2. Infertility is defined as inability of couples to achieve
pregnancy following one year of unprotected intercourse.
Primary infertility
Secondary infertility
3. Male infertility may be due to:
1. Decrease in the number of sperms
2. Sperm being blocked from being released
3. Sperm that do not work properly
Other causes of male infertility…..?
4. Semen analysis…………………….male pathology.
Techniques for andrology for monitoring semen
parameters, like sperm concentration, motility and
morphology.
Computer assisted semen analysis (CASA)
5. CASA has categorized motility
(micrometer/sec), of sperm into three classes
as:
1. average path velocity (VAP)
2. straight line velocity (VSL)
3. curvilinear velocity (VCL)
6. Testicular pathology is divided in to subgroups as:
1. Teratozoospermia
2. Oligozoospermia
3. Asthenozoospermia
4. Azoospermia
5. Polyzoospermia
7. Antisperm antibodies are antibodies directed against the
sperm.
Research began in 1899
Found systematically and in local secretion.
Impair the fertilizing ability of spermatozoa.
8. To compare the data of semen parameters from fertile
and infertile subject categories.
To see the role of antisperm antibodies on sperm
motility analyzed through CASA in fertile and fertile
male subjects.
To determine the impact of ASA in our population and
how it varies in different categories of infertile male
subjects.
To determine whether seminal plasma ASA can be used
as diagnostic tool in evaluating male fertility potential.
9. (n=10) fertile and (n=72) infertile male
subjects.
The infertile patients were subdivided in
to different categories :
(ATZS), (AZS), (OZS), (OAZS), (SA).
Semen volume, sperm count, CASA,
motility parameters were measured
according to WHO criteria.
Antisperm antibody enzyme linked
immunosorbent assay (ELISA) kit.
10.
11. There was non-significant (P>0.05) difference in mean ± SEM
semen volume among fertile and different categories of
infertile male subjects (ATZS, OZS, OAZS, SA).
However azoospermics (AZS) patients showed significant
(P<0.01) decreased in semen volume as compared to fertile
men.
12. a**
0
1
2
3
4
5
6
fertile ATZS AZS OAZS OZS SA
Semenvolume(ml)
Subjects categories
fertile
ATZS
AZS
OAZS
OZS
SA
a**
Figure 1: Mean ± SEM semen volume (ml) of fertile (n=10) and
infertile (n=72) astheno-teratozoospermics (ATZS),
azoospermics (AZS), oligoasthenozoospermics (OAZS)
oligozoospermics (OZS) and sperm agglutination (SA) male
subjects.
13. There was highly significant (P<0.001) decrease in sperm
count OZS, OAZS as compared to fertile male subjects.
While patients with sperm agglutination showed highly
significant (P<0.001) increase in sperm count when
compared with fertile.
There were non significant difference (P>0.05) in mean ±
SEM sperm count (mill/ml) among fertile and ATZS infertile
male subjects.
14. Figure :Mean ± SEM sperm count (mill/ml) of fertile (n=10) and
infertile (n=72) astheno-teratozoospermics (ATZS),
azoospermics (AZS), oligoasthenozoospermics (OAZS),
oligozoospermics (OZS) and sperm agglutination (SA) male
subjects.
0
50
100
150
200
250
Fertile ATZS AZS OAZS OZS S.A
Spermcount(mill/ml)
Subjects categories
Fertile
ATZS
AZS
OAZS
OZS
S.A
15. There was significant (P<0.05) difference in mean ± SEM
sperm motility (%) among fertile and different categories of
infertile male subjects.
16. Mean ± SEM sperm motility (%) in different classes of sperm motility (rapid
progressive motile (%), slow progressive motile (%), and non motile (%) of fertile
(n=10) and infertile (n=72) astheno-teratozoospermics (ATZS), azoospermics (AZS),
oligoasthenozoospermics (OAZS), oligozoospermics (OZS) and sperm
agglutination (SA) male subjects.
0
10
20
30
40
50
60
Fertile ATZS OAZS OZS AZS SA
Spermmotility(%)
Subjects categories
17. Curvilinear velocity (VSL µm/sec)
There was non-significant (P>0.05) difference in mean ± SEM
curvilinear velocity (VSL µm/sec among fertile and different
categories of infertile male subjects.
Straight line velocity (VSL µm/sec)
There was significant (P<0.05) difference in mean ± SEM
straight line velocity (VSL µm/sec) among fertile and
different categories of infertile male subjects.
18. Average path velocity (VAP µm/sec)
There was non significant (P>0.05) difference in mean ± SEM
Average path velocity µm/sec (VAP µm/sec) among fertile
and different categories of infertile male subjects.
Wobble (WOB µm/sec)
There was significant (P<0.05) difference in mean ± SEM
average path WOB among fertile and different categories of
infertile male subjects.
19. Amplitude of lateral head (ALH µm/sec)
There was significant (P<0.05) difference in mean ± SEM
amplitude of lateral head µm/sec (ALH) among fertile and
different categories of infertile male subjects.
