1. Am I Surface Water Scientist ?
 Specific to Hydrology, water shed,
pollution but not limnology
 Skill towards Environmental
Innovations & contribution to this
emerging science
 Water quality to aquatic life,
agricultural use and recreation &
aesthetic purpose

2. Aerobic Submerged
Attached Growth
Bioreactor: A Suitable
Device for Intermediary
Wastewater Treatment

3. Specific Objectives:
 To assess the organic matter degradation
efficiency under different solids and
salinity proportions in wastewater.
 To evaluate ammonia nitrogen removal
capability under submerged condition of
bioreactor.
 To find out relation between bacterial
intensity and the degree of waste removal.

4. Experimental setup for the different
particle size experiments

5. Experimental setup for the material
comparison experiments

6. : Initial conditions of MCE (stone,
glass & tire) experiment
Experiment-1 Experiment-2 Experiment-3
COD (mg/L) 2275 2190 4465
BOD (mg/L) 330 760 1506
NH3-N (mg/L) 302 1000 1730
Packed bed depth (cm) 75 90 90
Bacterial Density (cfu/ml) 2.3 * 104 19.2 * 104 300 * 104
Total solids (mg/L) 2400 10400 15200
Total suspended solids (mg/L) 26 200 985
Electrical Conductivity 6060 9500 13500
(µS/m)
Hydraulic loading (m3 m-2 d-1) 2.0 4.0 4.0

7. Measuring parameters and instruments
Measured parameters Measuring instruments
Dissolved oxygen DO meter of model OM-51
Chemical oxygen COD vial and Lambda 20 Spectrometer
demand
Ammonia-nitrogen NH3-N electrode (5002-10C) with multi-meter
model D-53
Total dissolved solid Oven
Total Suspended solid Vacuum extractor and Oven
Electrical conductivity Conductivity meter of model ES-51
pH pH electrode (6066-10C) with multi-meter
Biomass weight Electronic balance
Bio-film thickness Digital caliper
Air flow supply Aerator
Wastewater flow supply Small submergible pump

8. Sample of prepared agar for
culturing bacteria

9. CFU sample collection

10. Bio-oxidation of organic matters in recirculation process

12. Nitrification trends in recirculation process

13. Bacterial performance in recirculation process

16. Overall performance in Third Cycle experiments
COD% BOD% NH3-N% HC% TS% CFU 104 EC
Initial
value 4465 1506 1730 150 15200 300 13500
Mixed tire 53.95 97.17 95.53 47.02 15.30
Large tire 52.86 98.41 85.01 31.90 17.43
Medium
tire 56.67 97.79 79.17 29.37 18.91
Small tire 55.04 99.12 90.34 40.87 15.63
Crumb tire 52.31 98.50 75.44 100.00 13.65
Mean 54.63 98.12 87.51 37.29 16.82
STDEV 1.6 0.8 7.0 8.2 1.7
Stone 55.3 97.6 96.8 22.4
Glass 55.0 98.7 93.1 22.6
Tire 55.6 98.6 81.3 23.8
Mean 55.31 98.29 90.39 22.97

25. Conclusion
 Aerobic submerged bioreactor has
been proved to be a potential device
for secondary treatment of
wastewater.
 The rate of remediation of
wastewater depends on the intensity
of bacterial growth.
 High salinity retards the remediation
process i.e. the bacterial growth.

26. Conclusion
 The experimental results indicate
that spherical shaped. i.e. the small
size tire chips are better for bio-
remediation for landfill leachate
treatment than rectangular shape.
 In submergence bioreactor clogging
rate is slower than trickling filter
and clogging can be eliminated
through backwashing .