You have ligated the cDNA extracted with EcoRI (A), and inserted it into pBIT4 (B), digested with the same restriction enzyme. You have transformed the bacteria and purified the plasmid DNA from three of the antibiotic resitstant clones. You have finally performed the restriction analysis of the purified plasmids, and obtained the results shown in picture C. Answer the following questions: - Which restriction enzyme(s) has(have) been used for the restriciton analysis of the .

1. You have ligated the cDNA extracted with EcoRI (A), and inserted it into pBIT4 (B), digested
with the same restriction enzyme. You have transformed the bacteria and purified the plasmid
DNA from three of the antibiotic resitstant clones. You have finally performed the restriction
analysis of the purified plasmids, and obtained the results shown in picture C. Answer the
following questions: - Which restriction enzyme(s) has(have) been used for the restriciton
analysis of the