MS Thesis, Md. Alimul Islam, MS, Department of Zoology University of Rajshahi, Rajshahi-6205 Bangladesh.

1. Supervised by
Prof. Dr. Md. Nurul Islam
Crop Protection and Toxicology Lab.
Department of Zoology
University of Rajshahi
Bangladesh
A Thesis
Submitted By
Md. Alimul Islam
MS Student, Department of Zoology
University of Rajshahi
Bangladesh

2. Welcome

3. TITLE
Control potentials of two ornamental indoor
plants Epipremnum aureum (Lindne and Andre)
G. S. Bunting and Aglaonema marantifolium
Schott. against three stored product pests

4. INTRODUCTION
Epipremnum aureum (Money plant)
Test plant 1.
I. About the plant
II. Why we used this plant

5. Aglaonema marantifolium (Chinese evergreen)
INTRODUCTION
Test plant 2.
II. Why we used this plant
I. About the plant

6. Tribolium castaneum (Red flour beetle)
INTRODUCTION
Test agent 1.
II. Why we used this agent
I. About the agent

7. Sitophilus oryzae (Rice weevil)
INTRODUCTION
Test agent 2.
II. Why we used this agent
I. About the agent

8. Callosobruchus chinensis (Pulse beetle)
INTRODUCTION
Test agent 3.
II. Why we used this agent
I. About the agent

9. I. Red flour beetle II. Rice weevil III. Pulse beetle

10. Confused flour by red flour beetle

11. Damaged rice by rice weevil

12. Destructive pulse by pulse beetle

13. AIMS AND OBJECTIVES
I. To trace presence of
pest control potentials
(pesticidal and repellent
activity) those plants
II. To evaluate efficacy
of the promising extracts
against the stored
product pest

14. MATERIALS AND METHODS
I. Collection of plant materials
Money plant Chinese evergreen

15. II. Preparation of plants materials for extraction
MATERIALS AND METHODS
Chopped plant materials Materials after grinding

16. III. Chemical extraction of the collected and prepared materials
MATERIALS AND METHODS
Plant materials in solvent for extraction kept on a shaker

17. III. Chemical extraction of the collected and prepared materials
MATERIALS AND METHODS
Filtration of the extracts followed by labelled vialing

18. EMECEP
Extraction by
petroleum ether
Extraction by
chloroform
Extraction by
methanol
Dust
Plant materials
Extraction of the collected and prepared materials
MATERIALS AND METHODS

19. IV. Collection and Culture of test insects
Collection and culture of T. castaneum
MATERIALS AND METHODS
Mass culture
Prepared food
Adults
Larvae

20. IV. Collection and culture of test insects
Collection and culture of S. oryzae
MATERIALS AND METHODS

21. IV. Collection and culture of test insects
Collection and culture of C. chinensis
MATERIALS AND METHODS

22. V. Dose mortality tests on insects
Dose mortality test on T. castaneum
MATERIALS AND METHODS

23. V. Dose mortality tests on insects
Dose mortality test on S. oryzae
MATERIALS AND METHODS

24. V. Dose mortality tests on insects
Dose mortality test on C. chinensis
MATERIALS AND METHODS

25. VI. Repellent activity tests on insects
Repellency test on T. castaneum
MATERIALS AND METHODS

26. VI. Repellent activity tests on insects
Repellency test on S. oryzae
MATERIALS AND METHODS

27. VI. Repellent activity tests on insects
Repellency test on C. chinensis
MATERIALS AND METHODS

28. FINDINGS
I. Mortality effects on the insects
Test plants Extracts Test agents Activity detected
E. aureum Pet. ether
T. castaneum √
S. oryzae √
C. chinensis √
A. marantifolium
Pet. ether
T. castaneum √
S. oryzae √
CH3OH C. chinensis √

29. FINDINGS
II. Mortality effects on the insects with LD50 values
Test plants Extracts
Test agents
LD50 values (mg/cm2)
E. aureum Pet. ether
6h 12h 18h 24h 30h 36h 42h
T. castaneum 1.40 1.21 1.16 1.10 1.04 0.96 0.94
S. oryzae 1.73 1.66 1.32 1.08 1.00 0.99 0.90
C. chinensis 2.95 2.05 2.01 1.91 1.82 1.72 1.65
A. marantifolium
Pet. ether
T. castaneum 1.34 1.19 1.14 1.12 1.07 1.03 1.02
S. oryzae 1.04 0.91 0.87 0.83 0.80 0.78 0.77
CH3OH C. chinensis 2.34 2.15 1.93 1.86 1.83 1.82 1.81

30. FINDINGS
III. Repellency effects on the insects
Test plants Extracts Test agents Level of Significance
E. aureum
Pet. ether
T. castaneum 5% (p<0.05)
C. chinensis 5% (p<0.05)
CHCl3
T. castaneum 1% (p<0.01)
C. chinensis 5% (p<0.05)
A. marantifolium CHCl3 C. chinensis 5% (p<0.05)

31. OUTCOME
E. aureum- Pet. ether extract and
A. marantifolium- Pet. ether and CH3OH extracts
Mortality
E. aureum- Pet. Ether and CHCl3 extracts
A. marantifolium- CHCl3 extract
Repellency

32. We found the plants were very much promising and
their efficacy on stored grain pests might have future
to be used as a control agent or tool. Being natural in
origin these materials could be biodegradable and
thus safe and thereby could be eco-friendly to the
environment.
OUTCOME

33. ACKNOWLEDGEMENT
The author is grateful to the Chairman, Department of
Zoology, University of Rajshahi for providing laboratory
facilities during the research work.
And also wish to extend his thanks to his supervisor and all of
the honorable teachers of the Department of Zoology,
University of Rajshahi for their kind support and constant
encouragement.

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39. THANNKS!
for being
with ME