7. Figure 17.2
Minimal medium
No growth:
Mutant cells
cannot grow
and divide
Growth:
Wild-type
cells growing
and dividing
EXPERIMENT RESULTS
CONCLUSION
Classes of Neurospora crassa
Wild type Class I mutants Class II mutants Class III mutants
Minimal
medium
(MM)
(control)
MM +
ornithine
MM +
citrulline
Condition
MM +
arginine
(control)
Summary
of results
Can grow with
or without any
supplements
Can grow on
ornithine,
citrulline, or
arginine
Can grow only
on citrulline or
arginine
Require arginine
to grow
Wild type
Class I mutants
(mutation in
gene A)
Class II mutants
(mutation in
gene B)
Class III mutants
(mutation in
gene C)
Gene
(codes for
enzyme)
Gene A
Gene B
Gene C
Precursor Precursor Precursor Precursor
Enzyme A Enzyme A Enzyme A Enzyme A
Enzyme B Enzyme B Enzyme B Enzyme B
Enzyme C Enzyme C Enzyme C Enzyme C
Ornithine Ornithine Ornithine Ornithine
Citrulline Citrulline Citrulline Citrulline
Arginine Arginine Arginine Arginine
8. Figure 17.2a
Minimal medium
No growth:
Mutant cells
cannot grow
and divide
Growth:
Wild-type
cells growing
and dividing
EXPERIMENT
9. Figure 17.2b
RESULTS
Classes of Neurospora crassa
Wild type Class I mutants Class II mutants Class III mutants
Minimal
medium
(MM)
(control)
MM +
ornithine
MM +
citrulline
Condition
MM +
arginine
(control)
Summary
of results
Can grow with
or without any
supplements
Can grow on
ornithine,
citrulline, or
arginine
Can grow only
on citrulline or
arginine
Require arginine
to grow
Growth
No
growth
10. Figure 17.2c
CONCLUSION
Wild type
Class I mutants
(mutation in
gene A)
Class II mutants
(mutation in
gene B)
Class III mutants
(mutation in
gene C)
Gene
(codes for
enzyme)
Gene A
Gene B
Gene C
Precursor Precursor Precursor Precursor
Enzyme A Enzyme A Enzyme A Enzyme A
Enzyme B Enzyme B Enzyme B Enzyme B
Ornithine Ornithine Ornithine Ornithine
Enzyme C Enzyme C Enzyme CEnzyme C
Citrulline Citrulline Citrulline Citrulline
Arginine Arginine Arginine Arginine
28. Figure 17.5
Second mRNA base
FirstmRNAbase(5′endofcodon)
ThirdmRNAbase(3′endofcodon)
UUU
UUC
UUA
CUU
CUC
CUA
CUG
Phe
Leu
Leu
Ile
UCU
UCC
UCA
UCG
Ser
CCU
CCC
CCA
CCG
UAU
UAC
Tyr
Pro
Thr
UAA Stop
UAG Stop
UGA Stop
UGU
UGC
Cys
UGG Trp
GC
U
U
C
A
U
U
C
C
C
A
U
A
A
A
G
G
His
Gln
Asn
Lys
Asp
CAU CGU
CAC
CAA
CAG
CGC
CGA
CGG
G
AUU
AUC
AUA
ACU
ACC
ACA
AAU
AAC
AAA
AGU
AGC
AGA
Arg
Ser
Arg
Gly
ACGAUG AAG AGG
GUU
GUC
GUA
GUG
GCU
GCC
GCA
GCG
GAU
GAC
GAA
GAG
Val Ala
GGU
GGC
GGA
GGG
Glu
Gly
G
U
C
A
Met or
start
UUG
G
37. Figure 17.7-2 Promoter
RNA polymerase
Start point
DNA
5′
3′
Transcription unit
3′
5′
Initiation
5′
3′
3′
5′
Nontemplate strand of DNA
Template strand of DNA
RNA
transcript
Unwound
DNA
1
38. Figure 17.7-3 Promoter
RNA polymerase
Start point
DNA
5′
3′
Transcription unit
3′
5′
Elongation
5′
3′
3′
5′
Nontemplate strand of DNA
Template strand of DNA
RNA
transcript
Unwound
DNA
2
3′
5′3′
5′
3′
Rewound
DNA
RNA
transcript
5′
Initiation1
39. Figure 17.7-4 Promoter
RNA polymerase
Start point
DNA
5′
3′
Transcription unit
3′
5′
Elongation
5′
3′
3′
5′
Nontemplate strand of DNA
Template strand of DNA
RNA
transcript
Unwound
DNA
2
3′
5′3′
5′
3′
Rewound
DNA
RNA
transcript
5′
Termination3
3′
5′
5′
Completed RNA transcript
Direction of transcription (“downstream”)
5′
3′
3′
Initiation1
42. Figure 17.8
Transcription initiation
complex forms
3
DNA
Promoter
Nontemplate strand
5′
3′
5′
3′
5′
3′
Transcription
factors
RNA polymerase II
Transcription factors
5′
3′
5′
3′
5′
3′
RNA transcript
Transcription initiation complex
5′
3′
TATA box
T
T T T T T
A AA AA
A A
T
Several transcription
factors bind to DNA
2
A eukaryotic promoter1
Start point Template strand
44. Nontemplate
strand of DNA
RNA nucleotides
RNA
polymerase
Template
strand of DNA
3′
3′5′
5′
5′
3′
Newly made
RNA
Direction of transcription
A
A A A
A
A
A
T
T
T
T
TTT G
G
G
C
C C
C
C
G
C CC A A
A
U
U
U
end
Figure 17.9
67. (b) Three-dimensional structure
(c) Symbol used
Anticodon Anticodon
3′ 5′
Hydrogen
bonds
Amino acid
attachment
site5′
3′
in this book
A A G
Figure 17.15b
73. Aminoacyl-tRNA
synthetase (enzyme)
Amino acid
P P P Adenosine
ATP
P
P
P
P
Pi
i
i
Adenosine
tRNA
AdenosineP
tRNA
AMP
Computer model
Amino
acid
Aminoacyl-tRNA
synthetase
Aminoacyl tRNA
(“charged tRNA”)
Figure 17.16-4
75. tRNA
molecules
Growing
polypeptide Exit tunnel
E P
A
Large
subunit
Small
subunit
mRNA
5′
3′
(a) Computer model of functioning ribosome
Exit tunnel Amino end
A site (Aminoacyl-
tRNA binding site)
Small
subunit
Large
subunit
E P A
mRNA
E
P site (Peptidyl-tRNA
binding site)
mRNA
binding site
(b) Schematic model showing binding sites
E site
(Exit site)
(c) Schematic model with mRNA and tRNA
5′ Codons
3′
tRNA
Growing polypeptide
Next amino
acid to be
added to
polypeptide
chain
Figure 17.17
77. Figure 17.17b
Exit tunnel
A site (Aminoacyl-
tRNA binding site)
Small
subunit
Large
subunit
P A
P site (Peptidyl-tRNA
binding site)
mRNA
binding site
(b) Schematic model showing binding sites
E site
(Exit site)
E
78. Figure 17.17c
Amino end
mRNA
E
(c) Schematic model with mRNA and tRNA
5′ Codons
3′
tRNA
Growing polypeptide
Next amino
acid to be
added to
polypeptide
chain
102. Figure 17.23
Wild-type hemoglobin
Wild-type hemoglobin DNA
3′
3′
3′5′
5′ 3′
3′5′
5′
5′5′3′
mRNA
A AG
C T T
A AG
mRNA
Normal hemoglobin
Glu
Sickle-cell hemoglobin
Val
A
A
AUG
G
T
T
Sickle-cell hemoglobin
Mutant hemoglobin DNA
C
105. Wild type
DNA template strand
mRNA5′
5′
3′
Protein
Amino end
A instead of G
(a) Nucleotide-pair substitution
3′
3′
5′
Met Lys Phe Gly Stop
Carboxyl end
T T T T T
TTTTTA A A A A
AAAACC
C
C
A
A A A A A
G G G G
GC C
G GGU U U U UG
(b) Nucleotide-pair insertion or deletion
Extra A
3′
5′
5′
3′
Extra U
5′ 3′
T T T T
T T T T
A
A A A
A
AT G G G G
GAAA
AC
CCCC A
T3′5′
5′ 3′
5′T T T T TAAAACCA AC C
TTTTTA A A A ATG G G G
U instead of C
Stop
UA A A A AG GGU U U U UG
MetLys Phe Gly
Silent (no effect on amino acid sequence)
T instead of C
T T T T TAAAACCA GT C
T A T T TAAAACCA GC C
A instead of G
CA A A A AG AGU U U U UG UA A A AG GGU U U G AC
AA U U A AU UGU G G C UA
GA U A U AA UGU G U U CG
Met Lys Phe Ser
Stop
Stop Met Lys
missing
missing
Frameshift causing immediate nonsense
(1 nucleotide-pair insertion)
Frameshift causing extensive missense
(1 nucleotide-pair deletion)
missing
T T T T TTCAACCA AC G
AGTTTA A A A ATG G G C
Leu Ala
Missense
A instead of T
TTTTTA A A A ACG G A G
A
CA U A A AG GGU U U U UG
TTTTTA T A A ACG G G G
Met
Nonsense
Stop
U instead of A
3′
5′
3′5′
5′
3′
3′
5′
5′
3′
3′5′ 3′
Met Phe Gly
No frameshift, but one amino acid missing
(3 nucleotide-pair deletion)
missing
3′
5′
5′
3′
5′ 3′
U
T CA AA CA TTAC G
TA G T T T G G A ATC
T T C
A A G
Met
3′
T
A
Stop
3′
5′
5′
3′
5′ 3′
Figure 17.24
106. Figure 17.24a
Wild type
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
A instead of G
(a) Nucleotide-pair substitution: silent
StopMet Lys Phe Gly
U instead of C
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
5′
3′
3′
5′A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
G G G G
A
A
A G A A A AG GGU U U U U
T
U 3′5′
107. Figure 17.24b
Wild type
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
T instead of C
(a) Nucleotide-pair substitution: missense
StopMet Lys Phe Ser
A instead of G
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
5′
3′
3′
5′A
A A
A A A A
A AT
T T T T T
T T TT
C C T C
G
G
GA
A G A A A AA GGU U U U U 3′5′
A C
C
G
108. Figure 17.24c
Wild type
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
A instead of T
(a) Nucleotide-pair substitution: nonsense
Met
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
5′
3′
3′
5′A
A
A A A A
A AT
T A T T T
T T TT
C C C
G
G
GA
A G U A A AGGU U U U U 3′5′
C
C
G
T instead of C
C
GT
U instead of A
G
Stop
110. Figure 17.24d
Wild type
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
(b) Nucleotide-pair insertion or deletion: frameshift causing
immediate nonsense
Extra A
Extra U
5′
3′
5′
3′
3′
5′
Met
1 nucleotide-pair insertion
Stop
A C A A GT T A TC T A C G
T A T AT G T CT GG A T GA
A G U A U AU GAU G U U C
A T
A
AG
111. Figure 17.24e
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
(b) Nucleotide-pair insertion or deletion: frameshift causing
extensive missense
Wild type
missing
missing
A
U
A A AT T TC C A T TC C G
A AT T TG GA A ATCG G
A G A A GU U U C A AG G U 3′
5′
3′
3′
5′
Met Lys Leu Ala
1 nucleotide-pair deletion
5′
112. Figure 17.24f
DNA template strand
mRNA5′
5′
Protein
Amino end
Stop
Carboxyl end
3′
3′
3′
5′
Met Lys Phe Gly
A
A
A A
A A A A
A AT
T T T T T
T T TT
C C C C
C
C
G G G G
G
G
A
A A A AG GGU U U U U
(b) Nucleotide-pair insertion or deletion: no frameshift, but one
amino acid missing
Wild type
AT C A A A A T TC C G
T T C missing
missing
Stop
5′
3′
3′
5′
3′5′
Met Phe Gly
3 nucleotide-pair deletion
A GU C A AG GU U U U
T GA A AT T TT CG G
A A G
124. Figure 17.UN05
Type of RNA Functions
Messenger RNA (mRNA)
Transfer RNA (tRNA)
Primary transcript
Small nuclear RNA (snRNA)
Plays catalytic (ribozyme) roles and
structural roles in ribosomes