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USE OF
BIOTECHNOLOGY FOR
CLEANING UP OUR
ENVIRONMENT
INTRODUCTION
Biotechnoligy deals with tecnioques of
using live organisms or enzymes from
organisms to produce products and process
useful to humans. Biotecnology,the 20th
centuryoff-shoot of modern
biology,changed our daily life as its
products brought qualitative improvement
in health and food products. The application
of biotechnology has helped in the
environmental managementof hazardous
contaminants by bioremediation.
Bioremediation involves the use of naturally
existing microorganisms to speed up the
breaking down of biological substances and
degradation of various material.
USE OF BIOTECHNOLOGY
Some of areas where biotechnology was
proved very effective in environmental clean-
up include;
Landfill Technologies;Solid wastes account for
an increasing proportion of the waste
generated by carbon societies. While a part of
this volume consist of a glass,plastics and
other non-biodegradable material
aconsiderable proportion of this made of
decompsible solid organic material like food
wastes from large poultery and pig farm.In
large non-urbanized communities ,a common
method for disposing off such biodegradble
waste is the low-cost Anaerobic landfill
technology . In this process ‘the solid waste
are deposited in low-lying,low valve sites.
COMPOSTING;Composting is an anaerobic
microbially driven process that converts organic
wastes into stable sanitary humus like material
.This material can than be safely returned to the
natural environment . This method is actually a
low moisture ,solid substrate fermentation
process.
The primary aim of a composting operation is to
obtain final compost with a desired product quality
in a limited time period and within limited
compost. The basic biological reaction of the
composting process is the oxidation of mixed
organic substrates to products CO2 and water and
other organic by-products. It is important to ensure
that a composting plant function under
environmently,safe conditions.
Bioremediation;Various products generated by
the modern technoiogies are posing a great
threat tothe natural breakdown process and
the natural mechanisms to maintaining
ecological balance . Many of the pollutants are
complex in nature and arehence difficult to
breakdown. Such pollutants are accumulating
in natural environment to an alarming rate.
The application of biotechnology has heiped in
the environmental management of such
hazardous control by bioremediation.This
process is also reffered as bio-treatment.
Bioremediation involves the use of naturally
existing microorganisms to speed up the
breaking down of biological substances and
degradation of various material.
BIOSENSORS;Biosensors are biophysical
devices that can detect and measures the
quantities of specific subject in vareity of
environments. Biosensors include
enzymes’antibodies and even
microorganisms and these can be used for
clinical,immunological,genetics and other
research purposes. The biosensors probes
are used to detect and monitor pollutants
in the environment. These biosensors are
non destructive in nature ,and can utilise
whole cell or a specific molecules like
enzymes as bioremetic detection. Their
other advantages include rapid
analysis,specificity and accurate
reprodutibility.
OIL EATING BUGS;Accidental oil spills pose
great threat to ocean environments .such
spills have a direct impact marine
organisms. To encounter his problem,
scientists have know develpoed
livingorganisms to clean up the oil spills.
The most common oil eating
microorganisms are bacteria and fungi.
Pencillium species has also been found to
possess degrading features,but its effect
needs much more than the genetically
engeenered bacterium. Many other
microorganisms like the Alcanivorax
bacteria are also capable of degrading
petroleum products.
Designer Bugs:More than hundred
thousand different chemical compounds
are produced in the world every year.
While some of these chemicals are
degradable,others like chlorinated
compounds are resistant to microbial
degradation.
Biomining:Among the oldest industries in
the world,mining is the source of alarming
level of environmental pollution. Modern
biotechnology is now being used to
improve the environment surronding
mining area through various
microorganisms. A bactirium Thiobacillus
ferroxidans has been used to back out
copper from mine tailings. This bactirium is
naturally present in certain sulphur
containing materials,and can be used to
oxidise inorganic compounds like copper
sulfide minarals. This process releases
acids and oxidising solutions of ferric irons
that can wash out metals from the crude
ore. These bacteria chew up the ore and
release copper that can subsequently be
collected.
Pollution control: With the help of modern
biotechnology,naturally occuring
biocatalysts can be used to detoxify
harmful chemicals being released into the
environment such biocatalysts have helped
get rid of carcinogenic compounds like
methylene chloride from industrial waste.
These speacial bacteria are exposed to the
waste in a bioreactor,where in the bacteria
consume the noxious chemical and convert
it into water,co2 and salts,thus completly
destroying the chemical compound. A
species of bacteria Geobacter
metallireduces is also reffered to remove
uranium from drainage water in mining
operations,and from contaminated ground
waters.
The isolation and subsequent
characterisation of various important
genes will help in developing strains that
can degrade a wide range of pollutants.
Using molecular manipulations can also
help tailor bacteria to use them for
removing specific toxicants.
Treating industrial wastes:Wastes from the
paper and pulp industries contain high
levels of cellulose and ligno cellulose,which
pose massive treatment problems.
Cellulose is extremely resistant to enzyme
breakdown,and becomes resistant to both
chemical and enzymatic attack when
bound to lignin. Since lignin’s and
carbohydrates are interlinked in woods,it
becomes difficult to delignify the pulp.
Disposal is now being handheld by
various biotechnology approaches. These
include:
1) Treating whey with proper
strains of microbers and nutrients.
2) Direct fermentation of
lactose to ethanol.
3) Hydrolysis of lactose to
glucose and galactose.
Pesticides: Most commercialy used
chemical pesticides and fertilizers have
proved hazardous beyond a certain
threshold level. These chemicals,when
degraded by microorganisms or ultra
violet light,release pollutants in the
environment. Biotechnological tool can
help in such situations.
Weed control:New herbicides have
been developed,which will be selective
to the target and harmless for the non
target organisms. Genetically
engineered insert resistant plants have
also been successfully developed in
certain crop species,thus suggesting the
restricted use of pesticides in future.
Bio diversity and conservation:Human
activity has also proved devastating for
the diversity of speceis,and human
induced extinction of speceis has been
increasing at exponential rates. The
need for expanding population with an
unequal distribution of wealth has
invariably resulted in unstainable and
exploitative use of existing resources.
One of the major concerns today is the
preservation of our existing flaura and
fauna.
Biotechnological applictions have
opened up new and improved methods
of preserving plant and animal genetic
resources,and have accelerated the
evaluation of germ plasm collection for
specific traits. Maintaince of a wide
genetic base,which is an important
element of biodiversity,it essential to
the future of biotechnology and the
sustainable use of biological resources.
New technologies may increase the
value of world biodiversity if they allow
increased use of the genetic diversity of
both wild and domesticated species.
Conclusion: Biotechnology deals with
large scale production and marketing of
products and processes using live
organisms,cells or enzymes.
Biotechnology gives response to a
chemical that helps to measure the
level of damage caused for the
exposure of the toxic or the pollution
effect caused. Microbes break down
many chemicals in the environment.
Sewage treatment plants harmess
these microbial recyclers to clean up
waste water before it returns to
stream,lakes and ground water.
Reference:
 Plus two SCERT text book.
 Biotechnological applications.

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Arya

  • 2. INTRODUCTION Biotechnoligy deals with tecnioques of using live organisms or enzymes from organisms to produce products and process useful to humans. Biotecnology,the 20th centuryoff-shoot of modern biology,changed our daily life as its products brought qualitative improvement in health and food products. The application of biotechnology has helped in the environmental managementof hazardous contaminants by bioremediation. Bioremediation involves the use of naturally existing microorganisms to speed up the breaking down of biological substances and degradation of various material.
  • 3. USE OF BIOTECHNOLOGY Some of areas where biotechnology was proved very effective in environmental clean- up include; Landfill Technologies;Solid wastes account for an increasing proportion of the waste generated by carbon societies. While a part of this volume consist of a glass,plastics and other non-biodegradable material aconsiderable proportion of this made of decompsible solid organic material like food wastes from large poultery and pig farm.In large non-urbanized communities ,a common method for disposing off such biodegradble waste is the low-cost Anaerobic landfill technology . In this process ‘the solid waste are deposited in low-lying,low valve sites.
  • 4. COMPOSTING;Composting is an anaerobic microbially driven process that converts organic wastes into stable sanitary humus like material .This material can than be safely returned to the natural environment . This method is actually a low moisture ,solid substrate fermentation process. The primary aim of a composting operation is to obtain final compost with a desired product quality in a limited time period and within limited compost. The basic biological reaction of the composting process is the oxidation of mixed organic substrates to products CO2 and water and other organic by-products. It is important to ensure that a composting plant function under environmently,safe conditions. Bioremediation;Various products generated by the modern technoiogies are posing a great threat tothe natural breakdown process and the natural mechanisms to maintaining
  • 5. ecological balance . Many of the pollutants are complex in nature and arehence difficult to breakdown. Such pollutants are accumulating in natural environment to an alarming rate. The application of biotechnology has heiped in the environmental management of such hazardous control by bioremediation.This process is also reffered as bio-treatment. Bioremediation involves the use of naturally existing microorganisms to speed up the breaking down of biological substances and degradation of various material. BIOSENSORS;Biosensors are biophysical devices that can detect and measures the quantities of specific subject in vareity of environments. Biosensors include enzymes’antibodies and even microorganisms and these can be used for
  • 6. clinical,immunological,genetics and other research purposes. The biosensors probes are used to detect and monitor pollutants in the environment. These biosensors are non destructive in nature ,and can utilise whole cell or a specific molecules like enzymes as bioremetic detection. Their other advantages include rapid analysis,specificity and accurate reprodutibility. OIL EATING BUGS;Accidental oil spills pose great threat to ocean environments .such spills have a direct impact marine organisms. To encounter his problem, scientists have know develpoed livingorganisms to clean up the oil spills.
  • 7. The most common oil eating microorganisms are bacteria and fungi. Pencillium species has also been found to possess degrading features,but its effect needs much more than the genetically engeenered bacterium. Many other microorganisms like the Alcanivorax bacteria are also capable of degrading petroleum products. Designer Bugs:More than hundred thousand different chemical compounds are produced in the world every year. While some of these chemicals are degradable,others like chlorinated compounds are resistant to microbial degradation.
  • 8. Biomining:Among the oldest industries in the world,mining is the source of alarming level of environmental pollution. Modern biotechnology is now being used to improve the environment surronding mining area through various microorganisms. A bactirium Thiobacillus ferroxidans has been used to back out copper from mine tailings. This bactirium is naturally present in certain sulphur containing materials,and can be used to oxidise inorganic compounds like copper sulfide minarals. This process releases acids and oxidising solutions of ferric irons that can wash out metals from the crude ore. These bacteria chew up the ore and
  • 9. release copper that can subsequently be collected. Pollution control: With the help of modern biotechnology,naturally occuring biocatalysts can be used to detoxify harmful chemicals being released into the environment such biocatalysts have helped get rid of carcinogenic compounds like methylene chloride from industrial waste. These speacial bacteria are exposed to the waste in a bioreactor,where in the bacteria consume the noxious chemical and convert it into water,co2 and salts,thus completly destroying the chemical compound. A species of bacteria Geobacter metallireduces is also reffered to remove uranium from drainage water in mining
  • 10. operations,and from contaminated ground waters. The isolation and subsequent characterisation of various important genes will help in developing strains that can degrade a wide range of pollutants. Using molecular manipulations can also help tailor bacteria to use them for removing specific toxicants. Treating industrial wastes:Wastes from the paper and pulp industries contain high levels of cellulose and ligno cellulose,which pose massive treatment problems. Cellulose is extremely resistant to enzyme breakdown,and becomes resistant to both chemical and enzymatic attack when bound to lignin. Since lignin’s and
  • 11. carbohydrates are interlinked in woods,it becomes difficult to delignify the pulp. Disposal is now being handheld by various biotechnology approaches. These include: 1) Treating whey with proper strains of microbers and nutrients. 2) Direct fermentation of lactose to ethanol. 3) Hydrolysis of lactose to glucose and galactose. Pesticides: Most commercialy used chemical pesticides and fertilizers have proved hazardous beyond a certain threshold level. These chemicals,when degraded by microorganisms or ultra violet light,release pollutants in the
  • 12. environment. Biotechnological tool can help in such situations. Weed control:New herbicides have been developed,which will be selective to the target and harmless for the non target organisms. Genetically engineered insert resistant plants have also been successfully developed in certain crop species,thus suggesting the restricted use of pesticides in future. Bio diversity and conservation:Human activity has also proved devastating for the diversity of speceis,and human induced extinction of speceis has been increasing at exponential rates. The need for expanding population with an unequal distribution of wealth has
  • 13. invariably resulted in unstainable and exploitative use of existing resources. One of the major concerns today is the preservation of our existing flaura and fauna. Biotechnological applictions have opened up new and improved methods of preserving plant and animal genetic resources,and have accelerated the evaluation of germ plasm collection for specific traits. Maintaince of a wide genetic base,which is an important element of biodiversity,it essential to the future of biotechnology and the sustainable use of biological resources. New technologies may increase the value of world biodiversity if they allow
  • 14. increased use of the genetic diversity of both wild and domesticated species. Conclusion: Biotechnology deals with large scale production and marketing of products and processes using live organisms,cells or enzymes. Biotechnology gives response to a chemical that helps to measure the level of damage caused for the exposure of the toxic or the pollution effect caused. Microbes break down many chemicals in the environment. Sewage treatment plants harmess these microbial recyclers to clean up waste water before it returns to stream,lakes and ground water.
  • 15. Reference:  Plus two SCERT text book.  Biotechnological applications.