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Sample Size 
The indispensable A/B test calculation 
that you’re not making.
As Marketers, 
many of us run A/B Tests
We test copy
We test design
We test subject lines
We choose winners
Version A is converting better than 
Version B and statistical significance 
has breached 95%. 
So, Version A won.
Version A is converting better than 
Version B and statistical significance 
has breached 95%. 
So, Version A won. 
OR DID IT?
That math is half-baked
Suppose you check an A/B Test twice: 
Once after 200 impressions and then after 500. 
Then you end the test.
Now, instead, suppose you stop the test once 
you reach significance:
Now, suppose you stop the experiment as soon 
as there is a significant result: 
FALSE POSITIVE!
How often will you get 
a false positive?
Assuming you check results after every impression and 
stop once you reach significance…. 
26.1% 
So you just went from 95% confidence to 74% 
This is a worst-case scenario. BUT, some test platforms do this automatically!
OK…well, then when should I stop an 
A/B test?
SAMPLE SIZE 
Dictates how long to run a test
SAMPLE SIZE 
• Used religiously in the pharmaceutical Industry, 
economic studies, etc…
https://www.optimizely.com/resources/sample-size-calculator
Agenda 
1. How we put this into practice on a website test 
2. How we applied these learnings to email testing: 
• Open rates 
• Click to Open Rates 
• Conversion Rates
A/B Testing on your website 
Here’s your new test process: 
1. Determine your baseline conversion rate (or click rate, or 
download rate, etc..) 
2. Decide how long you are willing to wait for a result. Convert your 
unique traffic metric to a sample size. 
3. Adjust MDE (Minimum Detectable Effect) until your Sample Size is 
just under the target you determined in #2 above. 
4. Re-adjust MDE until you are content. 
5. Start the test, and don’t stop until you hit the sample size.
Case Study: Item Urgency
Case Study: Item Urgency 
TEST (VERSION A): 
INVENTORY NOTIFICATION 
CONTROL (VERSION B): 
NO INVENTORY NOTIFICATION
STEP 1 – We determined our baseline conversion rate
STEP 2 – Calculate Target Sample Size 
We initially decided we wanted a result in 2 weeks. 
So we took the last 2 weeks of unique product page views:
STEP 2 – Calculate Target Sample Size 
We then divided that number by two (since we’ll have two test 
segments) 
Divided by two again to account for desktop traffic only 
Then multiplied by 5% (since the message only displays on 5% of 
product pages) 
Sample Size -> 12,351
This gave us 30% MDE (Conversion Lift). This is unrealistic
How about 10% ?
107,105 unique visits ~ 17 weeks
Wow, that’s a long time…
Yep.
You’re probably not 
running your tests long 
enough
WAIT A MINUTE. 
MY A/B TEST PLATFORM SAYS NOTHING 
ABOUT SAMPLE SIZE…
EVERYONE WANTS INSTANT GRATIFICATION
YOUR A/B TEST PLATFORM IS HAPPY TO SELL IT
Quietly assuming you have calculated 
sample size on your own
Item Urgency - Test Results 
We are over 4 weeks in…. 
*Conv. rate is higher than expected because test platform runs on 7 day conversion window.
Item Urgency - Test Results 
Lift is over 10% 
Note the spike in the beginning and the increased stabilization with time
Test Results 
The effect is slowly approaching the MDE
Test Results 
Significance is now over 95%, but it’s been up and down. 
Many marketers would stop the test on 9/5 and declare a 57% Lift.
Email Testing
After learning about Sample Size, 
we reconsidered our email testing strategy 
• Open Rate (Subject line testing) 
• Click-to-Open (CTO) Rate 
• Conversion Rate
OPEN RATE 
We used sample size to gut check the 
size of our subject line test segments
OPEN RATE 
Remember, for the sample size calculator, 
you need the baseline conversion rate and 
then the sample size, and that will give you 
MDE.
OPEN RATE 
First, we needed the baseline conversion open rate
OPEN RATE 
Our open rates typically end up ~ 17% , but when 
we make the call on our winning subject line, open 
rates are usually around 7%.
OPEN RATE 
Next we need the sample size
OPEN RATE 
We always test 4 different subject lines. 
We had been sending each subject line to 10,000 
customers. 
So, sample size ~ 10,000
OPEN RATE 
Plugging these numbers in, this would only detect 13% open rate lift or higher
OPEN RATE 
13% lift on 17% open rate is 19.2%. 
We rarely see subject lines this high 
We needed a lower MDE to make sure we could detect 
more winners…
OPEN RATE 
We ended up doubling our subject line segment to 
80,000, giving us an MDE ~ 9.2%
CTO 
First we needed the baseline
CTO 
We averaged the last 10 weeks -> 11% CTO
CTO 
Sample size = ½ of the avg opens count
CTO 
We averaged the last 10 weeks -> Avg 
opens = 107,000 / 2 = 53,500
CTO
CTO 
4.4% CTO lift is a very reasonable goal for a test. 
This showed us that we could trust most of the 
results of our past CTO tests.
GRID vs. FREE FORM 
15.7% CTO Lift
PRODUCT NAMES vs. NO PRODUCT NAMES 
22.6% CTO Lift
Conversion Rate 
We had been making many email decisions 
after reaching significance 
on a conversion rate lift
Conversion Rate 
Time for a reality check.
Conversion Rate 
Baseline Conversion Rate ~ 1.5%
Conversion Rate 
Sample Size = ½ Average # Clicks -> 6,000
Conversion Rate
Conversion Rate 
38% is ASTRONOMICAL
Conversion Rate 
To get meaningful results for conversion rate, 
consider running an email test many times, so that 
you can eventually reach the necessary sample 
size.
Takeaways 
This is the MDE curve again. Remember what this looks like. 
The longer you run a test, the lower MDE will be. 
The more traffic volume you have, the faster MDE will drop
Takeaways 
For Web Testing 
• If you stop your A/B tests once you reach statistical significance, you are increasing your chances of finding 
false positives 
• Calculating sample size will give you a clear stop date and an MDE 
• MDE and sample size are inversely related – The lower the MDE, the larger the sample size 
• Most likely, your A/B tests need to run much longer than you realize 
For Email Testing 
• Use sample size to determine the size of your subject line test segments 
• Your CTO tests are probably reaching the necessary sample size 
• Your Conversion tests are probably not hitting sample size
Sources 
Kyle Rush – Mozcon 2014 Presentation 
https://seomoz.box.com/shared/static/2fw6yevkkmmdum 
z431j4.pdf 
Evan Miller – How not to run an AB test 
http://www.evanmiller.org/how-not-to-run-an-ab-test. 
html
Zack Notes 
Digital Marketing 
Manager 
zack@uncommongoods.com 
@zacknotes 
slideshare.net/zacknotes1/presentations
Appendix
GRID vs. FREE FORM
PRODUCT NAMES vs. NO PRODUCT NAMES
What do you do if a test reaches sample size 
and your lift < MDE?
You can either extend the test and accept a 
lower MDE or Move On.

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SAMPLE SIZE – The indispensable A/B test calculation that you’re not making

  • 1. Sample Size The indispensable A/B test calculation that you’re not making.
  • 2. As Marketers, many of us run A/B Tests
  • 7. Version A is converting better than Version B and statistical significance has breached 95%. So, Version A won.
  • 8. Version A is converting better than Version B and statistical significance has breached 95%. So, Version A won. OR DID IT?
  • 9. That math is half-baked
  • 10. Suppose you check an A/B Test twice: Once after 200 impressions and then after 500. Then you end the test.
  • 11. Now, instead, suppose you stop the test once you reach significance:
  • 12. Now, suppose you stop the experiment as soon as there is a significant result: FALSE POSITIVE!
  • 13.
  • 14. How often will you get a false positive?
  • 15. Assuming you check results after every impression and stop once you reach significance…. 26.1% So you just went from 95% confidence to 74% This is a worst-case scenario. BUT, some test platforms do this automatically!
  • 16. OK…well, then when should I stop an A/B test?
  • 17. SAMPLE SIZE Dictates how long to run a test
  • 18. SAMPLE SIZE • Used religiously in the pharmaceutical Industry, economic studies, etc…
  • 20. Agenda 1. How we put this into practice on a website test 2. How we applied these learnings to email testing: • Open rates • Click to Open Rates • Conversion Rates
  • 21. A/B Testing on your website Here’s your new test process: 1. Determine your baseline conversion rate (or click rate, or download rate, etc..) 2. Decide how long you are willing to wait for a result. Convert your unique traffic metric to a sample size. 3. Adjust MDE (Minimum Detectable Effect) until your Sample Size is just under the target you determined in #2 above. 4. Re-adjust MDE until you are content. 5. Start the test, and don’t stop until you hit the sample size.
  • 22. Case Study: Item Urgency
  • 23. Case Study: Item Urgency TEST (VERSION A): INVENTORY NOTIFICATION CONTROL (VERSION B): NO INVENTORY NOTIFICATION
  • 24. STEP 1 – We determined our baseline conversion rate
  • 25. STEP 2 – Calculate Target Sample Size We initially decided we wanted a result in 2 weeks. So we took the last 2 weeks of unique product page views:
  • 26. STEP 2 – Calculate Target Sample Size We then divided that number by two (since we’ll have two test segments) Divided by two again to account for desktop traffic only Then multiplied by 5% (since the message only displays on 5% of product pages) Sample Size -> 12,351
  • 27. This gave us 30% MDE (Conversion Lift). This is unrealistic
  • 29. 107,105 unique visits ~ 17 weeks
  • 30. Wow, that’s a long time…
  • 31. Yep.
  • 32. You’re probably not running your tests long enough
  • 33. WAIT A MINUTE. MY A/B TEST PLATFORM SAYS NOTHING ABOUT SAMPLE SIZE…
  • 34. EVERYONE WANTS INSTANT GRATIFICATION
  • 35. YOUR A/B TEST PLATFORM IS HAPPY TO SELL IT
  • 36. Quietly assuming you have calculated sample size on your own
  • 37. Item Urgency - Test Results We are over 4 weeks in…. *Conv. rate is higher than expected because test platform runs on 7 day conversion window.
  • 38. Item Urgency - Test Results Lift is over 10% Note the spike in the beginning and the increased stabilization with time
  • 39. Test Results The effect is slowly approaching the MDE
  • 40. Test Results Significance is now over 95%, but it’s been up and down. Many marketers would stop the test on 9/5 and declare a 57% Lift.
  • 42. After learning about Sample Size, we reconsidered our email testing strategy • Open Rate (Subject line testing) • Click-to-Open (CTO) Rate • Conversion Rate
  • 43. OPEN RATE We used sample size to gut check the size of our subject line test segments
  • 44. OPEN RATE Remember, for the sample size calculator, you need the baseline conversion rate and then the sample size, and that will give you MDE.
  • 45. OPEN RATE First, we needed the baseline conversion open rate
  • 46. OPEN RATE Our open rates typically end up ~ 17% , but when we make the call on our winning subject line, open rates are usually around 7%.
  • 47. OPEN RATE Next we need the sample size
  • 48. OPEN RATE We always test 4 different subject lines. We had been sending each subject line to 10,000 customers. So, sample size ~ 10,000
  • 49. OPEN RATE Plugging these numbers in, this would only detect 13% open rate lift or higher
  • 50. OPEN RATE 13% lift on 17% open rate is 19.2%. We rarely see subject lines this high We needed a lower MDE to make sure we could detect more winners…
  • 51. OPEN RATE We ended up doubling our subject line segment to 80,000, giving us an MDE ~ 9.2%
  • 52. CTO First we needed the baseline
  • 53. CTO We averaged the last 10 weeks -> 11% CTO
  • 54. CTO Sample size = ½ of the avg opens count
  • 55. CTO We averaged the last 10 weeks -> Avg opens = 107,000 / 2 = 53,500
  • 56. CTO
  • 57. CTO 4.4% CTO lift is a very reasonable goal for a test. This showed us that we could trust most of the results of our past CTO tests.
  • 58. GRID vs. FREE FORM 15.7% CTO Lift
  • 59. PRODUCT NAMES vs. NO PRODUCT NAMES 22.6% CTO Lift
  • 60. Conversion Rate We had been making many email decisions after reaching significance on a conversion rate lift
  • 61. Conversion Rate Time for a reality check.
  • 62. Conversion Rate Baseline Conversion Rate ~ 1.5%
  • 63. Conversion Rate Sample Size = ½ Average # Clicks -> 6,000
  • 65. Conversion Rate 38% is ASTRONOMICAL
  • 66. Conversion Rate To get meaningful results for conversion rate, consider running an email test many times, so that you can eventually reach the necessary sample size.
  • 67. Takeaways This is the MDE curve again. Remember what this looks like. The longer you run a test, the lower MDE will be. The more traffic volume you have, the faster MDE will drop
  • 68. Takeaways For Web Testing • If you stop your A/B tests once you reach statistical significance, you are increasing your chances of finding false positives • Calculating sample size will give you a clear stop date and an MDE • MDE and sample size are inversely related – The lower the MDE, the larger the sample size • Most likely, your A/B tests need to run much longer than you realize For Email Testing • Use sample size to determine the size of your subject line test segments • Your CTO tests are probably reaching the necessary sample size • Your Conversion tests are probably not hitting sample size
  • 69. Sources Kyle Rush – Mozcon 2014 Presentation https://seomoz.box.com/shared/static/2fw6yevkkmmdum z431j4.pdf Evan Miller – How not to run an AB test http://www.evanmiller.org/how-not-to-run-an-ab-test. html
  • 70. Zack Notes Digital Marketing Manager zack@uncommongoods.com @zacknotes slideshare.net/zacknotes1/presentations
  • 73. PRODUCT NAMES vs. NO PRODUCT NAMES
  • 74. What do you do if a test reaches sample size and your lift < MDE?
  • 75. You can either extend the test and accept a lower MDE or Move On.

Notes de l'éditeur

  1. I have a thought experiment for you…
  2. All of the scenarios have the same end result, except for scenario #3
  3. Note the baseline, minimum detectable effect and sample size Baseline = preexisting conversion rate, click rate, open rate, etc. Minimum Detectable Effect or MDE = the minimum lift you will be able to detect once you’ve reached the sample size. So here’s the gist of this presentation – If you are running a test on a page with a baseline conversion rate of 3% - and you run the test until your test segment reaches 10,316 impressions. If you’re observed conversion lift is below 20%, you can’t declare a winner. Even if you’ve reached statistical significance. You either need to keep your test running or move on.
  4. This is your step by step guide to sample size testing. I’m going to go over it very briefly. This slide is more of a resource for you to come back to when you set up your test.
  5. We’re currently running this test on our product pages
  6. This is a message that fires when there are less than 5 items in inventory. You can see the test on top and the control below.
  7. We put 1.16% in conversion rate. Then we turn the MDE up until our sample size is just under 12,351
  8. Nobody wants to wait 17 weeks for a test result, but if you make a call too early, you could be shooting yourself in the foot, buying a false positive, and deploying a new design which is actually making your site worse
  9. BACK TO ITEM URGENCY This is a chart of conversion rate. Test in Blue. Control in Red
  10. Note how the MDE contains the lift with an upper bound Also note how lift is approaching MDE towards the end
  11. Red line is significance Green line is the 95% mark Note that the significance crossed 95% in the beginning and then came back down, and it’s now rising above 95% again.
  12. 17% after a week but 7% after 2 hours when we make the call
  13. 17% after a week but 7% after 2 hours when we make the call
  14. Here’s a few examples of CTO tests we ran and at the end, in the appendix, I’ve included all data for you to look at afterwards
  15. I went back through all of our tests. In the 3 years since we’ve started A/B testing in emails, the only time we’ve hit sample size for conversion rate is when we’ve tested putting prices in an email (vs. leaving them out). And Unfortunately, this lift in conversion was countered by an equal drop in CTO
  16. The lift from the vast majority of your tests will never reach MDE. Be more comfortable reporting “no statistical difference”