Standardization information on Andrographis, Bacopa, Commiphora, Glycyrrhiza, Ocimum, Phyllanthus amarus, Amla, Piper longum, Coleus and Ashwagandha extracts

2. Quality Assessment of Selected Indian
Medicinal Plants
Volume 1
Amit Agarwal
Balasubramanian Murali
2010
A Joint Publication from
National Medicinal Plants Board R & D Centre
Department of AYUSH, # 5B, Veerasandra Industrial Area,
Ministry of Health & Family Welfare, 19th KM Stone, Hosur Road,
Govt. of India, New Delhi, India. Bangalore, India.
web: www.nmpb.nic.in web: www.naturalremedy.com
i

3. All rights reserved
No part of this publication may be reproduced, stored in a retrieval system or
transmitted, in any form or any means, electronic, mechanical, photocopying, recording
or otherwise, without prior written permission of National Medicinal Plants Board,
New Delhi and Natural Remedies Pvt Ltd., Bangalore, India.
© 2010 Natural Remedies Pvt Ltd
Bangalore
Price : Inland : 1000
Foreign : $ 50
Published by : National Medicinal Plants Board
&
M/s Natural Remedies Pvt Ltd.
Printed at : M/s Mangala Process
Bangalore - 560 027.
ISBN 978-81-910093-0-9
This is not a legal document
Cover page:
Phyllanthus emblica
Andrographis paniculata
Instrumentation Laboratory of Natural Remedies Pvt Ltd., Bangalore.
Disclaimer:
National Medicinal Plants Board (NMPB) neither endorses nor opposes any data contained in this book. The
information presented here is considered to be reliable; however NMPB has not independently verified this
information. The readers are encouraged to correspond directly with the authors for any clarifications /
suggestions for improvement.
Information on the tradtional uses, desirable phytochemical constituents, regulatory status, traditional uses and
other information has been compiled from published literature.
ii

4. Acknowledgement
We wish to thank the National Medicinal Plants Board (NMPB) and the management
of Natural Remedies for providing the necessary financial support for completion of
this project. Our special thanks to Shri. B.S.Sajwan, CEO, NMPB, for his constant
encouragement and practical guidance. We also wish to thank Dr. D.B.A.Narayana
for his technical inputs at various stages of this project.
We wish to thank the experts of the project screening committee of NMPB viz., Dr. H
P Singh, Dr. Rajendra Gupta, Dr. P D Sethi, Dr. C K Katiyar, Dr. Pushpangadan and
Dr. Padma Kumar for their valuable suggestions for improving the quality and utility of
this work.
The names of specific scientists from Natural Remedies R&D center who significantly
contributed to this work have been separately listed under “Members of the Technical
Team”. We wish to additionally thank the scientists from the departments of
Phytochemistry (Mr. M M Hipolith Viji, Mr. N K Bopanna), Analytical Chemistry
(Mr. B Vinod, Mr. C Prakash), Microbiology (Ms. M K Asha), ADME (Dr. Shekar
M Dethe), Cell Assay (Dr. C V Chandrasekaran), Pharmacology and Toxicology
(Dr. Krishnagouda S Goudar, Dr. V Vijayabalaji), Research Data Management (Ms.
G Sushma), Technical Documentation (Ms. T S Geetha, Mr. K Ramesh) and the
Traditional Medicine Group (Dr. K Venkateshwaralu) who have also supported this
work directly and indirectly. Without the coherent team work and support from all the
members, this work would not have been completed.
We thank the department of AYUSH, Ministry of Health and Family Welfare, Govt.
of India for providing us this opportunity.
Authors
ix

5. Members of Technical Team
Dr. M Deepak
Sr. Manager
Mr. M L Suryanarayana
Dy. Manager
Mr. M S Anand
Asst. Manager
Mr. G K Sangli
Sr. Research Officer
Mr. T Mahendran
Officer
Mr. K Sendhil Nathan
Officer
Ms.C L Prathima
Sr. Officer
Dr. P Santhan
Taxonomist
Dr. J Joshua Allan
Dy. Manager
x

6. Contents
Chapters Page No.
1. Andrographis paniculata Nees 01 - 29
(Kalmegh)
2. Bacopa monnieri (L.) Pennell 30 - 57
(Brahmi)
3. Commiphora wightii (Arn.) Bhandari 58 - 81
(Guggul)
4. Glycyrrhiza glabra L. 82 - 108
(Licorice)
5. Ocimum tenuiflorum L. 109 - 135
(Tulsi)
6. Phyllanthus amarus Schumach. 136 - 158
(Bhuiamla)
7. Phyllanthus emblica L. 159 - 180
(Amla)
8. Piper longum L. 181 - 207
(Pippali)
9. Plectranthus barbatus Andrews 208 - 228
(Coleus)
10. Withania somnifera (L.) Dunal 229 - 252
(Ashwagandha)
xi

7. Appendices
- Phytochemical reference standards A1
- About reference material A2
- Sampling A3
- Terminologies and Abbreviations A4 - A7
Annexure I
Method of analysis - Chemical A8 - A9
Annexure II
Method of analysis - Microbiological A10 - A11
Annexure III
Selection of desirable compounds A12 - A42
xii