integrated approach to tree domestication and conservation of genetic resources
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integrated approach to tree domestication and conservation of genetic resources
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integrated approach to tree domestication and conservation of genetic resources
- 1. INTEGRATED APPROACH TO TREE DOMESTICATION AND CONSERVATION OF GENETIC RESOURCES DANIEL OFORI (GRP1 / EA) ICRAF 23 RD AUGUST 2011
- 9. THE IROKO (Mvule) STORY Milicia sp . (M. Excelsa & M. Regia) Considered as the most useful species in the family - MORACEAE
- 11. Milicia-Phytolyma lata problem Extraction=172,983 m 3 yr -1 ; regeneration = 28,650 m 3 yr -1 ; Deficit =144,333 m 3 yr -1
- 12. Impact of Pest on Plant Growth
- 13. Mean growth rate of protected and unprotected Milicia after 18 months
- 14. Productivity loss of unprotected Milicia after 18 months
- 15. Screening for pest resistance 133 accessions from 4 Ghanaian provenances
- 16. Seedling response to pest attack
- 17. Selection of resistant individuals R S S R S Trees marked ‘R’ are resistant Trees marked ‘S’ are susceptible
- 19. Conservation of desirable genotypes Stem and root cuttings Rooted stem cuttings Root cuttings with shoots
- 20. Grafting Collection of scion Grafted plant
- 21. Air layering Ready for planting Girdled branch With ball of rooting medium
- 22. In vitro tissue culture Shoots grown from axillary buds Ex-situ conservation Serial propagation
- 23. Genetic gains from clonal trial at 12 months after establishment
- 25. DNA fingerprinting of Milicia M . excelsa M . regia M. excelsa M. regia
- 27. Analysis of variance components revealed most of the total variation was among populations (65%) SV df SS MSS Variance component % total variation Among pop 4 96.45 24.12 5.32 65.26 Within pop 15 42.50 2.83 2.83 34.75
- 28. Genetic distance among 5 populations Population ID GD GM GW CD GM 0.51 GW 0.71 0.46 CD 0.28 0.72 0.94 SW 0.62 0.43 0.31 0.88
- 32. Cytotype frequency (%) and number of cytotypes in each population. Cytotype 2 is M. regia, and cytotypes 1 and 3 are M. excelsa . Provenances Cytotype1 (Ccmp2-M267) Cytotype2 (Ccmp2-M275) Cytotype3 (Ccmp2-M283) No. of cytotypes in population GD 0 23 77 2 GM 9.1 90.9 0 2 GW 0 100 0 1 CD 0 0 100 1 SW 0 100 0 1
- 34. Phylogenetic analysis using blast (NCBI)
- 38. Cedrela seed orchard management
- 41. Quality germplasm = GRP1 CRP7 Climate change And agric GRP 6 Multifunctional Landscape (envt. Services) GRP4 Land health and restoration GRP3 Tree product marketing GRP2 on farm productivity CRP4 Agric nutrition and health GRP5 Climate change Partners CRP3. sustainable Production syt (Food Security) CRP5 Water, soil & ecosystems CRP1 Integrated agric. systems CRP6 Forests, trees and AF
- 45. Thank you very much Asanteni sana