1. SEMINAR
ANKUSH MEENA
B100974CH
DEPATMENT OF CHEMICAL ENGINEERING
NATIONAL INSTITUTE OF TECHNOLOGY CALICUT
12/03/2014

2.  The Product recovery operation are also known as
downstream processing steps.
 Product recovery in bioprocessing plays a vital role.

3.  Downstream- ‘After the fermentation process’.
 Primary ‘unit operations’ of Downstream
Processing
 Cell recovery /removal
 Dewatering

4.  Secondary ‘unit operations’
 Protein purification
 Adsorption chromatography
 Protein processing
 Immobilisation
 Prilling
 Protein packaging
 Sterilisation etc

5. The cell disruption techniques may be classified as
• Physical method
• Chemical method

6. The following unit operations are used for the separation
of solids :
• Filtration
• Sedimentation
• Centrifugation
• Foam Separation
• Precipitation

7. System Type With phase
change
Without phase
change
Solid-liquid Soluble Drying,
Evaporation,
Adsorption
Ultrafiltration,
Reverse osmosis
Insoluble Drying Filtration,
Sedimentation ,
Liquid-liquid Soluble Distillation Chromatography
Insoluble Extraction Centrifugation
Solid-solid immiscible Selective
Solubilisation
Air Separation
Screening
sievingMiscible Cell disruption
Liquid-liquid-solid Miscible
Immiscible
Adsorption Centrifugation
Sedimentation
Decanting

8.  It is based on the differences in the surface activity.
 It is chemical process of removing a component of a
liquid mixture using a bubbled gas and a surfactant to
collect the material.
 Wastewater treatment systems often use foam
separation steps to remove solids from the water
stream.

10.  It is also a very useful method for product recovery by
adding the precipitation agent .
 The compounds from the corresponding salts or the
suspending solids which would settle down to the
bottom.
 This method is extensively used for product recovery
of protein isolates or protein concentrates from oil
seeds

11.  Filtration is a physical or mechanical process to
separate solids particle from fluids.
 Filtration is a technique used for two main purposes.
 The first is to remove solid impurities from a liquid.
 The second is to collect a desired solid from the
solution.
 It is a very effective and convenient method of
separating the suspending solids from the slurries .
 The fluid that pass through is called a filtrate.

13.  Filtration is used to separate particles and fluid in a
suspension, where the fluid can be a liquid, a gas or
a supercritical fluid.
 Filtration, as a physical operation is very important in
chemistry for the separation of materials of different
chemical composition.
 Filtration is also important and widely used as one of
the unit operations of chemical engineering.

14.  pressure drop
Viscosity of filtrate
Area of filtering surface
Resistance of filtrate cake
Resistance of filtrate surface
Properties of slurry

15. Filters are divided into three main categories
1. Cake filters
2. Clarifying filters
3. Cross-flow filters

16.  Slurries with high solid concentration are operated in
cake filters.
 Very dilute liquids are passed through the clarifying
filters.
 The cross-flow filters are used for concentrating the
solutions by using filtration technique.

18. Thank you