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By – Annu Maurya
 Microbiology (from Greek µῑκρος, mīkros, "small";
βίος, bios, "life"; and -λογία, -logia) is the study of
microorganisms, which are unicellular or cell-
cluster microscopic organisms. This includes
eukaryotes such as fungi and protists, and
prokaryotes. Viruses, though not strictly classed as
living organisms, are also studied. In short;
microbiology refers to the study of life and
organisms that are too small to be seen with the
naked eye.
 Microbiology typically includes the study of the
immune system, or Immunology. Generally,
immune systems interact with pathogenic microbes;
these two disciplines often intersect which is why
many colleges offer a paired degree such as
"Microbiology and Immunology
 Microbiology is a broad term which includes
virology, mycology, parasitology, bacteriology and
other branches. A microbiologist is a specialist in
microbiology

 The existence of microorganisms was hypothesized
for many centuries before their actual discovery in
the 17th century. In 600 BCE, the ancient Indian
surgeon Susruta held microbes responsible for
several diseases and explained in Sushruta Samhita
that they can be transmitted through contact, air or
water.
 Bacteria, and other microorganisms, were first
observed by Antonie van Leeuwenhoek in 1676
using a single-lens microscope of his own design. In
doing so Leeuwenhoek made one of the most
important discoveries in biology and initiated the
scientific fields of bacteriology and microbiology.
The name "bacterium" was introduced much later,
by Ehrenberg in 1828, derived from the Greek
βακτηριον meaning "small stick". While Van
Leeuwenhoek is often cited as the first
microbiologist, the first recorded microbiological
observation, that of the fruiting bodies of molds,
was made earlier in 1665 by Robert Hooke
 The field of microbiology can be generally divided
into several subdisciplines
 Microbial physiology: The study of how the
microbial cell functions biochemically. Includes the
study of microbial growth, microbial metabolism
and microbial cell structure.
 Microbial genetics: The study of how genes are
organized and regulated in microbes in relation to
their cellular functions. Closely related to the field
of molecular biology.
 Cellular microbiology: A discipline bridging
microbiology and cell biology.
 Medical microbiology: The study of the
pathogenic microbes and the role of microbes
in human illness. Includes the study of
microbial pathogenesis and epidemiology and
is related to the study of disease pathology and
immunology.
 Veterinary microbiology: The study of the role
in microbes in veterinary medicine or animal
taxonomy.
 Environmental microbiology: The study of the
function and diversity of microbes in their natural
environments. Includes the study of microbial
ecology, microbially-mediated nutrient cycling,
geomicrobiology, microbial diversity and
bioremediation. Characterisation of key bacterial
habitats such as the rhizosphere and phyllosphere,
soil and groundwater ecosystems, open oceans or
extreme environments (extremophiles).
 Evolutionary microbiology: The study of the
evolution of microbes. Includes the study of
bacterial systematics and taxonomy
 Industrial microbiology: The exploitation of
microbes for use in industrial processes. Examples
include industrial fermentation and wastewater
treatment. Closely linked to the biotechnology
industry. This field also includes brewing, an
important application of microbiology.
 Aeromicrobiology: The study of airborne
microorganisms.
 Food microbiology: The study of microorganisms
causing food spoilage and foodborne illness. Using
microorganisms to produce foods, for example by
fermentation.
 Pharmaceutical microbiology: the study of
microorganisms causing pharmaceutical
contamination and spoil
 Agricultural microbiology: The study of
agriculturaly important microorganisms
 Fishery Microbiology: Fisheries microbiology is
that branch of science which deals with the study of
a vast collection of microscopic, unicellular and
largely undifferentiated life from of microscopic
living organism and their effect on the fish and fish
culture
 The bacteria whose proper home is in water and
which can developed optimally only in water.
 Other sources of Bacteria in Water
Besides genuine aquatic bacteria, a number of bacteria
from other habitates are also found.
 Soil: Water in close contact with soil
 Air: A constant rain of bacteria falls from the air on
to surface waters Plants, animals and human
 bacteria living in the sea are different from those in
fresh water and amongst the latter those of the rivers
are different from those in lakes.
 Majority of aquatic bacteria are heterophilic, i.e. they
live on organic substances.
 Some are photo and chemoautotrophic needing only
inorganic nutrients.
 Morphologically, most aquatic bacteria are similar to
the basic types of terrestrial bacteria.
 In most water predominant bacteria are gram (-).
 The majority of aquatic bacteria are motile, as a rule by
means of flagella.
 Genuine aquatic bacteria are distinguished by their
ability to utilize very small concentration of nutrients.
 Bacteria may live in the water free or growing on
some solid substratum.
 Systematically aquatic bacteria are not a
homogenous group, their representatives are found
in almost all orders of the class of Bacteria.
 There are great biological differences between
inland and marine bacteria.
Bacteria in inland waters -There are relationships
between the bacterial flora of inland waters and that
of the soil.
Ground water -Ground water is poor in micro-
organisms and in nutrients. Ground water
from different parts of the world examined
included the microorganisms belonging to the
genera:- Achromobacter, Flavobacterium,
Micrococcus, Nocardia, Cytophaga,
Hypomicrobium, Planctomyces, Gallionella,
Caulobacter, Agrobacterium, Clostridium etc.
 Spring Water- Spring water contains more or less
similar type of bacteria as mentioned above. Other
bacteria added are iron containing water contain
iron bacteria (Gallionella ferrugina, Leptothrix
ochrcea etc.), thermal springs contain
thermotolerant and thermophilic species (Sulfolobus
acidocaldarius, Leptothrix thermalis, Thermus
aquaticus etc.)
 Streams In streams which are poor in nutrients,
gram negative non sporeing rod shaped
predomonate which include- Psedomonas,
Flavobacterium, Acinetobacter, Moraxella etc. with
increasing eutrophication Bacillus and
Enterobacteria gain importance
 River Depending on the sewage load, rivers carry
more or less numerous sewage bacteria which
include Escherichia coli, Proteus vulgaris,
Salmonella sp, Clostridium, Desulfovibrio
desulfuricans etc.
 Lakes-- Non sporing rod shaped bacteria
predominant in the lakes of temperate climate.
Looking through the literature, it can be gathered
that bacteria particularly of the genera
Achromobacter, Flavobacterium, Vibrio,
Brevibacterium, Spirillum, Microccus, Sarcina,
Bacillus, Pseudomonas, Nocardia, Streptomyces and
Cytophaga occur widely in lakes. Majority of
bacteria living in salt lakes are halophilic and
halotolerance forms (Halobacterium and
Halococcus).

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General microbiolgy

  • 1. By – Annu Maurya
  • 2.  Microbiology (from Greek µῑκρος, mīkros, "small"; βίος, bios, "life"; and -λογία, -logia) is the study of microorganisms, which are unicellular or cell- cluster microscopic organisms. This includes eukaryotes such as fungi and protists, and prokaryotes. Viruses, though not strictly classed as living organisms, are also studied. In short; microbiology refers to the study of life and organisms that are too small to be seen with the naked eye.
  • 3.  Microbiology typically includes the study of the immune system, or Immunology. Generally, immune systems interact with pathogenic microbes; these two disciplines often intersect which is why many colleges offer a paired degree such as "Microbiology and Immunology  Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology and other branches. A microbiologist is a specialist in microbiology
  • 4.   The existence of microorganisms was hypothesized for many centuries before their actual discovery in the 17th century. In 600 BCE, the ancient Indian surgeon Susruta held microbes responsible for several diseases and explained in Sushruta Samhita that they can be transmitted through contact, air or water.
  • 5.  Bacteria, and other microorganisms, were first observed by Antonie van Leeuwenhoek in 1676 using a single-lens microscope of his own design. In doing so Leeuwenhoek made one of the most important discoveries in biology and initiated the scientific fields of bacteriology and microbiology. The name "bacterium" was introduced much later, by Ehrenberg in 1828, derived from the Greek βακτηριον meaning "small stick". While Van Leeuwenhoek is often cited as the first microbiologist, the first recorded microbiological observation, that of the fruiting bodies of molds, was made earlier in 1665 by Robert Hooke
  • 6.  The field of microbiology can be generally divided into several subdisciplines  Microbial physiology: The study of how the microbial cell functions biochemically. Includes the study of microbial growth, microbial metabolism and microbial cell structure.  Microbial genetics: The study of how genes are organized and regulated in microbes in relation to their cellular functions. Closely related to the field of molecular biology.
  • 7.  Cellular microbiology: A discipline bridging microbiology and cell biology.  Medical microbiology: The study of the pathogenic microbes and the role of microbes in human illness. Includes the study of microbial pathogenesis and epidemiology and is related to the study of disease pathology and immunology.  Veterinary microbiology: The study of the role in microbes in veterinary medicine or animal taxonomy.
  • 8.  Environmental microbiology: The study of the function and diversity of microbes in their natural environments. Includes the study of microbial ecology, microbially-mediated nutrient cycling, geomicrobiology, microbial diversity and bioremediation. Characterisation of key bacterial habitats such as the rhizosphere and phyllosphere, soil and groundwater ecosystems, open oceans or extreme environments (extremophiles).  Evolutionary microbiology: The study of the evolution of microbes. Includes the study of bacterial systematics and taxonomy
  • 9.  Industrial microbiology: The exploitation of microbes for use in industrial processes. Examples include industrial fermentation and wastewater treatment. Closely linked to the biotechnology industry. This field also includes brewing, an important application of microbiology.  Aeromicrobiology: The study of airborne microorganisms.
  • 10.  Food microbiology: The study of microorganisms causing food spoilage and foodborne illness. Using microorganisms to produce foods, for example by fermentation.  Pharmaceutical microbiology: the study of microorganisms causing pharmaceutical contamination and spoil  Agricultural microbiology: The study of agriculturaly important microorganisms
  • 11.  Fishery Microbiology: Fisheries microbiology is that branch of science which deals with the study of a vast collection of microscopic, unicellular and largely undifferentiated life from of microscopic living organism and their effect on the fish and fish culture
  • 12.  The bacteria whose proper home is in water and which can developed optimally only in water.  Other sources of Bacteria in Water Besides genuine aquatic bacteria, a number of bacteria from other habitates are also found.  Soil: Water in close contact with soil  Air: A constant rain of bacteria falls from the air on to surface waters Plants, animals and human  bacteria living in the sea are different from those in fresh water and amongst the latter those of the rivers are different from those in lakes.
  • 13.  Majority of aquatic bacteria are heterophilic, i.e. they live on organic substances.  Some are photo and chemoautotrophic needing only inorganic nutrients.  Morphologically, most aquatic bacteria are similar to the basic types of terrestrial bacteria.  In most water predominant bacteria are gram (-).  The majority of aquatic bacteria are motile, as a rule by means of flagella.  Genuine aquatic bacteria are distinguished by their ability to utilize very small concentration of nutrients.
  • 14.  Bacteria may live in the water free or growing on some solid substratum.  Systematically aquatic bacteria are not a homogenous group, their representatives are found in almost all orders of the class of Bacteria.  There are great biological differences between inland and marine bacteria.
  • 15. Bacteria in inland waters -There are relationships between the bacterial flora of inland waters and that of the soil. Ground water -Ground water is poor in micro- organisms and in nutrients. Ground water from different parts of the world examined included the microorganisms belonging to the genera:- Achromobacter, Flavobacterium, Micrococcus, Nocardia, Cytophaga, Hypomicrobium, Planctomyces, Gallionella, Caulobacter, Agrobacterium, Clostridium etc.
  • 16.  Spring Water- Spring water contains more or less similar type of bacteria as mentioned above. Other bacteria added are iron containing water contain iron bacteria (Gallionella ferrugina, Leptothrix ochrcea etc.), thermal springs contain thermotolerant and thermophilic species (Sulfolobus acidocaldarius, Leptothrix thermalis, Thermus aquaticus etc.)
  • 17.  Streams In streams which are poor in nutrients, gram negative non sporeing rod shaped predomonate which include- Psedomonas, Flavobacterium, Acinetobacter, Moraxella etc. with increasing eutrophication Bacillus and Enterobacteria gain importance  River Depending on the sewage load, rivers carry more or less numerous sewage bacteria which include Escherichia coli, Proteus vulgaris, Salmonella sp, Clostridium, Desulfovibrio desulfuricans etc.
  • 18.  Lakes-- Non sporing rod shaped bacteria predominant in the lakes of temperate climate. Looking through the literature, it can be gathered that bacteria particularly of the genera Achromobacter, Flavobacterium, Vibrio, Brevibacterium, Spirillum, Microccus, Sarcina, Bacillus, Pseudomonas, Nocardia, Streptomyces and Cytophaga occur widely in lakes. Majority of bacteria living in salt lakes are halophilic and halotolerance forms (Halobacterium and Halococcus).