Simtronics Analytical And Lab Instruments Haryana India

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We are manufacturer, supplier and exporter of scientific & laboratory testing and measuring instruments. Our products are extensively used in pharmaceutical & direct & indirect export projects and engineering colleges.

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“ Manufacturer & Exporter of Scientific & Laboratory Testing & Measuring Instruments” Simtronics Analytical & Lab Instruments

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A spectrophotometer is a photometer that can measure the intensity of light as a function of its wavelength. Single beam and double beam are the two major classes of spectrophotometers. Linear range of absorption and spectral bandwidth measurement are the important features of spectrophotometers. In Single Beam Spectrophotometers, all the light passes through the sample. To measure the intensity of the incident light the sample must be removed so that all the light can pass through. This type of spectrometer is usually less expensive and less complicated. The single beam instruments are optically simpler and more compact, znc can also have a larger dynamic range. In a Double Beam Spectrophotometer, before it reaches the sample, the light source is split into two separate beams. One beam passes through the sample and the second one is used for reference. This gives an advantage because the reference reading and sample reading can take place at the same time. In transmission measurements, the spectrophotometer quantitatively compares the amount of light passing through the reference and test sample. For reflectance, it compares the amount of light reflecting from the test and reference sample solutions. Many spectrophotometers must be calibrated before they start to analyse the sample and the procedure for calibrating spectrophotometer is known as "zeroing." Calibration is done by using the reference substance, and the absorbencies of all other substances are measured relative to the reference substance. % transmissivity (the amount of light transmitted through the substance relative to the initial substance) is displayed on the spectrophotometer.

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