 2011 Q4 2011
Current Needs/Challenges:
• Manpower – there never seems to be a shortage of things
  we want to do for this area: educational
  programs, improved biosecurity trainings, enhanced testing
• Endurance – the original “push” seems to have faded, and
  the challenge will continue to be staying focused &
  motivated in the years ahead
• Coordination Among Projects – now that there are so many
  projects throughout the US, there needs to be a focused
  effort (by NPB, AASV?) to connect the
  leaders, coordinators, and participants of these projects so
  that they can learn from each other, work in tandem with
  one another (projects now know pig flows not only in & out
  of their area, but in & out of other project areas).
NE project
Cuming Co, NE
1) Approx 10 new sow breaks & rumors of problems in some finishers
that haven't been checked yet. These farrowing units provide pigs to
about 25-30 grow finish sites so we are trying to get to all of the sites
to see if offspring is positive. Primarily reproductive problems at sow
units with high number of parasuis cases in newly positive nurseries.

2) The mood is pretty good. The project still has good momentum
although it does come in spurts. It seems if I have enough time to get
out to producers with project they are really excited. We are trying
some MJ prrs in a couple of our areas.

3) The current needs of the project continue to be that of time to
devote to it. When it is rolling we can accomplish a lot in a little bit of
time but it does get bogged down from time to time.
PRRS Area Regional Control Project
        Iowa County, Iowa
          Derald Holtkamp, DVM, MS
              Iowa State University
         College of Veterinary Medicine




                                          IVMA Winter Conference
                                             February 8, 2012
Project Area




• West and north of Highway 151 to Highway 6
  to Highway 149 as it bisects the county
• All sites with commercial pigs
Swine Premises in Iowa County
Iowa County
Region                 Iowa County             Density sows:     10.28 per sq mile
Date                   1/24/2012               Density pigs:     186.89 per sq mile

                                                 Sow herd size
                        <=100        101-600       601-1500           1501-3000       >3000
Farrow to wean                          1              1                  1
Farrow to feeder
Farrow to finish
Show
Unknown                    2           5

                                                 Sow herd size
                        <=100        101-600       601-1500           1501-3000       >3000
Unknown
Positive                               2                                  1
Positive stable                        1               1
Negative provisional
Negative                   2           3
Iowa County
                             Sow herd status
12


10


8
                                                       Negative
                                                       Negative provisional
6
                                                       Positive stable

4                                                      Positive
                                                       Unknown
2


0
     2010 Q3 2010 Q4 2011 Q1 2011 Q2 2011 Q3 2011 Q4
Nurseries (no sows or pigs >10 wks)
                            <=1200                1201-2400           2401-4800                 >4800
                            Source                 Source              Source                  Source
                       inside   outside       inside    outside   inside    outside       inside    outside
                       region    region       region    region    region    region        region    region
           Unknown
            Positive                      1                                           1
     Positive stable
Negative provisional
           Negative                       1                                           1
               Total                      2                                           2

                                                   Finishing or Wean to finish
                            <=1200                1201-2400           2401-4800                 >4800
                            Source                 Source              Source                  Source
                       inside   outside       inside    outside   inside    outside       inside    outside
                       region    region       region    region    region     region       region    region
           Unknown
            Positive                                  3 (2 finishers by vaccine)
                                                                           1          1
     Positive stable
Negative provisional
           Negative
               Total                                           3           1          1
Iowa County
• Substantial progress in 2011.
• Only 2 incidents of PRRS.
  – A nursery that receives pigs from an outside
    source. Sequence matches source’s strain.
  – A wean-to-finish site that had been negative since
    the beginning of the area project, but in
    November was positive. Source is unknown.
Some highlights:
• Dr. Aljets presented at the Swine Disease Conference at
  Iowa State in November 2011
• A project vet requested a feed truck delivery pyramid
  be drafted the project area. Feed truck biosecurity
  facts and tips were drafted and will be shared with the
  producers.
• Project dendogram continues to be built.
• Monthly updates continue to be sent to producers and
  veterinarians which contain data tables, maps and
  other appropriate updates
• PADRAPs are all but complete, only 2 more need to be
  completed
What’s next?
• Herd plans
• Expanded the project boundaries to include all of Iowa
  County commercial producers.
   – Many producers in the eastern portion of the county are
     beginning to get involved with the Washington County
     project and sharing their information, & would be willing
     to become a part of the Iowa County Project.
   – Many sow farms in eastern portion of the county or
     Washington county finish pigs in our current project area
     or vice versa. If we were to expand the project, producers
     and veterinarians believe that we could get a more
     complete picture of pig and virus movement in the county.
Current needs & challenges:
• Keep producers focused and motivated. To encourage the project
  onwards we plan to hold biosecurity workshops, bring in quest
  speakers at quarterly meetings and explore other options. Some
  producers are not excited to be retested. Leaders of project, along
  with vets are encouraging producers to keep monitoring sites, as
  well as complete PADRAPs and herd plans.

• Positive pigs are being placed in project area. If we could get
  negative pigs coming into area that would automatically clean up
  many growing pig sites.

• Better biosecurity. We are focusing on better biosecurity practices
  including feed truck delivery biosecurity and transporting market
  hogs. Transporting market hogs was the main focus of the last
  producer meeting.
“Voluntary, producer-
led, coordinated, regional disease
         control programs”
Current Issues & Workgroups:
• 7 working groups

• Failure of some closure programs

• Risk factors for spread in hog dense regions
Seven working groups:
1. Standard Of Practice for sow herd management &
   sampling
2. Minimum sampling guidelines for low risk sow herds
   and growing pig sites
3. Risk based sampling
4. Incorporating oral fluids into sampling-testing
   guidelines
5. Managing the risk of disclosure
6. Farrow to finish herds
7. Guidelines for implementing common
   reports, including mapping legends
Evaluating Regional Projects &
Opportunities for PRRS Elimination
PRRS SURVIVABILITY STUDIES

         Condition                            Length of Survival

          Frozen                                      Years

     Moist, Cold, Wet                                11 days

       700 F (210 C)                                  6 days

      98.6 0 F (37o C)                               24 hours

       132o F (56o C)                            20 minutes



Pitkin, Otake, Dee, “Biosecurity protocols for the
prevention of spread of PRRSv”, Swine Disease
Eradication Center, University of Minnesota

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Dr. Robert Morrison - Evaluating Regional Projects and Opportunities for PRRS Elimination

  • 1. Evaluating Regional Projects & Opportunities for PRRS Elimination Bob Morrison DVM, MBA, PhD University of Minnesota & USDA PRRS CAP
  • 3. Voluntary, Producer-led • All within the region will benefit, but • Not all will participate. “Producer-led”: - Acceptance - Patience - Subtle persistence
  • 4.
  • 6. Cold Weather Biosecurity Practices: Preventing PRRSV PRRSV happens year around but it is during the cold weather when we see most of the infections. As we approach this cold season, remember the following: SUGGESTED RESPONSE GUIDE TO A PRRS OUTBREAK 1. PRRSV likes it cold and humid. PURPOSE: The purpose of this document is to encourage all partieswet andby a newbeen cleaned and PRRSV survives well on equipment that is still affected hasn’t outbreak of PRRS to consider how he/she will respond to the outbreak in a way that will satisfy and protect the best interest of all parties. RESPONSE BY PRODUCER WHO CONTRACTS NEW PRRS OUTBREAK: 1. Gather team to discuss diagnosis, possible OF DISCLOSURE OF PRRS POSITIVE SITE RISK source of infection, strategy for investigation, and expected implications. Consider including local veterinarian, consulting veterinarian, farm managers, key employees, banker/finance manager, prepare for the possibility PURPOSE: The purpose of this document is to help producers and regional PRRS coordinator. Discuss disclosure arrangement and how to share of a PRRS outbreak—to anticipate the variable and uncontrolled responses by results with regional elimination participants. individuals, businesses and the community. No one can control or predict behavior in a voluntary effort to eliminate a disease, but it is important for each producer to PRRS OUTBREAK risk of disclosing a positive site. contemplate the INVESTIGATION PROTOCOL Objective: The purpose of this protocol is to assist the veterinarian and producer in building a comprehensive case history with the ultimate goal of discovering the source of a new PRRS infection.
  • 7.
  • 8. N212 MN Regional PRRS Elimination Program Dave Wright, Montse Torremorell, Bob Morrison Swine Health Center, Morris Vet Clinic, Nate Winkelman, Swine Vet Center, Neil DeBuse, Fairmont Vet Clinic & others
  • 9. N212MN Project • N212MN funding – ’04 – ’06 - none • IACUC: – ’07 – ’09 - USDA – 0711A21864 – ‘10 – ’12 – PRRS CAP & UMN SDEC • Rice – MPB • Disclosure: – BI PRRS Initiative – RBM - 2 sow farms in Stevens Cy – AASV
  • 11. Stevens County 2011 2004 Reduced infected farms from 31 in 2004 to none known in 2012 (5.7% of sites unknown)
  • 14. Identified sites in the region • 251 negative sites • 46 positive sites • 409 unknown sites • 706 sites identified • 315 have signed the participation agreement
  • 15.
  • 16. Morris vets Al Carlson Nate Winkelman Swine Vet Center Has sows negative Growing pigs, no sows unknown positive
  • 17.
  • 18. Sow herds in the region (& nurseries & finishers) Sow herd size <=100 101-600 601-1500 1501-3000 >3000 Farrow to wean 1 1 13 12 11 Farrow to feeder 1 Farrow to finish 23 11 Sow herd size <=100 101-600 601-1500 1501-3000 >3000 Unknown 17 5 8 1 2 Positive 1 1 1 Positive stable Negative provisional Negative 7 8 4 10 8
  • 19.
  • 20. ADMINISTRATIVE CHALLENGES • Site identification • Managing data base and maps • Notification of status changes • Relying on voluntary disclosure for herd status updates
  • 21. SOCIAL CHALLENGES • Encouraging participation • Weighing individual farm interest with region’s interest • Managing confidentiality and risk of disclosure
  • 22. Managing Disclosure • Knowing the neighbor’s PRRS status is a privilege. • Pressuring the neighbor will drive it underground. • “Thank you for telling me. I’m sorry this is happening to you. Do you mind telling me your plans? Is there anything I can do to help?”
  • 23.
  • 24.
  • 25. Update for NE IL: • Like many other areas, we had a quick rash of breaks in mid fall, but have been able to contain them fairly well. All of those producers are in the midst of control programs, and will or have started elimination plans (Load-Close- Homegenize). One sow farm has decided to go ahead and filter the farm. No new strain introductions this year. • The mood continues to be one of “Yes, We Can”; however, it is disheartening on all sides (vets & producers) when breaks on farms, that have eliminated virus and were having phenomenal production, occur. Everyone is still committed to the end goal: controlling & eliminating field-strain PRRS from our area.
  • 26. NC Illinois Region North-Central IL Density sows: 27.8/sq. mile Year / Quarter Q4 2011, as of Dec. 31, 2011 Density pigs: 194.6/sq. mile Sow herd size <=100 101-600 601-1500 1501-3000 >3000 Farrow to wean 1 4 3 Farrow to feeder 1 2 Farrow to finish 2 7 3 1 TOTAL 2 7 5 6 4 24 Sow herd Status by Size <=100 101-600 601-1500 1501-3000 >3000 Unknown 1 1 1 Positive 4** 2*** 4**** 1 Positive stable 1 2 1 1 2 Negative provisional 1 Negative 1 1 TOTAL 2 7 5 6 4 24
  • 27. 30 25 20 NEG 15 NEG PROV POS-STABLE 10 POS UNK 5 0 2010 2010 2010 2010 2011 2011 2011 2011 Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4
  • 28. Nurseries (no sows or pigs >10 wks) <=1200 1201-2400 2401-4800 >4800 Source Source Source Source IN OUT IN OUT IN OUT IN OUT Unknown Positive 1* 1* 3^ Negative Total 1 0 1 0 3 0 0 0 5 Finishing or Wean to finish <=1200 1201-2400 2401-4800 >4800 Source Source Source Source IN OUT IN OUT IN OUT IN OUT Unknown 1 1 2 Positive 9* 3* 8^^ 1* 8^^^ 4** 10^^^^ 43 Negative 2 1 1 3 7 Total 9 3 11 2 10 4 13 0 52 24 sows, 5 nurseries, 52 finishers * - Positives are MLV strain ** - 2 of 4 are MLV strain *** - 1 of 2 are MLV strain **** - 3 of 4 are MLV strain
  • 29. 80 70 60 50 NEG 40 POS-MLV POS 30 UNK 20 10 0 Q1 2010 Q2 2010 Q3 2010 Q4 2010 Q1 2011 Q2 2011 Q3 2011 Q4 2011
  • 30. Current Needs/Challenges: • Manpower – there never seems to be a shortage of things we want to do for this area: educational programs, improved biosecurity trainings, enhanced testing • Endurance – the original “push” seems to have faded, and the challenge will continue to be staying focused & motivated in the years ahead • Coordination Among Projects – now that there are so many projects throughout the US, there needs to be a focused effort (by NPB, AASV?) to connect the leaders, coordinators, and participants of these projects so that they can learn from each other, work in tandem with one another (projects now know pig flows not only in & out of their area, but in & out of other project areas).
  • 32.
  • 33. Cuming Co, NE 1) Approx 10 new sow breaks & rumors of problems in some finishers that haven't been checked yet. These farrowing units provide pigs to about 25-30 grow finish sites so we are trying to get to all of the sites to see if offspring is positive. Primarily reproductive problems at sow units with high number of parasuis cases in newly positive nurseries. 2) The mood is pretty good. The project still has good momentum although it does come in spurts. It seems if I have enough time to get out to producers with project they are really excited. We are trying some MJ prrs in a couple of our areas. 3) The current needs of the project continue to be that of time to devote to it. When it is rolling we can accomplish a lot in a little bit of time but it does get bogged down from time to time.
  • 34. PRRS Area Regional Control Project Iowa County, Iowa Derald Holtkamp, DVM, MS Iowa State University College of Veterinary Medicine IVMA Winter Conference February 8, 2012
  • 35. Project Area • West and north of Highway 151 to Highway 6 to Highway 149 as it bisects the county • All sites with commercial pigs
  • 36. Swine Premises in Iowa County
  • 37. Iowa County Region Iowa County Density sows: 10.28 per sq mile Date 1/24/2012 Density pigs: 186.89 per sq mile Sow herd size <=100 101-600 601-1500 1501-3000 >3000 Farrow to wean 1 1 1 Farrow to feeder Farrow to finish Show Unknown 2 5 Sow herd size <=100 101-600 601-1500 1501-3000 >3000 Unknown Positive 2 1 Positive stable 1 1 Negative provisional Negative 2 3
  • 38. Iowa County Sow herd status 12 10 8 Negative Negative provisional 6 Positive stable 4 Positive Unknown 2 0 2010 Q3 2010 Q4 2011 Q1 2011 Q2 2011 Q3 2011 Q4
  • 39. Nurseries (no sows or pigs >10 wks) <=1200 1201-2400 2401-4800 >4800 Source Source Source Source inside outside inside outside inside outside inside outside region region region region region region region region Unknown Positive 1 1 Positive stable Negative provisional Negative 1 1 Total 2 2 Finishing or Wean to finish <=1200 1201-2400 2401-4800 >4800 Source Source Source Source inside outside inside outside inside outside inside outside region region region region region region region region Unknown Positive 3 (2 finishers by vaccine) 1 1 Positive stable Negative provisional Negative Total 3 1 1
  • 40. Iowa County • Substantial progress in 2011. • Only 2 incidents of PRRS. – A nursery that receives pigs from an outside source. Sequence matches source’s strain. – A wean-to-finish site that had been negative since the beginning of the area project, but in November was positive. Source is unknown.
  • 41. Some highlights: • Dr. Aljets presented at the Swine Disease Conference at Iowa State in November 2011 • A project vet requested a feed truck delivery pyramid be drafted the project area. Feed truck biosecurity facts and tips were drafted and will be shared with the producers. • Project dendogram continues to be built. • Monthly updates continue to be sent to producers and veterinarians which contain data tables, maps and other appropriate updates • PADRAPs are all but complete, only 2 more need to be completed
  • 42. What’s next? • Herd plans • Expanded the project boundaries to include all of Iowa County commercial producers. – Many producers in the eastern portion of the county are beginning to get involved with the Washington County project and sharing their information, & would be willing to become a part of the Iowa County Project. – Many sow farms in eastern portion of the county or Washington county finish pigs in our current project area or vice versa. If we were to expand the project, producers and veterinarians believe that we could get a more complete picture of pig and virus movement in the county.
  • 43. Current needs & challenges: • Keep producers focused and motivated. To encourage the project onwards we plan to hold biosecurity workshops, bring in quest speakers at quarterly meetings and explore other options. Some producers are not excited to be retested. Leaders of project, along with vets are encouraging producers to keep monitoring sites, as well as complete PADRAPs and herd plans. • Positive pigs are being placed in project area. If we could get negative pigs coming into area that would automatically clean up many growing pig sites. • Better biosecurity. We are focusing on better biosecurity practices including feed truck delivery biosecurity and transporting market hogs. Transporting market hogs was the main focus of the last producer meeting.
  • 44. “Voluntary, producer- led, coordinated, regional disease control programs”
  • 45. Current Issues & Workgroups: • 7 working groups • Failure of some closure programs • Risk factors for spread in hog dense regions
  • 46. Seven working groups: 1. Standard Of Practice for sow herd management & sampling 2. Minimum sampling guidelines for low risk sow herds and growing pig sites 3. Risk based sampling 4. Incorporating oral fluids into sampling-testing guidelines 5. Managing the risk of disclosure 6. Farrow to finish herds 7. Guidelines for implementing common reports, including mapping legends
  • 47. Evaluating Regional Projects & Opportunities for PRRS Elimination
  • 48.
  • 49. PRRS SURVIVABILITY STUDIES Condition Length of Survival Frozen Years Moist, Cold, Wet 11 days 700 F (210 C) 6 days 98.6 0 F (37o C) 24 hours 132o F (56o C) 20 minutes Pitkin, Otake, Dee, “Biosecurity protocols for the prevention of spread of PRRSv”, Swine Disease Eradication Center, University of Minnesota