The document describes how to make a natural plant growth formula by combining fermented plant extract and fish hydrolysate, which provides nitrogen and growth-promoting enzymes and hormones to promote plant growth. The fermented plant extract is made by combining green plant materials like leaves with sugar and allowing it to ferment for 7-15 days, then mixing it in a 1:1 ratio with fish hydrolysate high in nitrogen to create a liquid fertilizer and plant tonic. Proper application and dosage instructions are provided to maximize the growth benefits for plants.

1. Biofertilizer Technologies
For Sustainable Agriculture
and Its other Beneficial
Usage
PedroMS,BIOTECH-UPLB,
26Nov2015
Mannix S. Pedro
National Institute of Molecular Biology & Biotechnology (BIOTECH)
UP Los Baños
DA-Bureau of Agricultural Research In-House Seminar Series
Ground Floor, RDMIC Building, Elliptical Road, corner Visayas Avenue
Diliman, Quezon City
20 July 2017

6. Production Imports Supply
1990 934,346 1,118,408 2,052,754
1991 1,007,755 1,102,155 2,109,910
1992 930,144 1,163,288 2,093,432
1993 1,035,431 1,099,286 2,134,717
1994 1,098,877 1,168,315 2,267,192
1995 1,389,770 1,237,576 2,627,346
1996 1,607,437 1,202,158 2,809,595
1997 1,321,900 1,246,105 2,568,005
1998 1,181,272 787,355 1,968,627
1999 1,167,707 1,222,352 2,390,059
2000 1,068,887 1,332,483 2,401,370
Production Imports Supply
2001 1,202,397 1,030,118 2,232,515
2002 1,329,469 906,736 2,236,205
2003 1,059,315 1,640,386 2,699,701
2004 989,829 1,895,139 2,884,968
2005 877,098 1,615,107 2,492,205
2006 778,458 1,438,478 2,216,936
2007 747,884 1,683,393 2,431,277
2008 287,105 1,512,245 1,799,350
2009 196,095 1,733,728 1,929,823
2010 182,549 1,970,489 2,153,038
2011 614,499 1,610,308 2,224,807
2012 981,925 1,295,521 2,277,446
2013P 335,827 872,870 1,208,697
2014P 388,720 2,321,904 2710624
Fertilizer: Supply and Disposition by Year and Item
Supply consists of local production and imports.
Source: Fertilizer and Pesticides Authority/PSA, 2017
Latest update:
2014-12-17 09:00
P - Preliminary
Unit: metric tons

7. BIOTECH
University of the Philippines
Los Baños
The energy crisis in the early 70’s prompted BIOTECH to look for an
alternative or supplement of chemical fertilizers as one of its major thrusts

8. BIOTECH-UPLB
(Biotechnology for Agriculture and Forestry Program)
Development of Microbial fertilizers/inoculants
• role of microorganisms as a possible tool to partially
solve the problems on the demand/supply of fertilizers
was looked into
• vigorous bioprospecting activities were conducted from
1980 – 1990 to harness microorganisms with high N2-
fixing ability, mycorrhizal action, bio-control potential,
and etc.

9. - to develop and apply biotechnology
strategies for improved crop
productivity and sustainability
Goal

10. http://greencrosshydroponics.com/wp-content/uploads/2014/02/nutrients-plants-elements.jpg
What plant needs to grow......
How can microbes help plant to grow???

11. Biological N2-fixation in agricultural and terrestrial natural systems
(Herridge et al. 2008)

12. Key biological processes associated with microbe mediated plant growth promotion through
mechanisms that may either directly or indirectly increase the availability of soil P or
enhance a plant’s capacity to acquire P from soil
Plantphysiol 2011;156:989-996

13. Worldwide, lightning = fixes less than 5 to
10 Tg N/year (1 Teragram, [TG] = 1 M
metric ton)
Microbes are the major natural
suppliers of new biologically
available nitrogen. Before the
widespread planting of legume
crops, terrestrial organisms
probably fixed between 90 and 140
Tg of nitrogen per year.
Peter, et al. 1997
IssuesinEcology

14. http://www.embrapa.br

15. Utilization of Plant-Microbe Interaction
Source: www.biosci.ohio-state.edu

16. Biofertilizer technologies......
farm inputs enriched with beneficial
microorganisms that are active
mediators in the cycling of nutrients
involve in the natural conversions of
nitrogen, phosphorus, potassium,
carbon and others

17. Nitrogen =
Bio-N
Nitroplus
Phosphorus =
Brown Magic
VAMRI
Mykovam
Mykogrow
Mykoplus
PSB
Hormones =
Biogroe
Endophytes
Biofertilizer Products
OM=
Composting
BioFix/BioQuick
Others:
developing products
(FPJ, IMO, MicroMix
Nutrio, Proplant, Acticon,
Hi-Yeast)

18. NITROPLUS
Nitrogen supplement
for legumes for
peanut,sitao,soybean,
and mungbean
BIOGROE
Plant Growth Promoter
of vegetables and
ornamentals
VAM ROOT INOCULANT
Promotes growth of
agricultural and
horticultural crops,trees
and ornamentals
BROWN MAGIC
Effective growth enhancer
and biocontrol of root
diseases of various orchid
species.
Microbial Fertilizers

19. BIO-QUICK
Composting inocula
containing the fungus
Trichoderma sp.
BIO-GREEN
Bio-organic fertilizer
(BOF) derived from farm
and industrial wastes
MYCOGROE-Tree
vitamin to enhance
growth and development
of forest trees and
commercial plantation.
BIO-FIX
Enrichment inoculant
enhancing the nutritive
value and effectivity of
BOF
BIO N
Microbial-based fertilizer
that supplies nitrogen and
enhances growth of
rice,corn, and vegetables
MYKOVAM
Soil-based biofertilizer for
fruit trees, agricultural
crops,reforestation
species, and ornamentals
Microbial Fertilizers

20. Powder inoculants that
contains species of
nitrogen-fixing
bacteria
Bio-N
Replaces 30-50% of N
requirement of rice and
corn
contains species of nitrogen-fixing bacteria that was isolated
from the roots of Saccharum spontaneum L.

21. BIO-N FOR RICE
W/o BIO-N With BIO-N
W/o BIO-N With BIO-N

22. Effect of BIO-N inoculation on the yield of rice cv. RC 18
as affected by chemical fertilizer
(Cabuyao, Laguna, DS 2007 )
TREATMENT FERTILIZER YIELD
(kg./16
m2
Farmer’s Practice 2 bags 14-14-14, 1 bag 46-0-0 3.56 b
Farmer’s Practice +
BIO-N
2 bag 14-14-14, 1 bag 46-0-0 +
BIO-N
7.00 a
½ Recommended
Rate + BIO-N
1 bag 14-14-14, ½ bag 46-0-0 +
BIO-N
6.66 a

23. NitroPlus
Nitrogen-fixing
inoculant for legumes
29-124% increase in
yield of legumes

24. Average % Increase
in Yield Due to NITROPLUS

25. NitroPlus Control
Effect of NitroPlus on Soybean
Planted in Tarlac
NitroPlus Control

26. Plant growth-
promoting rhizobacteria
(PGPR)
BioGroe
Produce:
Solubilize phosphorus
IAA, cytokinins,
gibberellins
Siderophore
ACC deaminase
Produce:
IAA, cytokinins,
gibberellins

27. Early seedling growth of BioGroe-inoculated
ampalaya (cv. Jade Dragon) two weeks after sowing.
Bio-Grow
Ampalaya
BioGroe

28. Sampaguita
Control C BioGroe
Effect of BioGroe on the propagation of
sampaguita at 4 weeks after planting.
Control C BioGroe

29. Sampaguita
Control C BioGroe
Effect of BioGroe on the propagation of
sampaguita at 4 weeks after planting.

30. VAM Root inoculant (VAMRI)
Mycorrhizal Root Inoculant forAgricultural
Crops, Ornamentals and Fruit Crops
•VAM fungus either Glomus
mosseae or Glomus fasciculatum
•Enhance absorption of water and
nutrients particularly phosphorus;
also serves as biocontrol agent of
soil-borne diseases of different
crops.

31. Sugarcane
VAMRI WithoutVAMRI
Sugarcane with VAM produce shoots earlier than the
uninoculated control

32. Eggplant

33. Uninoculated
60g (complete)
VAM Root Inoculant
30g (complete)
VAM Root Inoculant
60g (complete)More fruits were produced by
papaya with VAM Root Inoculant

34. Fruit yield at first picking
VAM at transplanting
Without VAM
VAM at sowing
Yield at first picking
Cv Diamante max

35. Mycorrhizal inoculant containing
spores, infected roots and other
infective propagules of
endomycorrhizal fungi
Replaces about 60-85% of the
plants’ chemical fertilizer
requirement
For almost all plants except
orchids and crucifers
Biological control against root
pathogens
MYKOVAM

36. With Mykovam
Lanzones of Dr. Alexis de Manuel in Cotabato after
applying Mykovam

37. Mr. Ben Rara proudly showing his durian and langka trees
in Bukidnon with numerous fruits after applying Mykovam,
previously with very few fruits.

38. Mycorrhizal inoculant with
spores of ectomycorrhizal
fungi collected under forest
plantations during rainy
season
Replaces about 60-85% of
the plants’ chemical fertilizer
requirement
For trees: Eucalyptus,
Acacia, Casuarina, Alnus,
Dipterocarps and Pines
MYCOGROE TABLETS

39. CONTROL MYCOGROE
CONTROL MYCOGROE
CONTROLMYCOGROE

40. Brown Magic
Mycorrhizal fungal
inoculant for orchids
Induces early flowering
Enhances production of
more suckers & longer
spikes

41. Brown magic without Brown magic

42. Vanda Inoculated with Brown Magic

43. Growth response of Cattleya and
Dendrobium plants to Brown Magic

44. BioQuick, BioFix
and BioGreen
 Microbial inoculants for the bioconversion of crop
residues and agro-industrial by-products into
biofertilizers

45. BioQuick – fungal inoculant for
effective decomposition of
household and agro-industrial
waste
BioFix – enrichment inocula
containing an appropriate strain of
nitrogen-fixing bacteria.
BioGreen – processed inoculated
compost or bioorganic fertilizer.

46. Control Chemical
Fertilizer
BioGreen
BioGreen on Corn in Terms of
Marketable Yield

47. Can replace 50-100% of the plant’s chemical
fertilizer requirement depending on fertility
status of the soil
Thus, farmers may have increased savings/income
They impart a degree of plant resistance or
tolerance to many stressants including soil-borne
pathogens, drought, pollutants, etc.
Contribute to the maintenance of ecological
balance
Impacts/Advantages of Biofertilizers

48. Capacity building activities
onbiofertilizer technologies

49. Region II:
Isabela
Nueva Vizcaya
Region IV:
Laguna
Batangas
Approach: Participatory Rural Appraisal
(PRA) activities with the assistance of
Municipal Agricultural Officers was
conducted
Researchers
Farmers

51. Comparative growth performance of corn as affected by different
treatments such as ½ recommended rate of chemical fertilizer (RRC) and
biofertilizer (VAMRI or BIOGREEN)
(Nueva Vizcaya)

53. Participatory Rural Appraisal in Palawan

54. Farmer’s Field Day, Workshop and Participatory RuralAppraisal
BIOTECH, UPLB, January 12, 2012

57. Carcar, Cebu

59. Thank you!

60. Fermented plant Extract and Fish Hydrolysate

61. This is the natural farming plant growth formula.
The formula is effective for the Nitrogen it supplies, but more importantly for
the growth promoting enzymes and hormones it contains.
Chlorophyll in leaves is not broken down in oil or in water.
They require weak alcohol in order to be broken down.

62. Fermentation produces some alcohol as a byproduct, which breaks down leaves and
releases those enzymes and hormones.
How to Make:
This is a combination of Fish Hydrolysate and fermented plant extract.
Fish Hydrolysate is used because it’s high in Nitrogen, a principal element needed by
growing plants.

65. Rich in many vitamins, minerals and oil.
The plant extracts provide the growth hormones along with other essential macro- and micro-nutrients.
Formula: 1:1 fermented plant material and fish hydrolysate.
Instruction for fermented plan extract:
Look or find a fast-growing plant in your area. (It must be a green-color plant, fresh juicy succulent leaves are
the best one.)
Collect a bunch of the growing tips of the plant.

66. The green leaves give you Nitrogen, the tips give more of the growth hormones. Do not wash them
for you may wash off those microbes. We want the photosynthetic bacteria that naturally occur on
the leaf surface of these fast-growing plant. ( Phyllosp- here microbes).
Weigh the amount of material you’ve collected and add roughly half that weight in sugar.
Put in a clay jar or plastic container.
If fermenting a large batch, put a large rock on top of the material to push it down into the bottom
of the container.

67. After 5 hours, remove the rock, and cover the container with a newspaper/cheese cloth/ and
secure with string or rubber-band.
If fermenting a smaller batch, you can add water. Add at least enough water to cover the material
but if you want to add more no problem.
Keep container out of direct sunlight. Solution will be fermented in approximately 7-15 days.
(depending on the temperature).
After, drain the liquid and put in plastic bottle, leaving 1/3 empty so organisms can breathe.

68. Don’t Tighten lid for at least 2 weeks or so. Wait till tiny bubble disappear and then close the container tightly.
If you observe un-dissolved sugar in the bottom it means fermentation did not go to completion. Add a little
water to reactivate and leave lid off for a few days.
Fermented Plant Extract Procedure:
Mix 1tbsp. or 4tbsp/ gallon.
Apply as foliar spray in the morning or evening when temp is lower. During the middle of the day when sunlight
is highest, the plant stomata are closed(to preserve moisture), thus the intake of our foliar

69. Spray becomes difficult and less effective.
Plant material can be used as animal feed or compost.
Extract should have a sweet, sour and even alcoholic smell and taste.
HOW TO USE COMPLETE GROW FORMULA?
Mix 1 tbsp./gallon.
Apply once per week

70. Apply as Foliar spray in morning/evening
Apply as soil drench anytime
Mix with BIM for enhanced effectiveness.