Forensic medicine medical negligence 2-bolam principle
Biochem pcr (29.6.10)
1. Double strand cDNA AAAAA TTTTT RT RT RT Oligo dT primer is bound to mRNA Reverse transcriptase (RT) copies first cDNA strand Reverse transcriptase digests and displaces mRNA and copies second strand of cDNA Conversion of mRNA to cDNA by Reverse Transcription AAAAA TTTTT AAAAA TTTTT
2. A. Double strand DNA 50 º Taq Taq B. Denature 96 º C. Anneal primers 50 º D. Polymerase binds 72 º
3. Taq Taq E. Copy strands First round of cDNA synthesis (4 strands) Taq Taq 72 º 1 2 3 4 F. Denature 96 º