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Timothy C. Sanchez
PO BOX 186
Vadito, NM 87579
575-779-0594
sancheztimothy77@hotmail.com
tim_san@lanl.gov
Education
Northern New Mexico College, Espanola, NM
Associate of Applied Science, Laboratory Biotechnology, May 2008
Northern New Mexico College, Espanola, NM
Bachelor of Science, Biology, May 2010
New Mexico Highlands University, Las Vegas, NM
Masters of Life Science, Natural Resource Management; December 2015
The University of New Mexico, Albuquerque, NM
Ph.D. of Engineering, Program of Study: Biomedical Engineering; Aug 2013-Present
LANL/DOE Credential
DOE Q-Clearance-Expiry: Feb 20, 2018 .
Research Experience
March - 2007-Oct 2007
ResearchExperienceUndergraduateInternship,NationalScience Foundation funded;
Northern New Mexico College-Los Alamos National Laboratory
 Evaluated mass spectrometry protein-derived data and made recommendations for
use and purchase of Protein Identificationsoftware.
 Became knowledgeable of various mass spectrometry ionization
technologies/techniques including Matrix-Assisted-Laser desorption Ionization
(MALDI),Electrospray ionization (ESI),Desorption Electrospray Ionization (DESI).
 Learned fundamental laboratory techniques including proper safeguards for sample
handling, waste generation/waste disposal, reagent storage, flammables and
corrosivehandling.
October - 2007 – May 2010
Undergraduate Research Student, Bioscience Division, Los Alamos National Laboratory
 Became proficient in Biochemistry techniques and instrumentation, i.e.
spectrophotometry, UV-VIS,multidimensional HPLC forprotein and small molecule
separations, high-affinity based separations, spectroscopy,gel electrophoresis.
 Designed and manufactured a functionalDesorption Electrospray ionization (DESI)
source to retrofit a Thermofinnigan LCQ Mass Spectrometer.
 Performed analyticalbiological assays, both quantitative/qualitative;

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 Biological sample preparation techniques including bacterial/mammalian celllysis,
protein extraction/purification, protein enzymatic digestion.
June - 2010 – 2015
Graduate Research Student- New Mexico Highlands University, Las Vegas, NM
 Understanding localclimate change effectsand interactions of fresh-water stream
loading, of limiting nutrients such as phosphorus, nitrogen. Study area: Valles
Caldera National Preserve, Jemez Springs, NM.
 Coursework included Surface hydrology,limnology, agro-ecology, watershed
management practices, Statistics, remote sensing/GIS.
 Analytical methods used: Ion-chromatography, graphite-furnace Atomic Absorption
spectrometry, and other photometric instrumentation.
 Performed extensive method development of twophotometric assays for
measurement of total phosphorus. Goals were to adapt a current EPA method to
newer instrumentation that allowed high throughput analysis, cost saving and
improved range in limit of detection. This was collaborative workbetween NMHU
and LANL.
June - 2010 – 2015
Graduate Research Student, Bioscience Division, Los Alamos National Laboratory
 Performed method development using mesoporous silica thin film as means to
analyze small molecule as a matrix-free application.
 Developed methods utilizing Ionic liquids (molten salts) used as a multifunctional
dyes extraction technique that also served as a MALDI-MS ionization matrix used for
in an FBI funded counter-terrorism project.
 Integrated experimental data using system biology softwareto understand system
level effectssuch as protein expression, protein functionand understand cellular
pathways
 Interpretation of toxological effectsof nanomaterial i.e. fullerenes, quantum dots,
exposure on skin, lung cellculture, and tissue.
 Worksupported by Laboratory Directed Research and Development-Direct
Research: Nanotoxicity:Assessing the potential health Hazards of Nanotechnology.
 Responsible forgenerating mass spectral data leading to conclusions affecting gene
and protein regulation changes as a result of nanoparticle exposure.
 Work highlighted on Los Alamos Science and Technology Magazine 1663, March
2013 publication. See http://www.lanl.gov/discover/publications/1663/2013-
march/_assets/docs/march-2013-full-print.pdf
 Defense Reduction Threat Agency funded project: ProjectATHENAcreates
surrogate human organ systems. See http://www.lanl.gov/discover/news-
release-archive/2015/June/06.15-athena-creates-surrogate-human-organ-
systems.php

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 Primary workin the ATHENAproject are:
 Provide metabolomics data: Identify small molecules that metabolize by a human-
derive organ construct being developed by our collaborative team. UPLC-IM-MS,
software analysis, and database interrogation capabilities/techniques are used.
 Responsible authority for overseeing the procurement, site preparation, installation,
validation, operation of two Ion Mobility Mass spectrometers.
 Serve as the Person-In-Charge for Mass spectrometry capability at TA-43 Bioscience
Division. Oversee, assist in experimental design, operate and carryoutall
experimental workinvolvingsample preparation, HPLC, Mass Spectrometry and
data analysis.
 Integration of UPLC-IonMobility Mass Spectrometry techniques for Metabolomics/
Proteomicsfor toxicologicalorgan screening.
 Operate a coupled Agilent Gas Chromatograph use forseparation of lipid before
employing Ion Mobility Mass spectrometry techniques.
 Proficientin Biological Safety Level 1 and 2 lab activities.
 Familiar with many analytical techniques and very proficient in troubleshooting; I
feel that is my my strong suit.
 Familiar with many softwaresuites supporting analyticalinstrumentation and learn
operation of software fairly quickly.
 Developed many method briefs, guides and standard operating procedures for
various applications.
 Managed, mentored, and oversaw all experimental activities of student researchers
in the Mass spectrometry Lab. Student ranged from high school COOP’s,
undergraduates, and graduate students. 13 students over the last 8 years.
 Very familiar with, and have standing relationships withsales and technical support
with the followinganalytical companies: Water Corporation, OI Analytics, Applied
Biosystems, AB Sciex, Dionex, Proxeon,Agilent, ThermoFisher, Bruker, and
Shimadzu.
 Collaborations: Most projects required collaboration across multiple disciplines and
throughout other divisions at LANL; other collaborators include those from
universities and industry.
 Comfortable with giving technical talks and engaging with other professionals in the
field.
Professional Publications
Biosynthesisofthe3,4-DihydroxybenzoateMoietiesofPetrobactinbyBacillus
anthracis Andrew T. Koppisch, Kinya Hotta, David T. Fox, Christy E. Ruggiero, Chu-Young
Kim, TimothySanchez,Srinivas Iyer, Cindy C. Browder, Pat J. Unkefer,Clifford J.Unkefer
TheJournal ofOrganic Chemistry 200873 (15), 5759-5765
SynthesisandCharacterizationofEthyleneGlycol SubstitutedPoly(phenylene
Vinylene)Derivatives Chun-Chih Wang, Hsinhan Tsai, Hung-Hsin Shih, Seaho Jeon, Zhihua
Xu, DarrickWilliams, Srinivas Iyer, TimothyC. Sanchez,Leeyih Wang, Mircea Cotlet, Hsing-
Lin Wang ACS AppliedMaterials&Interfaces 20102 (3),738-747

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CharacterizationofReactionIntermediateAggregatesinAnilineOxidative
PolymerizationatLowProtonConcentration ZhongfenDing, TimothySanchez,Andrea
Labouriau, Srinivas Iyer, Toti Larson, Robert Currier, Yusheng Zhao, Dali YangTheJournalof
PhysicalChemistryB 2010114 (32),10337-10346
Tetraakylphosphonium-basedionicliquidsusedinasingle-stepdye
extraction/MALDIanalysisplatformKatherine S. Lovejoy,Srinivas Iyer,Geraldine M.
Purdy,TimothyC. Sanchez,Tessui Nakagawa, Andrew T. Koppisch, RicoE. Del Sesto
AnalyticalChemistry 2011 Apr 15;83(8):2921-30
Single-PotExtraction-AnalysisofDyedWool FiberswithIonicLiquids KatherineS.
Lovejoy,Alexander J. Lou, Lauren E. Davis, TimothyC. Sanchez,Srinivas Iyer, Cynthia A.
Corley, John S. Wilkes, Russell K. Feller, David T. Fox, Andrew T. Koppisch, and Rico E. Del
Sesto AnalyticalChemistry 201284(21), 9169-9175
Using phage display selected antibodies to dissect microbiomes for complete
de novo genome sequencing of low abundance microbes Devin W. Close,
Fortunato Ferrara, Armand E. K. Dichosa, Sandeep Kumar, Ashlynn R. Daughton,
Hajnalka E. Daligault, Krista G. Reitenga, Nileena Velappan, Timothy C. Sanchez,
Srinivas Iyer, Csaba Kiss, Cliff S. Han, Andrew R. M. Bradbury BMC Microbiology
2013, 13:270
Proteomic Analysis of Detergent Resistant Membrane Domains during Early
Interaction of Macrophages with Rough and Smooth Brucella melitensis. Sabine A.
Lauer, Srinivas Iyer, Timothy Sanchez, Christian V. Forst, Brent Bowden, Kay Carlson,
Nammalwar Sriranganathan, Stephen M. Boyle PLoS ONE (2014) 9(3): e91706.
doi:10.1371/journal.pone.0091706
Novel Strategies for Biodetection: Preliminary Application to Traumatic Brain
Injury; Aaron S. Anderson, Dung M. Vu, Timothy Sanchez, Srinivas Iyer and
Harshini Mukundan (2014). MRS Proceedings, 1599, jsapmrs13-1599-7174
doi:10.1557/opl.2014.492.
Technical Talks
Omics AnalysisforToxicology
2014 Los Alamos National Laboratory Graduate Student Symposium (August, 2014)

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Postersand Presentations
SanchezT;Iyer S. Comparison of Proteomic Analysis Software. Los Alamos National
Laboratory Student Symposium, Los Alamos NM (2007)
SanchezT;Iyer S. Comparison of Proteomic Analysis Software. Arizona State
University,Tempe AZ (2007)
SanchezT;Iyer R; Gao J; Bhardwaj S; Carlson JE;Sun S; IyerS. Effectof Hexa-carboxy
Fullerenes on Differentiated Macrophages: Analysis by iTRAQ™. American Society of Mass
Spectrometry, Denver CO (2008)
SanchezT;Coronado C; Iyer R; Gao J; Bhardwaj S; Carlson JE;Sun S; Iyer S. Analysis of
iTRAQ™ ratios upon macrophage exposure to Fullerenes. Los Alamos National Laboratory
Student Symposium, Los Alamos, NM (2008)
SanchezT;Martinez JM;Martinez JA; Sanchez SM; Romero JM.Some observations on
Corrosion and Cathodic protection of Fe-Zn & Sn-Fe in Espanola, New MexicoWaters. NACE
International Conference, San Antonio, TX (2010)
SanchezT;Iyer S; Proteomic methods used to identify and quantify protein
expression induced by nanomaterials. Cinco de Mayo Science Symposium 2010;
Northern New Mexico College, Espanola NM (2010)
Lovejoy KS; Iyer S; Purdy GM; SanchezTC;Koppisch AT; Del Sesto RE.Single-Step
Identification of Dyes in Wool Fibers witha Tetraalkylphosphonium Platform.The
International Chemical Congress of Pacific Basin Societies; Honolulu, Hawaii, USA (2010)
SanchezT;Williams D; Martinez E; Use of intermittent streams to model the effectsof
Drought, predicting future consequences of mainstem streams by identifying and
assessing concentration correlations between drought low flow conditions and dissolved
solutes, New MexicoHighlands University Research Day, Las Vegas NM (2010)
SanchezT;Williams D; Martinez E; Distribution and Fate of Geothermal Solutes, EPSCOR
3rd Annual Tri-State meeting, Santa Ana Pueblo,NM (2011)
SanchezT; IyerS; IyerR; Biological effectson Human Epidermal Keratinocytes (HEK)
induced by engineered Fullerene Derivatives, Los Alamos National Laboratory Student
Symposium, Los Alamos, NM (2012)
SanchezT; Garcia L; Williams D; Medina S; Martinez E; Characterizing Variations of
PhysiochemicalParameters Influencing the Mobilization/Adsorption and Redox State of
Arsenic and Iron in Valles Caldera Streams, New Mexico Highlands University Research Day,
Las Vegas NM (2012)

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HonorsandAwards
Americorps ServiceLearning Scholarship, Summer 2000;
Phi BetaKappa honor society,GPA 4.0, University of New Mexico, Fall 2006;
DeanList, Northern MexicoCollege, Fall 2007;
HonorableMention,UGSPosterPresentation, (WAESO) Western Alliance to Expand
Student Opportunities and (MGE@MSA) More Graduate Education at Mountain States
Alliance 7th Annual Conference, Arizona State University; Summer 2007;
STEM Performance,Innovationand Retention (STEM-ASPIRe) Scholarship, Northern New
Mexico College, Fall 2008, Spring 2009, Fall 2009, Spring 2010;
National Science and Mathematics Access to Retain Talent Grant, Northern New Mexico
College, Spring 2009;
SecondPlaceAward-Cinco de Mayo Science PosterSymposium 2010; Northern New
Mexico College, Espanola New Mexico.
New Mexico 3% ScholarsScholarship-TheUniversity of New Mexico- $1800/Fall-Spring
2013-2014
Kraft EngineeringGraduateScholarship-TheUniversity of New Mexico- $3000/Fall-
Spring 2013- 2014
SecondPlaceBioscienceTechnical TalkAward-Omic Analysis forToxicology;Los
Alamos National Laboratory Student Symposium; August 2014.
LANL 2014DistinguishedPerformanceAward Recipient-ProjectATHENAteam.
http://int.lanl.gov/employees/compensation/awards/dpa/large-teams.shtml
GraduateSuccess Scholarship-TheUniversity of New Mexico Graduate Success
Scholarship- Excellencein academic achievement. $9000 award. Spring -2015
Review and Advisory Activities
 American Society of Mass Spectrometry;Member 2007-2009
 Biology Interest Group (BIG),Northern New Mexico College; Member 2008-2009
 National Association of Corrosion Engineers (NACE); Member 2009-2010
 Vice-President Governing Council Board of La JicaritaCharter Schools, Penasco, NM-
2011-2012.

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 Los Alamos National Laboratory-Bioscience Division-WorkerSafety and Security Team
(WSST) Student Representative. 2013-2014
References
Available upon request