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Similaire à 2. Friday Monogastric Sessions dr amir ghane danisco animal nutrition - probiotics and nutribiosis csi latest and new concepts
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2. Friday Monogastric Sessions dr amir ghane danisco animal nutrition - probiotics and nutribiosis csi latest and new concepts
- 1. FEEDWORKS CONFERENCE 2018 FEEDING THE FUTURE USING NUTRIBIOSIS DR. AMIR E. GHANE, PH.D.,PAS. DUPONT ANIMAL NUTRITION 1
- 2. REGULATION OF ANTIBIOTIC GROWTH PROMOTERS (AGP) WILL ACCELERATE 2 Source:DuPontinterpretationofregional/countryregulatoryframeworks Existing ban AGP regulatory status in leading markets Existing restrictions - scheduled ban No restrictions Existing restrictions + customer-driven self regulation
- 3. DUPONT, AS A TECHNOLOGY AND SCIENCE LEADER, IS STRATEGICALLY FOCUSED ON GUT HEALTH AND ALIGNMENT WITH CUSTOMERS 3April 12, 2018 Training Nutribiosis April 12 Confidential Building on our extensive, in-depth understanding of feed additive applications Microbiome research In-field Analysis and Applied Research, with 80,000 guts sampled from 500 poultry farms Advanced omics technology, In vitro Gut Simulators Let’s collaborate to make change
- 4. ANTIBIOTIC-FREE ANIMAL PRODUCTION IS A COMPLEX ISSUE 4 Maintaining consistency is a daily challenge for live production managers and nutritionists are overwhelmed by the number of different feed options to test. There is no silver bullet Understanding the interaction between the gut & immune function, microbiome and nutrition and how new technologies influence this is a crucial step in this evolution Unpredictable disease challenges are on the rise with veterinarians having fewer weapons to fight them
- 5. Gut and immune function NUTRIBIOSIS IS A STATE DESCRIBING AN INTERACTION 5 Nutrition Microbiome
- 6. FAVORABLE NUTRIBIOTIC STATE = GOOD INTESTINAL HEALTH & OPTIMAL PERFORMANCE MicrobiomeGut and immune function Nutrition 6
- 7. UNFAVORABLE NUTRIBIOTIC STATE = POOR INTESTINAL HEALTH & SUBOPTIMAL PERFORMANCE MicrobiomeGut and immune function Nutrition 7
- 8. ANTIBIOTIC-USE SUPPORTED A NUMBER OF CELLULAR FUNCTIONS THROUGH DIRECT AND INDIRECT MECHANISMS 810/18/2018 Change this: Go to Insert > Header & Footer • Reduced disease challenge • Reduced sub-clinical disease • Reduced outbreaks • Reduced bacterial dysbiosis events • Direct signaling with host tissue • Off-target anti-inflammatory effects • Lowered biosecurity hurdle • Better synchronized growth without pathogen challenge Effects of antibiotics are still governed by natural physiology and gut-microbe interactions; therefore, we can identify natural stability points through nutrition, gut microbiome, and host physiology to substitute effects of exogenous antibiotics.
- 9. THE UNDERSTANDING OF NUTRIBIOSIS BRINGS NEW INSIGHTS TO ANIMAL PRODUCERS, ENABLING THEM TO: 9April 12, 2018 Training Nutribiosis April 12 Confidential Improving Animal Performance Promote Animal Welfare Improving Gut Health Proper decision making on feed additives
- 10. FOCUS ON CASE-STUDIES: RECAPTURING PERFORMANCE FROM UNFAVORABLE NUTRIBIOTIC STATES 1010/18/2018 Change this: Go to Insert > Header & Footer
- 11. UNFAVORABLE NUTRIBIOTIC STATE GIT UNDER CHALLENGE (NECROTIC ENTERITIS) Nutrition High feed intake High levels of anti-nutritional factors Poor ingredient quality High levels of undigested nutrients Microbiome Cocci outbreak Presence of toxin producing C. perfringens Low diversity/ imbalance High levels of protein fermenters Gut and immune function Inflammation Oxidative stress Reduced surface area Poor gut function Ultimately these factors will interact with each other reducing animal performance: 11
- 12. Ultimately these factors will interact with each other reducing animal performance: UNFAVORABLE NUTRIBIOTIC STATE SWINE DYSENTERY Nutrition High energy high protein diets Low nutrient digestibility Too much or too little carbohydrate fermentation in the hind gut Microbiome Increased prevalence of Brachyspira hyodysenteriae Gut and immune function B. hyodysenteriae induces extensive inflammation Necrosis of the epithelial surface of the caecum and colon 12
- 13. ANTIBIOTIC TREATMENTS CAN SUCCESSFULLY TREAT PATHOGEN-DERIVED CHALLENGES, BUT AT WHAT COST? 1310/18/2018 Change this: Go to Insert > Header & Footer • Antibiotic resistance • Bacteriophage release and genetic exchange • No coverage of root cause (potential reservoir of pathogens in the system) Antibiotic resistance requires multiple antibiotic treatments and loss of drugs or classes for use in field Genes for virulence or resistance can be quickly passed through to other bacteria after bacteriophages released Reservoirs of pathogens can continually re-infect animals in environment
- 14. ANTIBIOTICS CAN ATTACK PATHOGENS DIRECTLY, BUT MAY NOT PROVIDE SYSTEMS-WIDE COVERAGE 1410/18/2018 Change this: Go to Insert > Header & Footer Time Bacterialchallenge Normal trajectory of infection Active infection (toxins, iron capture, cellular destruction) Active immune response (phagocytosis and cytokine signaling) Re-alignment of energy resources (Dynamic energy budget) Understanding nutribiosis can play a part in developing your feeding strategy to support these host functions during infection, and also help to support natural recovery post-infection Distorted microbiome
- 15. GIVEN THE SUSCEPTIBILITY OF HOST SYSTEMS DURING INFECTION, HOW CAN STRATEGY COME TOGETHER TO HELP PROMOTE HEALTH AND IMPROVE PERFORMANCE ? 1510/18/2018 Change this: Go to Insert > Header & Footer
- 16. Nutrition Improved digestibility of starch fat and protein Improved nutrient uptake Improved energy utilization shown by improved calorie conversion into growth performance Improve breakdown of fiber in the diet FAVORABLE NUTRIBIOTIC STATE Syncra® AVI Ultimately these factors will interact with each other to give positive effects: Gut and immune function Improved intestinal integrity and tight junctions Improved villi height to maximize absorptive area for nutrient absorption Reduced risk of gut inflammation and inflammatory response Reduced physiological stress Microbiome Production of prebiotic AXOs Increased beneficial bacterial populations such as Lactobacillus versus non-beneficial populations Reduced levels of opportunistic microbes (like coliforms) Positive changes in fermentation patterns (increased butyrate production) 16
- 17. SYSTEMS-WIDE COVERAGE OF Syncra® AVI 1710/18/2018 Change this: Go to Insert > Header & Footer
- 18. FAVORABLE NUTRIBIOTIC STATE Syncra® SWI Gut and immune function Improved gut barrier strength through tighter junctions Nutrition Release of fiber bound nutrients Increased protein digestion and amino acid utilization Microbiome Bacillus adapt to the diet and produce fiber degrading enzymes to help nutrient digestion Reduction in undigested proteins so less substrate for non-beneficial bacteria Production of prebiotic AXOs Reduced protein fermentation so less ammonia produced Increase in Lactobacillus populations, indicating a beneficial shift in the microbiota Increase fiber fermentation resulting in higher SCFA (e.g. butyrate). Ultimately these factors will interact with each other to give positive effects: 18
- 19. SYSTEMS-WIDE COVERAGE OF Syncra® SWI 1910/18/2018 Change this: Go to Insert > Header & Footer
- 20. DUPONT PRODUCTS PROVEN TECHNOLOGIES FOR MAXIMISING NUTRIENT DIGESTION, GUT HEALTH AND PERFORMANCE Microbial modulation Maximised nutrient digestibility Improved intestinal integrity Immune response modulation DuPont enzymes Improved digestibility and performance Improved flock uniformity Flexibility on ingredient use DuPont probiotic Protection against health challenges Accelerated immune development Proven efficacy on >500 farms
- 21. ARABINOXYLANS ARE IN BOTH SOLUBLE AND INSOLUBLE FRACTIONS OF THE CELL WALL 4.48% 3.62% 3.02% 6.51% 1.50% 0.37% 0.76% 1.17% Wheat Corn SBM Corn DDGS Ins. Arabinoxylans S. Arabinoxylans Different raw materials have different arabinoxylan composition 21
- 22. TOTAL ARABINOXYLAN (TAX) LEVELS IN BROILER DIETS IMPACTS PERFORMANCE Trialcode:XAP0910; XAP1408;XAP1409 1.73 1.70 1.83 1.75 1.54 1.54 1.59 1.55 1.591.57 1.55 1.51 y = 0.4669x + 1.0021 R² = 0.60 1.4 1.5 1.6 1.7 1.8 1.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 FCR 42d TAX:ME (kg/kcal*10-4) Impact on increasing levels of TAX in diets on performance in broilers* *Combined data of positive and negative control diets without addition of enzymes, TAX calculated with decision tree calculator divided ME of diet for normalization 22
- 23. MOVING FORWARD IN GUT HEALTH STRATEGIES 2310/18/2018 Change this: Go to Insert > Header & Footer Where are you leveraging your production system based on feed and performance? How is your strategy providing coverage to not only gut tissue, but also the immune system? Changes to the microbiome can happen fast and may not self-correct, how can you stabilize against a stressed system?
- 24. Questions? 24
- 26. Copyright © 2018 DuPont. All rights reserved. The DuPont Oval Logo and the Leaf Globe are trademarks or registered trademarks of E. I.du Pont de Nemours and Company or its affiliates. Nothing contained herein shall be construed as a representation that any recommendations, use or resale of the product or process described herein is permitted and complies with the rules or regulations of any countries, regions, localities, etc., or does not infringe upon patents or other intellectual property rights of third parties. The information provided herein is based on data DuPont believes to be reliable, to the best of its knowledge and is provided at the request of and without charge to our customers. Accordingly, DuPont does not guarantee or warrant such information and assumes no liability for its use. If this product literature is translated, the original English version will control and DuPont hereby disclaims responsibility for any errors caused by translation. This document is subject to change without further notice.
Notes de l'éditeur
- ***ONLY READ FIRST PARGRAPH – REST IS BACKGROUND INFO*** There has never been a more urgent need for this understanding. The rise of antimicrobial resistance combined with increasing global pressure to reduce or remove antibiotics from the food chain is driving fundamental change within the animal production industry; creating new challenges and heightening demand for viable alternatives. Existing Ban: EU, Thailand, Russia, S. Korea, Australia. Note: therapeutic use of antibiotics is not curtailed. Existing restrictions: China, US, Canada, NZ: no “ban” but the products can only be used under veterinary supervision and/or to treat disease (i.e. quasi ban). i.e. growth promotion claims have been removed and prescriptions are required for use in animals, but it’s not called a “ban”. Latam: regulated as veterinary products – can be registered, but the situation is that all LATAM is migrating to ban the use as growth promoters Scheduled ban: Vietnam ban scheduled for 2019 – pending and may change but currently scheduled. No restrictions: Malaysia, Philippines Drivers of regulation: Antimicrobial use in livestock is primarily associated with the routine use of antimicrobials as growth promoters or their inappropriate use as low-cost substitutes for hygiene measures that could otherwise prevent infections in livestock. In 2016, the UN recognized the inappropriate use of antimicrobials in animals as a leading cause of rising anti-microbial resistance. Causes of AMR: The primary driver for the accumulation of harmful resistance genes in the animal reservoir is the large quantity of antimicrobials used in animal production. since the biomass of animals raised for food exceeds by far the biomass of humans, new resistant mutations are more likely to arise in animals. Drivers of customer driven self regulation: Consumer groups are beginning to demand action on antibiotic use. For example, in the USA: McDonalds, Chick Filet all publicly committed to going anti-biotic free. Advocacy opportunities: Regulators are struggling with how to regulate traditional feed additive type products with drug-like claims. We see more and more associations/regulatory/quasi-govt bodies talking about this issue We need to be active in our advocacy efforts, provide regulators with the information they need.
- Why DuPont? As leaders in both enzyme and gut health technologies, DuPont is breaking open the “black box” of nutribiosis, leveraging our microbiome research, our in-field analysis and applied research of over 80,000 guts sampled from over 500 farms, advanced omics technology and in vitro gut simulators, as well as continuing to build on our extensive, in-depth understanding of feed additive applications. DuPont Why – DuPont exists to collaborate with customers to make positive change
- Without the antibiotic line of defense, animal production becomes more of a complex issue, as unpredictable and diverse disease challenges are on the rise, with veterinarians having fewer weapons to fight them. Maintaining consistency is a daily challenge for live production managers and nutritionists are simply overwhelmed by the number of different feed alternatives to test. All recognize that there is no silver bullet. So understanding the interaction between the gut and immune function, microbiome and nutrition, and how new technologies like ways to measure this interaction influence this, is a crucial step in this evolution. We have seen that when it comes to evaluating the impact of any nutritional intervention on animal health, there remains significant room for improvement. Current tools for measuring feed additive efficacy are still heavily relying on traditional feed and weigh metrics, such as feed conversion ratios (FCR) and average daily gain (ADG). However, to properly measure nutribiosis, producers need additional scientific measurements on site which take into account a multi-disciplinary approach to demonstrating added value. At DuPont, we are committed to improving these tools.
- Nutribiosis is the state describing the interaction between nutrition, microbiome and gut & immune function. Now let’s look at why the interaction is important. These functional areas – nutrition, microbiome and gut and immune function are not new, however looking at them together in a holistic way and understanding how they interact and impact each other is crucial and what makes nutribiosis different from other gut health concepts in the market. It is based on 3 principles: Firstly, we need to get nutrition right to ensure that the animal has all the nutrients needed to reach its full potential. It is also important to either limit the amount of undigested nutrients or control the types of undigested nutrients that reach the terminal ileum, so that we are not providing substrates for the non-beneficial bacterial populations to feed and thrive on. Secondly, having a microbiome which is diverse, where beneficial bacteria outnumber non-beneficial bacteria, and possible opportunistic pathogens are kept under control is key. This avoids the occurrence of sub-clinical and clinical disease challenges that would otherwise reduce overall performance and profitability. Finally, a well-developed gut structure and high-functioning immune system are vital to allow animals to maximize nutrient absorption and deal with challenges when they occur - whether they are bacterial, viral or environmental. Any immune response mounted by the animal uses energy and can negatively impact growth if the immune response is sustained or uncontrolled. *Microbiome: is the population of microorganisms located in a specific organ, in this case GIT. Microbiome covers everything the microbiota is doing; where microbiota is the microbes which are present and microbiome is the entire capability of the microbiome.
- A Favourable nutribiotic state is what we consider as good intestinal health The trinity equilibrium is fragile and can also be disturbed by external and unpredictable factors, i.e. climate, temperature, virus. That’s the reason why gut health additives should always be added in the background to secure the equilibrium.
- Nutribiosis is a state And as such It can be favourable or unfavourable In the case of an unfavourable nutribiotic state, we see an imbalance between the 3 pillars: nutrition, microbiome and gut & immune function. This imbalance results in poor intestinal health and suboptimal performance. The equilibrium between the three pillars of nutribiosis is fragile and there are many external factors that could make it go off balance. This typically leads to a vicious cycle from which the animal or its performance may never fully recover.
- It’s crucial because this understanding of nutribiosis brings new insights to animal producers, enabling them to improve gut health and make more profitable decisions, leading to improved animal performance and welfare.
- So lets look at some case studies involving unfavorable nutribiotic states that were generated by infection. Traditionally these may currently or were once treated by simple antibiotics, but the overall interactions were not limited to bacteria causing infection.
- The example of an unfavourable nutribiotic state for broilers that we have chosen is Necrotic Enteritis. Necrotic enteritis is a condition which arises from a combination of an intestinal challenge and an overload of nutrients in the latter part of the intestinal tract The intestinal challenge can be caused by coccidiosis, this combined with high levels of undigested protein (as a result of high levels of feed intake, or high levels of antinutritional factors) can result in an outbreak The outbreak is caused by an opportunistic pathogen, Clostridium perfringens – which thrives on undigested nutrients C. perfringens causes intestinal damage through the production of toxins, reducing gut function by reducing the surface area for absorption of nutrients Which in turn creates more feedstuffs for the bacteria – it is for this reason that we consider necrotic enteritis to be a vicious cycle Ultimately these interactions between nutrition, microbiome and gut and immune function result in suboptimal animal performance
- The example of an unfavourable nutribiotic state for pigs that we have chosen is Swine Dysentery. 1. High energy high protein diets are associated with swine dysentery as well as low nutrient digestibility and too much or too little carbohydrate fermentation in the hind gut 2. Combined with poor management practices nutritional factors can cause increased prevalence of Brachyspira hyodysenteriae, a causative agent of swine dysentery. 3. B. hyodysenteriae induces extensive inflammation and necrosis of the epithelial surface of the caecum and colon Ultimately these interactions between nutrition, microbiome and gut and immune function result in suboptimal animal performance Ref…http://www.mdpi.com/1660-4601/10/5/1927/htm
- To illustrate the positive nutribiotic state we have looked at 3 examples from our product portfolio and how those 3 products can result in a positive nutribiotic state. These are examples only and other products could also be used. The first product we will look at is Syncra® AVI, a product developed for health effects, and how it positively influences nutrition, the microbiome and gut and immune function. Firstly lets consider Gut and immune function. Studies have shown that Syncra® AVI: Improved intestinal integrity and tight junctions leading to less risk of foreign materials such as microbes crossing between the gut cells and into the blood. Improved villi height to maximise absorptive area for nutrient absorption Reduced risk of gut inflammation and inflammatory response following challenge indicating the animal recovers quicker Reduced physiological stress demonstrated by reductions in acute phase protein levels following challenge. If we now look at nutritional effects of the product, in trials we have seen: Improved digestibility of starch fat and protein meaning less undigested substrate reaching the terminal ileum where it can act as food for non-beneficial bacteria Improved nutrient uptake Improved energy utilisation shown by improved calorie conversion into growth performance Improve breakdown of fibre in the diet Finally lets consider the effects on the microbiome: The product contains xylanase which results in production of prebiotic AXOs Increased beneficial bacterial populations such as Lactobacillus versus non-beneficial populations Reduced levels of opportunistic microbes (like coliforms). Less undigested protein reaching the terminal ileum means less substrates for protein fermenters like Clostridia. Positive changes in fermentation patterns, illustrated by increased butyrate production in in vitro studies
- Next we will look at Syncra® SWI, a product developed for performance improvement, and how it positively influences nutrition, the microbiome and gut and immune function. First looking at gut and immune function trials, we have demonstrated improved gut barrier through tight junctions – reducing the risk of materials passing between the gut cells and causing an immune response. Second, considering the nutritional effects the product has been designed and proven to release fibre bound nutrients and increase protein digestion and amino acids utilization resulting in better growth of the animal and better carcass characteristics such as increased lean meat deposition. Increased protein digestion means less undigested protein reaching the hind gut of the animal. Finally, looking at the microbiome, we have seen a number of benefits with the product: Bacillus adapt to the diet and produce fibre degrading enzymes to help nutrient digestion Less substrate for non-beneficial bacteria due to the better digestion seen with the product Production of prebiotic AXOs due to the fibre degrading enzymes produced by the Bacillus Reduced protein fermentation so less ammonia produced Increase in Lactobacillus populations, indicating a beneficial shift in the microbiota Increased fibre fermentation resulting in higher SCFA (e.g butyrate).
- The Syncra® AVI product combines enzyme technology for breaking down antinutrients and improving nutrient digestibility and probiotic technology for improving gut health, creating a new benchmark for optimizing gut health and maximising performance. With this combination we have demonstrates effects on digestibility, the microbiome, as well as intestinal integrity, gut structure and immune responses.
- Arabinoxylans are polysaccharides formed by a backbone made of xylose and branched arabinose (sugar monomers). This polysaccharide is the main NSP (non starch polysaccharide) in corn and wheat and is part of the fiber in most of the vegetable raw materials. Its degree of branching and polymerization (DS, DP) gives arabinoxylans different behaviour in water. More branched and bigger polymers (higher DS and DP) are usually insoluble in water and cause the boxing effect by acting as a barrier to the animal’s endogenous enzymes. While smaller polymers are water soluble but increases the digesta viscosity reducing the absorption rate of nutrients during digestion.