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Gene Inactivation in Green Sulfur Bacterium

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Heather Jordan
Heather Jordan

The document summarizes a study that used natural transformation to create gene knockouts in the green sulfur bacterium Chlorobium tepidum. The study demonstrated that C. tepidum is naturally transformable and can be used to inactivate genes via homologous recombination. Specifically, the researchers inactivated the nifD gene involved in nitrogen fixation to validate the method. Over 30 genes have been inactivated using this approach to study various metabolic pathways in C. tepidum.

TechnologieSanté & Médecine
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Chromosomal Gene Inactivation in the Green Sulfur Bacterium Chlorobium tepidum by Natural Transformation NIELS-ULRIK FRIGAARD* AND DONALD A. BRYANT Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania Journal Article Review Heather Jordan BMMB 507 April 8, 2003
What is Chlorobium tepidum ? ,[object Object],[object Object]
What is Chlorobium tepidum ? ,[object Object],[object Object],[object Object]
What is Chlorobium tepidum ? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What is Chlorobium tepidum ? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Current Research Interests ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genome Highlights ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transformation of C. tepidum ,[object Object]
Transformation of C. tepidum ,[object Object],[object Object]
Transformation of C. tepidum ,[object Object],[object Object],[object Object]
Transformation of C. tepidum ,[object Object],[object Object]
Transformation of C. tepidum ,[object Object],[object Object],[object Object]
Transformation of C. tepidum ,[object Object],[object Object],[object Object],[object Object]
Transformation of C. tepidum ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Nitrogen Fixation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Nitrogen Fixation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Study Objective ,[object Object],[object Object],[object Object],[object Object]
Maps ,[object Object],
Maps ,[object Object],[object Object],
Maps ,[object Object],[object Object],[object Object],
Maps ,[object Object],[object Object],[object Object],[object Object],
Creating a nifD knockout ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Antibiotic Sensitivity ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Optimization of Transformation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],• Gentamicin-resistant transformants ° Transformation Frequency
Optimization of Transformation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],• Gentamicin-resistant transformants ° Transformation Frequency
Optimization of Transformation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],[object Object]
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],{ { {
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],[object Object],{ { {
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],[object Object],[object Object],{ { {
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Effect of Variation in Length of Homologous Flanking DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Various Selection Markers ,[object Object],[object Object],[object Object]
Various Selection Markers ,[object Object],[object Object],[object Object],[object Object]
Various Selection Markers ,[object Object],[object Object],[object Object],[object Object]
Test of Transformants ,[object Object],[object Object],[object Object],[object Object],  
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Guidelines for Routine Gene Inactivation by Natural Transformation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
??Questions??

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Gene Inactivation in Green Sulfur Bacterium

  • 1. Chromosomal Gene Inactivation in the Green Sulfur Bacterium Chlorobium tepidum by Natural Transformation NIELS-ULRIK FRIGAARD* AND DONALD A. BRYANT Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania Journal Article Review Heather Jordan BMMB 507 April 8, 2003
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