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AP Biology Rapid Learning Series - 18

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Plant Function

AP Biology Rapid Learning Series
Wayne Huang, PhD
Andrew Graham, PhD
Elizabeth James, PhD
Casandra Rauser, PhD
Jessica Habashi, PhD
Sara Olson, PhD
Jessica Barnes, PhD

Rapid Learning Center
www.RapidLearningCenter.com/
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1
AP Biology Rapid Learning Series - 18

Learning Objectives
By completing this tutorial, you will learn about:

Plant N t iti
Pl t Nutrition
Plant Hormones
Plant Defense
Plant Reproduction

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Plant Function Concept Map
Plant Growth

Soil

Air

Plant reproduction

Regulated by
Flower structure

Insects
pathogens

Plant Hormones
Seed Development

Auxin

ABAs

Cytokinins
GAS

Ethylene

Phototropism
Growth promotion
Gravitropism
Apical Dormancy
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AP Biology Rapid Learning Series - 18

Plant Nutrition
Plants obtain their nutrients from soil and air.

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Plant Nutritional Sources
The major source of plant
nutrition is fixation of
atmospheric CO2 using
photosynthesis to produce
simple sugar
sugar.

Photosynthesis is the source
of energy to make sugars.

Soil and water are
also sources of
nutrition. They
provide an
anchorage as well as
minerals and
nutrients.
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3
AP Biology Rapid Learning Series - 18

Nutrient Classes
Nonmineral nutrients from
air and water, carbon,
hydrogen and oxygen.
oxygen

Mineral nutrients from soil
include macronutrients
which are required in large
quantities.

Micronutrients are
required in small
quantities.
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Macronutrients
Primary macronutrients
include: nitrogen,
phosphorus and
potassium.

Secondary macronutrients
include: calcium, magnesium
and sulfur.

Potassium

Calcium
Nitrogen

Magnesium

Sulfur

Phosphorus

Secondary
Primary

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AP Biology Rapid Learning Series - 18

7 Micronutrients
There are 7 micronutrients. These
are needed in small amounts.

Copper (Cu)

Iron (Fe)

Zinc (Zn)

Boron (B)
Molybdenum (Mo)

Chloride (Cl)
Manganese (Mn)

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Obtaining Nutrients
Macronutrients are
usually not sufficient in
cultivated soil and need
to be added as fertilizer.

Plants get nutrients
from soil (roots) or air
(leaves and stems).

Natural plant communities
recycle nutrients for use
by other organisms.

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•
•
•
•

Other ways to get nutrients include:
Nitrogen fixation by bacteria.
Carnivorous feeding.
Symbiotic association with fungi.
Parasitic plants.

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5
AP Biology Rapid Learning Series - 18

Transpiration
Transpiration is the evaporation of
excess water from aerial parts of a
plant. This is done by leaves,
stems, flowers and fruits.
Root absorbs minerals
and water from soil
Water and solute are
transported up through
plants via xylem
The driving force is the
evaporation from leaves
via guard cells
This process is called
transpiration
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Transportation of Carbohydrates
Leaves make
y
carbohydrates to
support plant life –
sugar source
Carbohydrates are
transported down via
phloem in vascular
tissue to where they
can b used –sugar
be
d
sink

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AP Biology Rapid Learning Series - 18

Plant Hormones
Plant Growth and Development

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Plant Hormones
Five major types of hormones that control plant growth and
development.

1. Auxin
2. Gibberellins
(GA)
3. Cytokinins
4. Ethylene
5. Abscisic
Acid (ABA)

A hormone is a chemical
messenger between
cells. They signal target
cells to change a
physiological activity.

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AP Biology Rapid Learning Series - 18

Auxin
Auxins are produced by apical
tissues and developing leaves
and they stimulates downward
growth.

Auxins function at the cell
level by stimulating cell
elongation and cell division.

The most important auxin
is indole-3-acetic acid
(IAA).

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Auxin Functions
Auxins are plant hormones that are
Auxin
involved in the coordination of many
growth and behavior processes in
the plant s life cycle.
plant’s
cycle
Auxins are used to promote
the growth of roots as
well as flowering, setting
fruits and preventing
p
premature fruit drop.
p

Auxins act by stimulating or
inhibiting the expression
of specific genes.
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8
AP Biology Rapid Learning Series - 18

Apical Dominance
Apical dominance is
the inhibition of
lateral buds by auxin
from the apical bud.

Dormant
lateral bud

Auxin is broken
down as it moves
down stem, so its
concentration
decreases.

Apical bud removed

Removal of apical
bud releases apical
dominance

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Phototropism
Phototropism: when
illuminated from one
direction, the shoot
proceeds to grow in that
direction.
When illuminated, Auxin
accumulates on the shady
side of a plant
g
This stimulate elongation of
cells from the shady side
and bend the plant to the
light

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9
AP Biology Rapid Learning Series - 18

Gravitropism
Plant growth and its movements
respond to gravity. This is
known as gravitropism.

19/42

When a plant is put into a
horizontal position plant
shoots will bend upward
because auxin accumulates
on the lower part of the stem
called negative gravitropism.

Plant roots will respond by
bending down which is known
as positive gravitropism. This
is because of the accumulation
of starch granules in the root
caps.

Cytokinins – Adenine Derivatives
Cytokinins stimulate plant cell
differentiation. Notice the
difference in these two plants of
treated versus untreated.

•
•
•

•
•

Cytokinin Function
Stimulate cell division
Major source: roots
and apical meritstem
Opposite to auxin:
1)Move up a d; 2)
) o e upward; )
promote growth of
lateral buds
Prevents leaf
senescence
Essential for plant cell
culture.

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10
AP Biology Rapid Learning Series - 18

Gibberellins
Gibberellins promote stem elongation, as
well as in “bolting” which is sudden stem
elongation. It breaks the dormancy of seeds
and stimulates flowering mature plants.
plants

There are more than
30 structurally
related compounds.
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Abscisic Acid
It is a major plant stress
hormone. Abscisic acid is a
plant hormone involved in
adscission (shedding) of plant
y
parts and in bud dormancy.

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Abscisic acid is involved in the
closing of stomata. It inhibits
fruit ripening and encourages
seed dormancy by inhibiting cell
growth.

In winter preparation
abscisic acid is produced in
the terminal buds and slows
growth and protects buds
during the cold.

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11
AP Biology Rapid Learning Series - 18

Ethylene
The only gas form of plant hormone, produced by fruit.

Function
Promote fruit ripening
Stimulates senescence
and abscission in leaves
and fruits
Minor functions:

H2C=CH2

Ethylene

Sprouting of potato buds;
p
g p
;
Seed germination;
Flower formation in some
species

23/42

What Plant Hormone Trigger Growth?
Do you know what plant
hormone is involved in
p
promoting growth?
gg

If you said auxin,
cytokinin and
gibberellins you are
right.

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12
AP Biology Rapid Learning Series - 18

Plant Defense
How plants cope with stress and insects

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Plant Defense
There are two basic
strategies of plant
defense: physical and
chemical barrier.

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Physical barriers against
herbivores include:
• Cuticle: a layer of wax on
the surface of leaves
• Trichomes: extension of
plant cells
• Spines which are modified
leaves.
• Bark

Chemical barriers: secondary metabolites or chemical
toxins that could cause problems for the predator.
These chemicals include isoprenes, phenolic
compounds and alkaloids.

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13
AP Biology Rapid Learning Series - 18

Systemin Response
Plant response to wound: systemin

Synthesis f
S th i of
Systemin

Synthesis f
S th i of
Proteinase inhibitors

Plants under attack

Systemin is a signal molecule for plants to sense the wound and
triggers a series of biochemical reaction to release proteinase
inhibitors which are toxic to insects.

27/42

Plant Defense to Pathogens
Virus
Infection
Cell Death
Hypersensitive
Response (HR)

■ Gene-for-Gene Theory
To every pathogen avirulence (avr) gene, there is a
corresponding R gene (resistance gene) in plant to trigger
HR
HR is commonly followed by a slower response that leads
to systemin acquired resistance (SAR). SAR occurs when a
hormone, which may be salicylic acid, travels from the
infection site to nearby tissues and triggers the expression
of a specific set of genes.
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14
AP Biology Rapid Learning Series - 18

How Does a Plant Respond to Injury?
How do plants
respond to being
wounded?

It mounts a systemin
response involving
signaling from
systemin.

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Plant Reproduction

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AP Biology Rapid Learning Series - 18

Sexual and Asexual Reproduction
Plants can reproduce
asexually and sexually.

Yes, sexual reproduction involves
male gametes (sperms) and female
gametes (eggs) which combine to
create a zygote.

In asexual reproduction
offspring are produced
by mitosis and are
genetically identical to
the parent.

Female structures
house the embryo
during development.
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Life Cycle of a Flowering Plant

Seed production
Seeds

Seed germination

Adult plant
with flowers
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Pea plant

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Seedling

16
AP Biology Rapid Learning Series - 18

Flowers are Reproductive Organs
Stigma

Pedal

Ovary

Carpel
(female organs)

Anther
Filament

Steman
(male organs)

Sepal

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Formation of Gametes
Microspores -->
Pollens

Mother Cell (2N)
Meiosis

Megaspores --> Eggs
Pollen
Mother Cell

1N
2N

Meiosis
Pollen
Grain

Pollen
Grain

Pollen
Grain

Pollen
Grain

1N
Pollen grain in anther

3
megaspores
degenerate, 1
survives
3 times mitosis
then cell dividing
1N egg cell
2N central cell
t l ll
1N egg cell
Embryo sac in ovary

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AP Biology Rapid Learning Series - 18

Pollination
Pollination: Pollen from male organs are transferred to
surface of stigma
Sperms meet megaspores (eggs)
1

Self-pollination
Self-pollination,
same plant but
different flower

2

3

p
Cross-pollination
Different plants

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The link ed image cannot be display ed. The file may hav e been mov ed, renamed, or deleted. Verify that the link points to the correct file and location.

Formation of Zygotes
Pollen tube grows, two sperm cells are delivered into ovary
One sperm fertilize one of the 6 eggs, one fertilizes the central
cell
Fertilized egg develops into 2N zygotes and fertilized central
cell develop into endosperm (3N)
Zygotes attach to parental plant to acquire nutrients which is to
be stored in cotyledons
Ovary
3N
1N

1N
1N

Central cell (2N)

1N

1N

Egg cell (1N)

3N

1N

3N
3N

Endosperm
(3N)
Embryo (2N)

2 sperm nuclei
Pollen tube

Double pollination
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18
AP Biology Rapid Learning Series - 18

Seed Structure
Zygotes grow and differentiate, acquiring and
store food in cotyledons and seed forms.

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How are Seeds Developed?
Double pollination.
Pollination of eggs forms
the embryo.

How does a seed
develop?

Acorn

Pollination of the central cell
forms the endosperm
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19
AP Biology Rapid Learning Series - 18

Question: Review
____ is the evaporation of
excess water from aerial parts
of a plant.
Auxins ti l t
A i stimulate _____
growth.

Transpiration
___________
Downward
D
d
___________

_______ is the inhibition of
lateral buds by auxin from
the apical bud.

___________
Apical dominance

_____ stimulate plant cell
differentiation.

Cytokinins
___________

____ promote stem
elongation and bolting.

___________
Gibberellins

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Learning Summary
Plant defense

Plant nutrients

Natural barrier
Macronutrients
micronutrients

Against wound
Against pathogen

Plant reproduction
Flowers
Zygotes

Plant
Pl t growth and
th d
development
Plant hormones

Seeds
40/42

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20
AP Biology Rapid Learning Series - 18

Congratulations
You have successfully completed the
core tutorial

Plant Function
Rapid Learning Center

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Plant function slides

  • 1. AP Biology Rapid Learning Series - 18 Rapid Learning Center Chemistry :: Biology :: Physics :: Math Rapid Learning Center Presents … p g Teach Yourself AP Biology in 24 Hours *AP is a registered trademark of the College Board, which does not endorse, nor is affiliated in any way with the Rapid Learning courses. Plant Function AP Biology Rapid Learning Series Wayne Huang, PhD Andrew Graham, PhD Elizabeth James, PhD Casandra Rauser, PhD Jessica Habashi, PhD Sara Olson, PhD Jessica Barnes, PhD Rapid Learning Center www.RapidLearningCenter.com/ © Rapid Learning Inc. All rights reserved. © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 1
  • 2. AP Biology Rapid Learning Series - 18 Learning Objectives By completing this tutorial, you will learn about: Plant N t iti Pl t Nutrition Plant Hormones Plant Defense Plant Reproduction 3/42 Plant Function Concept Map Plant Growth Soil Air Plant reproduction Regulated by Flower structure Insects pathogens Plant Hormones Seed Development Auxin ABAs Cytokinins GAS Ethylene Phototropism Growth promotion Gravitropism Apical Dormancy 4/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 2
  • 3. AP Biology Rapid Learning Series - 18 Plant Nutrition Plants obtain their nutrients from soil and air. 5/42 Plant Nutritional Sources The major source of plant nutrition is fixation of atmospheric CO2 using photosynthesis to produce simple sugar sugar. Photosynthesis is the source of energy to make sugars. Soil and water are also sources of nutrition. They provide an anchorage as well as minerals and nutrients. 6/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 3
  • 4. AP Biology Rapid Learning Series - 18 Nutrient Classes Nonmineral nutrients from air and water, carbon, hydrogen and oxygen. oxygen Mineral nutrients from soil include macronutrients which are required in large quantities. Micronutrients are required in small quantities. 7/42 Macronutrients Primary macronutrients include: nitrogen, phosphorus and potassium. Secondary macronutrients include: calcium, magnesium and sulfur. Potassium Calcium Nitrogen Magnesium Sulfur Phosphorus Secondary Primary 8/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 4
  • 5. AP Biology Rapid Learning Series - 18 7 Micronutrients There are 7 micronutrients. These are needed in small amounts. Copper (Cu) Iron (Fe) Zinc (Zn) Boron (B) Molybdenum (Mo) Chloride (Cl) Manganese (Mn) 9/42 Obtaining Nutrients Macronutrients are usually not sufficient in cultivated soil and need to be added as fertilizer. Plants get nutrients from soil (roots) or air (leaves and stems). Natural plant communities recycle nutrients for use by other organisms. 10/42 • • • • Other ways to get nutrients include: Nitrogen fixation by bacteria. Carnivorous feeding. Symbiotic association with fungi. Parasitic plants. © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 5
  • 6. AP Biology Rapid Learning Series - 18 Transpiration Transpiration is the evaporation of excess water from aerial parts of a plant. This is done by leaves, stems, flowers and fruits. Root absorbs minerals and water from soil Water and solute are transported up through plants via xylem The driving force is the evaporation from leaves via guard cells This process is called transpiration 11/42 Transportation of Carbohydrates Leaves make y carbohydrates to support plant life – sugar source Carbohydrates are transported down via phloem in vascular tissue to where they can b used –sugar be d sink 12/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 6
  • 7. AP Biology Rapid Learning Series - 18 Plant Hormones Plant Growth and Development 13/42 Plant Hormones Five major types of hormones that control plant growth and development. 1. Auxin 2. Gibberellins (GA) 3. Cytokinins 4. Ethylene 5. Abscisic Acid (ABA) A hormone is a chemical messenger between cells. They signal target cells to change a physiological activity. 14/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 7
  • 8. AP Biology Rapid Learning Series - 18 Auxin Auxins are produced by apical tissues and developing leaves and they stimulates downward growth. Auxins function at the cell level by stimulating cell elongation and cell division. The most important auxin is indole-3-acetic acid (IAA). 15/42 Auxin Functions Auxins are plant hormones that are Auxin involved in the coordination of many growth and behavior processes in the plant s life cycle. plant’s cycle Auxins are used to promote the growth of roots as well as flowering, setting fruits and preventing p premature fruit drop. p Auxins act by stimulating or inhibiting the expression of specific genes. 16/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 8
  • 9. AP Biology Rapid Learning Series - 18 Apical Dominance Apical dominance is the inhibition of lateral buds by auxin from the apical bud. Dormant lateral bud Auxin is broken down as it moves down stem, so its concentration decreases. Apical bud removed Removal of apical bud releases apical dominance 17/42 Phototropism Phototropism: when illuminated from one direction, the shoot proceeds to grow in that direction. When illuminated, Auxin accumulates on the shady side of a plant g This stimulate elongation of cells from the shady side and bend the plant to the light 18/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 9
  • 10. AP Biology Rapid Learning Series - 18 Gravitropism Plant growth and its movements respond to gravity. This is known as gravitropism. 19/42 When a plant is put into a horizontal position plant shoots will bend upward because auxin accumulates on the lower part of the stem called negative gravitropism. Plant roots will respond by bending down which is known as positive gravitropism. This is because of the accumulation of starch granules in the root caps. Cytokinins – Adenine Derivatives Cytokinins stimulate plant cell differentiation. Notice the difference in these two plants of treated versus untreated. • • • • • Cytokinin Function Stimulate cell division Major source: roots and apical meritstem Opposite to auxin: 1)Move up a d; 2) ) o e upward; ) promote growth of lateral buds Prevents leaf senescence Essential for plant cell culture. 20/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 10
  • 11. AP Biology Rapid Learning Series - 18 Gibberellins Gibberellins promote stem elongation, as well as in “bolting” which is sudden stem elongation. It breaks the dormancy of seeds and stimulates flowering mature plants. plants There are more than 30 structurally related compounds. 21/42 Abscisic Acid It is a major plant stress hormone. Abscisic acid is a plant hormone involved in adscission (shedding) of plant y parts and in bud dormancy. 22/42 Abscisic acid is involved in the closing of stomata. It inhibits fruit ripening and encourages seed dormancy by inhibiting cell growth. In winter preparation abscisic acid is produced in the terminal buds and slows growth and protects buds during the cold. © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 11
  • 12. AP Biology Rapid Learning Series - 18 Ethylene The only gas form of plant hormone, produced by fruit. Function Promote fruit ripening Stimulates senescence and abscission in leaves and fruits Minor functions: H2C=CH2 Ethylene Sprouting of potato buds; p g p ; Seed germination; Flower formation in some species 23/42 What Plant Hormone Trigger Growth? Do you know what plant hormone is involved in p promoting growth? gg If you said auxin, cytokinin and gibberellins you are right. 24/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 12
  • 13. AP Biology Rapid Learning Series - 18 Plant Defense How plants cope with stress and insects 25/42 Plant Defense There are two basic strategies of plant defense: physical and chemical barrier. 26/42 Physical barriers against herbivores include: • Cuticle: a layer of wax on the surface of leaves • Trichomes: extension of plant cells • Spines which are modified leaves. • Bark Chemical barriers: secondary metabolites or chemical toxins that could cause problems for the predator. These chemicals include isoprenes, phenolic compounds and alkaloids. © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 13
  • 14. AP Biology Rapid Learning Series - 18 Systemin Response Plant response to wound: systemin Synthesis f S th i of Systemin Synthesis f S th i of Proteinase inhibitors Plants under attack Systemin is a signal molecule for plants to sense the wound and triggers a series of biochemical reaction to release proteinase inhibitors which are toxic to insects. 27/42 Plant Defense to Pathogens Virus Infection Cell Death Hypersensitive Response (HR) ■ Gene-for-Gene Theory To every pathogen avirulence (avr) gene, there is a corresponding R gene (resistance gene) in plant to trigger HR HR is commonly followed by a slower response that leads to systemin acquired resistance (SAR). SAR occurs when a hormone, which may be salicylic acid, travels from the infection site to nearby tissues and triggers the expression of a specific set of genes. 28/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 14
  • 15. AP Biology Rapid Learning Series - 18 How Does a Plant Respond to Injury? How do plants respond to being wounded? It mounts a systemin response involving signaling from systemin. 29/42 Plant Reproduction 30/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 15
  • 16. AP Biology Rapid Learning Series - 18 Sexual and Asexual Reproduction Plants can reproduce asexually and sexually. Yes, sexual reproduction involves male gametes (sperms) and female gametes (eggs) which combine to create a zygote. In asexual reproduction offspring are produced by mitosis and are genetically identical to the parent. Female structures house the embryo during development. 31/42 Life Cycle of a Flowering Plant Seed production Seeds Seed germination Adult plant with flowers 32/42 Pea plant © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com Seedling 16
  • 17. AP Biology Rapid Learning Series - 18 Flowers are Reproductive Organs Stigma Pedal Ovary Carpel (female organs) Anther Filament Steman (male organs) Sepal 33/42 Formation of Gametes Microspores --> Pollens Mother Cell (2N) Meiosis Megaspores --> Eggs Pollen Mother Cell 1N 2N Meiosis Pollen Grain Pollen Grain Pollen Grain Pollen Grain 1N Pollen grain in anther 3 megaspores degenerate, 1 survives 3 times mitosis then cell dividing 1N egg cell 2N central cell t l ll 1N egg cell Embryo sac in ovary 34/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 17
  • 18. AP Biology Rapid Learning Series - 18 Pollination Pollination: Pollen from male organs are transferred to surface of stigma Sperms meet megaspores (eggs) 1 Self-pollination Self-pollination, same plant but different flower 2 3 p Cross-pollination Different plants 35/42 The link ed image cannot be display ed. The file may hav e been mov ed, renamed, or deleted. Verify that the link points to the correct file and location. Formation of Zygotes Pollen tube grows, two sperm cells are delivered into ovary One sperm fertilize one of the 6 eggs, one fertilizes the central cell Fertilized egg develops into 2N zygotes and fertilized central cell develop into endosperm (3N) Zygotes attach to parental plant to acquire nutrients which is to be stored in cotyledons Ovary 3N 1N 1N 1N Central cell (2N) 1N 1N Egg cell (1N) 3N 1N 3N 3N Endosperm (3N) Embryo (2N) 2 sperm nuclei Pollen tube Double pollination 36/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 18
  • 19. AP Biology Rapid Learning Series - 18 Seed Structure Zygotes grow and differentiate, acquiring and store food in cotyledons and seed forms. 37/42 How are Seeds Developed? Double pollination. Pollination of eggs forms the embryo. How does a seed develop? Acorn Pollination of the central cell forms the endosperm 38/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 19
  • 20. AP Biology Rapid Learning Series - 18 Question: Review ____ is the evaporation of excess water from aerial parts of a plant. Auxins ti l t A i stimulate _____ growth. Transpiration ___________ Downward D d ___________ _______ is the inhibition of lateral buds by auxin from the apical bud. ___________ Apical dominance _____ stimulate plant cell differentiation. Cytokinins ___________ ____ promote stem elongation and bolting. ___________ Gibberellins 39/42 Learning Summary Plant defense Plant nutrients Natural barrier Macronutrients micronutrients Against wound Against pathogen Plant reproduction Flowers Zygotes Plant Pl t growth and th d development Plant hormones Seeds 40/42 © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 20
  • 21. AP Biology Rapid Learning Series - 18 Congratulations You have successfully completed the core tutorial Plant Function Rapid Learning Center 41/42 Rapid Learning Center Chemistry :: Biology :: Physics :: Math What’s N t Wh t’ Next … Step 1: Concepts – Core Tutorial (Just Completed) Step 2: Practice – Interactive Problem Drill Step 3: Recap – Super Review Cheat Sheet Go for it! 42/42 http://www.RapidLearningCenter.com © Rapid Learning Inc. All rights reserved. :: http://www.RapidLearningCenter.com 21