The role of S100 proteins in skin biology and physiology.ppt

1. S100 Proteins and Their Role in Some Dermatological Diseases By M.Y.ABDEL-MAWLA,MD ZAGAZIG FACULTY OF MEDICINE,EGYPT

2. S100 Proteins Structure and Functions

15. Structure of Proflaggrin

18. Calcium and S100 proteins

19. S100 Proteins Functions

21. S100 Proteins in Epidermis

22. S100 proteins in epidermis

25. Protein targets of S100 proteins

26. S100 Proteins and Their Role in Some Skin Diseases

29. Schematic summary of important innate immunity events in the pathophysiology of psoriasis

31. S100 Proteins and Tumors

33. S100 proteins and possible cancer diseases

35. S100 Protein immune staging in melanocytic melanoma Biomarker Common nevi Spitz nevus Melanoma S100A Protein 56% weak dermal 100% strong, diffuse 33% weak dermal

36. S100 Protein immune staging in melanocytic nevi and their tumor mimics Marker Sensitivity/ specificity melanoma Carcinoma Sarcoma/soft tissue tumors Epithelioid cells Spindle cells S100 Proteins < 95%/ 75%-87% + + (98% Sensitivity) -(+Myoepithelial Carcinoma) +/-

37. Staining with S100 protein and other marker of neural hyperplasias and neoplasms Neural proliferation S100 Neurofilament Epithelial membrane antigen (EMA ) Other Traumatic neuroma + + + Neuron specific enolase positive Palisaded encapsulated neuroma + + + For capsule Schwannoma + - + for capsule only Vimentin positive Neurofibroma + Variable - Plexiform neurofibroma + + Neurothekeoma + - Variable Vimentin variable;usually EMA negative Cellular Neurothekeoma - - CD57 positive;Ki-Mip positive perineurioma - - + Vimentin positive;neuron-specific enolase negative Malignant peripheral nerve sheath tumor + + Variable Vimentin variable;cytokeratin negative ;neuron-specific enolase positive Primitive neuroectodermal tumor + If differentiated Neuron-specific enolase positive;CD99 positive Granular cell tumor + Neuron-specific enolase positive + = Positive: - = negative.

38. How to detect S100 Proteins?

40. Summary and Conclusion

42. Thank you