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A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
Media and biochemical tests
Media and biochemical tests
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Biochemical test of bacteria
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Biochemical tests. 1-IMViC tests. 2-TSI test. = KIA test
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L11 - Medical Microbiology Laboratory (Biochemical tests - II)
Medical Microbiology Laboratory (biochemical tests - ii)
Medical Microbiology Laboratory (biochemical tests - ii)
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Results of the MR and VP tests need to be used in conjunction with other biochemical tests to differentiate genus and species within the Enterobacteriaceae. Most members of the family Enterobacteriaceae give either a positive MR test or a positive VP test. Read the VP test at 48 hours. Increased incubation may produce acid conditions in the broth that will interfere with the readings of the results.
DEMONSTRATION OF METHYL RED AND VOGES-PROSKAUER TEST
DEMONSTRATION OF METHYL RED AND VOGES-PROSKAUER TEST
Anamika Rana
The carbohydrate fermentation test is used to determine whether a bacterium can utilize a certain carbohydrate or not. It tests for the presence of acid and/gas produced from the fermentation of a single carbohydrate.
Carbohydrate fermentation test 1
Carbohydrate fermentation test 1
Univ. of Tripoli
Recommandé
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
Media and biochemical tests
Media and biochemical tests
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Test Review
mynamesjohnwayne
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Biochemical test of bacteria
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Biochemical tests
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L11 - Medical Microbiology Laboratory (Biochemical tests - II)
Medical Microbiology Laboratory (biochemical tests - ii)
Medical Microbiology Laboratory (biochemical tests - ii)
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Results of the MR and VP tests need to be used in conjunction with other biochemical tests to differentiate genus and species within the Enterobacteriaceae. Most members of the family Enterobacteriaceae give either a positive MR test or a positive VP test. Read the VP test at 48 hours. Increased incubation may produce acid conditions in the broth that will interfere with the readings of the results.
DEMONSTRATION OF METHYL RED AND VOGES-PROSKAUER TEST
DEMONSTRATION OF METHYL RED AND VOGES-PROSKAUER TEST
Anamika Rana
The carbohydrate fermentation test is used to determine whether a bacterium can utilize a certain carbohydrate or not. It tests for the presence of acid and/gas produced from the fermentation of a single carbohydrate.
Carbohydrate fermentation test 1
Carbohydrate fermentation test 1
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Combined presentation on:- 1. Sugar fermentation tests 2. Cetrimide agar 3. Hugh Leifson medium Covering principle, preparation of the medium, test protocol, result interpretations, test conclusions.
Sugar fermentation tests, Cetrimide agar medium, Hugh Leifson medium
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Details of all biochemical test used in clinical microbiology and the source of presentation is greatly acknowledged
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INDOLE TEST UREASE TEST CITRATE TEST METHYL RED(MR) TEST VOGES – PROSKAUER(VP) TEST TRIPLE SUGAR IRON(TSI) TEST OXIDASE TEST CATALASE TEST CATALASE TEST-Principle:- This test demonstrates presence of catalase enzyme. This enzyme catalyses the release of O2 from H2O2. catalase 2H2O2 H2O + increase O2 Reagents:- 1) 3% H2O2. 2) 24 hrs cultured organisms Procedure:- With sterile wooden stick transfer culture organisms to test tube containing 3% H2O2 and observe for production of effervescence. It can also be tested directly on growth plate. Positive Control: Staphylococci. Negative Control: Streptococci. False positive reactions: If culture medium contains catalase enzyme e.g., blood agar, chocolate agar. If iron wire loop is used
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Methyl Red (MR) and Voges-Proskauer (VP) Test principle, Method, Interpretation & QC #MR & VP Mallu Medicos Lounge As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks.. Our Partner Channel Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0 Qualification Maneesha M Joseph MSc MLT (Microbiology) Assistant Professor Baby memorial college of allied Health science Kozhikode #Methyl Red Test #Voges-Proskauer Test #MRVP Procedure #MRVP Tests #MR-VP Tests #VP (Voges Proskaeur) Test #MR/VP Test #Methyl red (MR) and Voges-Proskauer (VP) #Methyl red test in Microbiology #Medical #Microbiology #MR/VP Test malayalam lecturer #Mallu Medicos Lounge #MalluMedicosLounge #MLT #Channel introduction #HealthAndVoyage
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Details of all biochemical test used in clinical microbiology and the source of presentation is greatly acknowledged
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Biochemical tests are based on reactions that takes place in various living rganisms. In microbiology these are useful for identification of various microorganisms like identification and differentiation of various bacterial species. IMViC test is a group of test that are used to differentiate between Escheritia and Enterobacter species.
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Biochemical test
shahriarshamim2
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Combined presentation on:- 1. Sugar fermentation tests 2. Cetrimide agar 3. Hugh Leifson medium Covering principle, preparation of the medium, test protocol, result interpretations, test conclusions.
Sugar fermentation tests, Cetrimide agar medium, Hugh Leifson medium
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Shivam kumar Sriwas
Practical microbiology dr.nada khazal k. hendi
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عليے المرزوكيے
Details of all biochemical test used in clinical microbiology and the source of presentation is greatly acknowledged
Biochemical tests (2nd part)
Biochemical tests (2nd part)
Aman Ullah
Lab 5 Biochemical test in Microbiology lab
5th lab 1st part biochemical tests (1)
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INDOLE TEST UREASE TEST CITRATE TEST METHYL RED(MR) TEST VOGES – PROSKAUER(VP) TEST TRIPLE SUGAR IRON(TSI) TEST OXIDASE TEST CATALASE TEST CATALASE TEST-Principle:- This test demonstrates presence of catalase enzyme. This enzyme catalyses the release of O2 from H2O2. catalase 2H2O2 H2O + increase O2 Reagents:- 1) 3% H2O2. 2) 24 hrs cultured organisms Procedure:- With sterile wooden stick transfer culture organisms to test tube containing 3% H2O2 and observe for production of effervescence. It can also be tested directly on growth plate. Positive Control: Staphylococci. Negative Control: Streptococci. False positive reactions: If culture medium contains catalase enzyme e.g., blood agar, chocolate agar. If iron wire loop is used
Biochemical reactions new
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Methyl Red (MR) and Voges-Proskauer (VP) Test principle, Method, Interpretation & QC #MR & VP Mallu Medicos Lounge As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks.. Our Partner Channel Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0 Qualification Maneesha M Joseph MSc MLT (Microbiology) Assistant Professor Baby memorial college of allied Health science Kozhikode #Methyl Red Test #Voges-Proskauer Test #MRVP Procedure #MRVP Tests #MR-VP Tests #VP (Voges Proskaeur) Test #MR/VP Test #Methyl red (MR) and Voges-Proskauer (VP) #Methyl red test in Microbiology #Medical #Microbiology #MR/VP Test malayalam lecturer #Mallu Medicos Lounge #MalluMedicosLounge #MLT #Channel introduction #HealthAndVoyage
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Details of all biochemical test used in clinical microbiology and the source of presentation is greatly acknowledged
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Aman Ullah
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Indole test
Indole test
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PPT
Bacterial identification
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Dr.saumya singh Dr.D.Y.Patil P.G.3rd yr
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Biochemical tests are based on reactions that takes place in various living rganisms. In microbiology these are useful for identification of various microorganisms like identification and differentiation of various bacterial species. IMViC test is a group of test that are used to differentiate between Escheritia and Enterobacter species.
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Biochemical analysis of unknown bacteria Aerotolerance Test Fluid Thioglycollate broth (FTB) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar. The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to water. Methylene blue is an indicator that is colorless in an anaerobic environment and greenish-blue in the presence of oxygen. The agar helps retard oxygen diffusion and helps maintain the stratification of organisms growing in different layers of the broth. Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Obligate aerobes will only grow in this oxygen-rich top layer. On another hand, obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones Reactions typically take up to 1-2 days to develop at 37⁰C Media is inoculated using an inoculating loop (A) Escherichia coli and (C) Staphylococcus aureus: both are Facultative Anaerobe, grows both aerobically and anaerobically and growth is seen throughout the tube. Some are capable of growth respiring with oxygen and anaerobically by fermentation. (B) Clostridium botulinum: Obligate Anaerobe: can not grow in the presence of oxygen, growth is seen approximately 1/4 to 1/2 of the way from the top of the tube. (D) Neisseria sicca: Microaerophile, requires oxygen but at concentrations below atmosphere, grows just below the surface of the media but not at the top. (E) Pseudomonas aeruginosa: Obligate Aerobe: oxygen is required for growth and grows at the top of the tube only. The Organism will “settle” and sink into the media if grown longer than 24 hrs. Aerotolerance Test Phenol red test Phenol red broth is a differential test medium prepared as a base to which a carbohydrate such as sucrose, lactose, dextrose or glucose is added. Included in the base medium are peptone and the pH indicator is phenol red. Phenol red is yellow below pH 6.8, pink to magenta above pH 7.4, and red in between. During preparation, the pH is adjusted to approximately 7.3 so it appears red. Deamination of peptone amino acids produces ammonia which rises the pH and turns the broth pink. An inverted Durham tube is added to each tube as an indicator of gas production. Gas production, also from fermentation, is indicated by a bubble or pocket in the Durham tube where the broth has been displaced. Acid production from fermentation of the carbohydrate lowers the pH below the neutral range of the indicator and turns the medium yellow. Deamination of peptone amino acids produces ammonia which rises the pH and ...
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List of biochemical tests
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Laboratory Biochemical Assays Mcb1010 L
1.
Laboratory Biochemical Assays
A Quick & Concise Review
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
SA on MSA
18.
19.
20.
SP on Blood
Agar
21.
22.
23.
EA & EC
on MacConkey Agar
24.
25.
26.
EA vs. EC
on EMB Agar
27.
28.
29.
Starch Hydrolysis
30.
31.
32.
33.
34.
Carbohydrate Fermentation
35.
36.
Triple Sugar Iron
(TSI)
37.
38.
39.
40.
Indole
41.
42.
43.
44.
45.
MRVP Test
46.
47.
48.
Citrate Utilization
49.
50.
51.
Litmus Milk
52.
Litmus Milk
53.
54.
55.
Nitrate Test
56.
57.
58.
Catalase
59.
60.
61.
Oxidase, at the
bottom
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