This paper studies the effects of bi-directional ground excitation on the seismic response of an isolated bridge. Nonlinear dynamic analysis and pushover analysis are used to analyze a 3-span continuous bridge isolated using various bearing types under uni-directional and bi-directional ground motions. The results show that bi-directional excitation can significantly increase the bridge response, especially for friction pendulum isolated bridges, where uni-directional analysis underestimates the response. Pushover analysis provides conservative estimates of response compared to dynamic analysis for elastomeric bearings but underestimates response for friction-based systems. The maximum bi-directional response does not coincide with the maximum uni-directional response, indicating the need to consider