Use of animations disturbs the view. For full ppt, drop me a mail at varadbende96@gmail.com

3. HELLO!
Varad Bende
TE Biotech
Department of Biotechnology
Jawaharlal Nehru Engineering College , Aurangabad MS 431003
Let us put a glance over
Columns used in HPLC.

4. High Performance
Liquid Chromatography
(HPLC)

5. • The Brain of HPLC
• Resolves a mixture into its
different components
• Available in different material
packings and sizes according
to the requirements

6. Normal Phase
Chromatography
Reverse Phase
Chromatography

7. Analyte of Interest
• Chemical Properties
• Physical Properties
Type of chromatography
Purpose of chromatography
Amount of Sample
Precision needed

8.  Advanced Chromatography
Technologies
 Agela Technologies
 Agilent
 Applied
 Biosystems
 Beckman Coulter
 Bio-Rad
 Biotage
 ChiralTech
 Eksigent
 ES Industries
 Grace Discovery Sciences
 Hamilton
 Imtakt
 J & K Scientific
 Kromasil
 Life Technologies
 MAC-MOD
 Peeke Scientific
 PerkinElmer
 Phenomenex
 Restek
 Shiseido
 Shodex
 Sigma-Aldrich
 SiliCycle
 Thermo Fisher Scientific
 Tosoh Bioscience
 Valco
 Waters
 Zirchrom
(Reference - www.labmanager.com)

9. Particle Size 2.5 to 10 μm 2.5 , 3.5 , 5 μm 2.7 μm
Packing
Material
C4 C8 C18 Phenyl Amide C18 Cyano PFP T3 Phenyl C18+ C18 T3 C8
Technology Ethylene Bridged Hybrid • Charged Surface
Hybrid(CSH)
• High Strength Silica (HSS)
Solid Core Technology
Dimensions 75μm*5mm to 30*300 mm 1*5 mm to 50*250 mm 2..1*30 mm to 4.6*150 mm
Cost ₹ 43,500 to 1,90,000 ₹ 34,000 to 11,00,000 ₹ 32,000 to 76,000
Specificatios • Analytical and Preparative
• Robust
• High pH stability (1-12)
• BioSeparation
• Analytical and Preparative
• Reverse phase
• Designed for selectivity
• Highest analyte mass
loading
• High efficiency
• Reverse Phase
• Compatible with UPLC

12. Infinity Lab
•2.1 * 50 mm to 4.6 * 250 mm
•Particle Size 4 μm
•Cost ₹ 20,000 to 1,00,000
•Packing Materials - C8 C18 PhenylHexyl
•Long life
• 2.1 * 50 mm to 4.6 * 250 mm
• Particle Size 3.5 and 5 μm
• Cost ₹ 20,000 to 1,00,000
• Packing Materials - C8 C18 PhenylHexyl PAH
• Excellent chromatography with mixtures of polar and nonpolar analytes
• Particle Size 3.5 and 5 μm
• Cost ₹ 24,000 to 56,000
• Packing Materials - C8 C18 PhenylHexyl

14.  Material Packings - C8 C18 Amide
Cyano Silica
 Particle size – 2.7 – 5μm
 Cost 26,000 to 3,00,000
 High purity silica
 Modern bonding processes
 Analytical and Preparative

15. TM
• Packing Material – C5 C8 C18
• Particle Size – 3-5 um
• Cost - 30,000 to 50,000
• Aqueous compatible

16. SupelcoSIL TM
• Packing Material – C8 C18 Phenyl
• Particle size – 40,000 to 70,000
• Silica Based
• Micro to Preparative scale
• High Quality

18. • Ultra-pure silica-based HPLC columns
• Particle Size - 3 to 5μm
• Available in C4 C5 C8 C18 PhenylHexyl Silica CN Amino
• Cost – 5,000 to 50,000
• Wide pH stability for long column lifetime and method flexibility
• Easy method scalability from Fast LC to Preparative and Bulk
Purification

19. • Uses Core Shell Technology along with Ethane
Cross Linking
• 2.6 , 4 , 5μm
• C8 C18 Polar
• Analytical and preparative
• Aqueous Stability
• Improved peak shapes

20. • Accucore
• Acclaim
• BetaBasic
• BioBasic
• Hypersil BDS
• Hypersil GOLD
• Syncronis
• Hypercarb
• HyperRez
• HyPURITY

21. Accucore™ LC Columns
Particle Size - 2.6µm solid-core particles
Material - C8 C18 PhenylHexyl C30 etc
Dimensions - 2 * 50 mm to 4.6 * 150 mm
Price - 20,000 to 50,000
Synchronis™ LC Columns
• Highly pure, high surface area silica
• Dense bonding and double endcapping
• Stringent manufacturing and testing protocols-
manufactured, packaged and tested in ISO9000
accredited facilities
Columns: C18, C8, Phenyl, Amino, Silica
Acclaim™ LCColumns
Particle Size - 2.2µm Porous silica particles with
advanced and innovative column bonding technologies
Material - C8 C18 PhenylHexyl C30 etc
Dimensions - 2 * 50 mm to 4.6 * 150 mm
Price - 20,000 to 50,000

23. • Hybrid Silica based HPLC Columns
• Multi-layered, organic/inorganic hybrid
particle
• Double Endcapped
• Wider pH and Temperature Range
• Material - C18 C8 Phenyl Silica
• Particle Size - 3 , 5 , 10 , 50μm
• Dimensions – 2*20 mm to 4.6*250 mm
(30*250 mm in Preparative)
• Cost – 20,000 to 50,000 (Upto 10,00,000 in
Preparative)

24. CoreShell Columns by YMC
• Particle Size – 2.7μm
• Dimensions - 2.6*40 to 4.6*150
• Material - C8 and C18
• Cost - 21,000 to 40,000
• Coreshell Technology
• Ultra fast separation with outstanding
resolution
• Excellent peak shape for basic and
coordination compounds
• Wide pH application range
• Low column bleed, ideal for LC/MS

25. YMC Pro Columns
• Particle size - 2 , 3 , 4μm
• Materials - C8 C18
• High performance , reliable columns
designed for the most demanded HPLC
• Novel lewis acid/lewis base endcapping
• Sharp and symmetrical peaks

26. Analyte of Interest
• Chemical Properties
• Physical Properties
Type of chromatography
Purpose of chromatography
Amount of Sample
Precision needed

28. • Waters
• Agilent
• Phenomenex
• Sigma Aldrich
• GE Life Sciences
• Altech
• Shimadzu
• Fisher Scientific
• YMC
• Perkin Elmer
(Reference – www.labx.com)

29. YMC, Japan
• Waters
• ThermoFisher
Massachusetts USA• Phenomenex
• Agilent
California USA
GE Healthcare UK
Sigma Aldrich
Missouri USA
ACE Scottland

30. PREPARATIVE
CHROMATOGRAPHY

32. www.labmanager.com
Web Resources
 www.labmanager.com
 www.labx.com
 www.waters.com
 www.crawfordscientific-secure.com
 http://www.agilent.com
 www.thermofisher.com
 www.ymc.co.jp
 www.phenomenex.com
 www.sigmaaldrich.com

33. THANKS!
Any questions?
You can find me at
» varad93@rediffmail.com