Culture media

BACTERIAL NUTRITION & CULTURE MEDIA G.HARIPRASAD M.Sc.Med micro M.Phil LECTURER THOOTHUKUDI GOVT. MEDICAL COLLEGE THOOTHUKUDI

BASIC NUTRITIONAL REQUIREMENTS ,[object Object],[object Object],[object Object],[object Object],[object Object]

GASEOUS REQUIREMENTS ,[object Object],[object Object]

TEMPERATURE ,[object Object],[object Object],[object Object]

H-ION CONCENTRATION (pH) ,[object Object],[object Object],[object Object]

CULTURE MEDIA BASIC INGREDIENTS ,[object Object],[object Object],[object Object],[object Object]

TYPES OF CULTURE MEDIA ,[object Object]

SIMPLE OR BASAL MEDIUM ,[object Object],[object Object],[object Object],NUTRIENT AGAR

COMPLEX MEDIUM ,[object Object],[object Object]

SYNTHETIC & SEMISYNTHETIC MEDIUM ,[object Object],[object Object]

SPECIAL MEDIUM ENRICHED MEDIUM ,[object Object],[object Object],[object Object],BLOOD AGAR CHOCOLATE AGAR

GAMMA HEMOLYSIS ALPHA HEMOLYSIS WITH GREENISH DISCOLORATION (no clear zone around colonies) BETA HEMOLYSIS (clear zone around colonies)

BETA HEMOLYSIS UNINOCULATED BLOOD AGAR

BROWN PIGMENT ON BLOOD AGAR PORPHYROMONAS / PREVOTELLA SP., - IMPORTANT DENTAL PATHOGEN

CHOCOLATE AGAR (HEATED BLOOD AGAR)

SPECIAL MEDIUM ENRICHMENT MEDIUM ,[object Object],[object Object],[object Object]

TCBS AGAR – FOR VIBRIO CHARCOAL AGAR – FOR BORDETELLA DCA – FOR SALMONELLA MSA – FOR STAPH

EOSIN METHYLENE BLUE – E.COLI

LOWENSTEIN –JENSEN (LJ) MEDIUM SELECTIVE MEDIUM FOR MYCOBACTERIUM TUBERCULOSIS

SPECIAL MEDIUM SELECTIVE MEDIUM ,[object Object],[object Object],[object Object]

SPECIAL MEDIUM DIFFERENTIAL MEDIUM ,[object Object],[object Object]

LACTOSE FERMENTERS (PINK COLONIES) LACTOSE FERMENTERS NON - LACTOSE FERMENTERS

MACCONKEY AGAR NON-LACTOSE FERMENTER (PALE OR COLORLESS) LACTOSE FERMENTER (PINK COLOUR)

MAC CONKEY AGAR NUTRIENT AGAR BLOOD AGAR PINK COLOUR (TRANSPARENT) DARK RED COLOUR (OPAQUE) PALE YELLOW COLOUR (TRANSPARENT)

SPECIAL MEDIUM TRANSPORT MEDIUM ,[object Object],[object Object]

SPECIAL MEDIUM ANAEROBIC MEDIUM ,[object Object],[object Object],[object Object]

ROBERTSON COOKED MEAT BROTH THIOGLYCOLLATE BROTH

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